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The Distribution of Sialic Acids Within the Eukaryotic Cell

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Biological Roles of Sialic Acid

Abstract

In this chapter the distribution of sialic acid within the cell will be discussed. It is fairly safe to assume that essentially all of the sialic acid of the cell is in a bound form incorporated in glycoproteins and glycolipids and that these are to a large extent components of the cell membranes. In glandular secretory tissues large amounts of macromolecules containing sialic acid may be present in granular from awaiting elimination from the cell—a rather special situation that will not be considered further.

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References

  • Atterfelt, P., Blohme, I., Norrby, A., and Svennerholm, L., 1958, Sialic acids of hog gastric mucosa, Acta Chem. Scand. 12: 359–360.

    Article  CAS  Google Scholar 

  • Barton, N. W., and Rosenberg, A., 1973, Action of Vibrio cholerae neuraminidase (sialidase) upon the surface of intact cells and their isolated sialolipid components, J. Biol. Chem. 248: 7353–7358.

    PubMed  CAS  Google Scholar 

  • Bennett, H. S., 1963, Morphological aspects of extracellular polysaccharides, J. Histochem. Cytochem. 11: 15.

    Article  Google Scholar 

  • Blix, G., 1936, Ãœber die Kohlenhydratgruppen des Submaxillarismucins, Hope-Seyl. Z. 240: 43–53.

    Article  CAS  Google Scholar 

  • Bosmann, H. B., 1972, Sialyl transferase activity in normal and RNA and DNA virus transformed cells utilizing desialyzed, trypsinized cell plasma membrane external surface glycoproteins as exogenous acceptors, Biochem. Biophys. Res. Commun. 49: 1256.

    Article  PubMed  CAS  Google Scholar 

  • Bosmann, H. B., and Martin, S. S., 1969, Mitochondrial autonomy, incorporation of monosaccharides into glycoprotein by isolated mitochondria, Science 164: 190.

    Article  PubMed  CAS  Google Scholar 

  • Bosmann, H. B., Hagopian, A., and Eylar, E. H., 1968, Cellular membranes: the isolation and characterization of the plasma and smooth membranes of HeLa Cells, Arch. Biochem. Biophys. 128: 51–69.

    Article  PubMed  CAS  Google Scholar 

  • Bourrillon, R., 1972, Urinary Glycoproteins, Glycopeptides and Related Heterosaccharides in: Glycoproteins (Gottschalk, A., ed.), pp. 909–925 Elsevier Publishing Co., Amsterdam.

    Google Scholar 

  • Brunngraber, E. G., and Brown, B. D., 1963, Preparation of sialomucopolysaccharides from brain mitochondrial fraction, Biochim. Biophys. Acta 69: 581–582.

    Article  PubMed  CAS  Google Scholar 

  • Brunngraber, E. G., and Brown, B. D., 1964a, Heterogeneity of sialomucopolysaccharides prepared from whole rat brain, Biochim. Biophys. Acta 83: 357–360.

    PubMed  CAS  Google Scholar 

  • Brunngraber, E. G., and Brown, B. D., 1964b, Fractionation of brain macromolecules. II. Isolation of protein-linked sialomucopolysaccharides from subcellular, particulate fractions from rat brain, J. Neurochem. 11: 449–459.

    Article  PubMed  CAS  Google Scholar 

  • Buck, C. A., Glick, M. C., and Warren, L., 1970, A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells, Biochemistry 9: 4567–4575.

    Article  PubMed  CAS  Google Scholar 

  • Buck, C. A., Fuhrer, J. P., Soslau, G., and Warren, L., 1974, Membrane glycopeptides from subcellular fractions of control and virus-transformed cells, J. Biol. Chem. 249: 1541–1550.

    PubMed  CAS  Google Scholar 

  • Burton, R. M., Howard, R. E., Baer, S., and Balfour, Y. M., 1965, Gangliosides and acetylcholine of the central nervous system, Biochim. Biophys. Acta 84: 441–447.

    Google Scholar 

  • Codington, J. F., Sanford, B. H., and Jeanloz, R. W., 1970, Glycoprotein coat of the TA3cell. I. Removal of carbohydrate and protein material from viable cells, J. Natl. Cancer Inst. 45: 637–647.

    PubMed  CAS  Google Scholar 

  • Cook, G. M. W., Seaman, G. V. F., and Weiss, L., 1963, Physicochemical differences between ascites and solid forms of sarcoma 37 cells, Cancer Res. 23: 1813–1818.

    PubMed  CAS  Google Scholar 

  • de Bernard, B., Prigliarello, M. C., Sandri, G., Sottocasa, G. L., and Vittur, F., 1971, Glycoprotein components, sialic acid and hexosamines, bound to the inner and outer mitochondrial membranes, FEBS Lett. 12: 125–128.

    Article  PubMed  CAS  Google Scholar 

  • de Duve, C., and Wattiaux, R., 1966, Functions of lysosomes, Ann. Rev. Physiol. 28: 435–492.

    Article  CAS  Google Scholar 

  • Dod, B. J., and Gray, G. M., 1968a, The lipid composition of rat-liver plasma membrane, Biochim. Biophys. Acta 150: 397–404.

    Article  PubMed  CAS  Google Scholar 

  • Dod, B. J., and Gray, G. M., 1968b, The localization of the neutral glycosphingolipids in rat liver cells, Biochem. J. 110: 50P.

    Google Scholar 

  • Eichberg, J., Whittaker, V. P., and Dawson, R. M. C., 1964, Distribution of lipids in subcellular particular particles of guinea pig brain, Biochem. J. 92: 91–99.

    PubMed  CAS  Google Scholar 

  • Eylar, E. H., and Mad off, M. A., 1962, Isolation of a glycopeptide from the red cell membrane, Fed. Proc. 21: 402.

    Google Scholar 

  • Eylar, E. H., Madoff, M. A., Brody, O. V., and Oncley, J. L., 1962, The contribution of sialic acid to the surface charge of the erythroycte, J. Biol. Chem. 237: 1992–2000.

    PubMed  CAS  Google Scholar 

  • Emmelot, P., and Bos, C. J., 1972, Studies on plasma membranes, VVII. On the chemical composition of plasma membranes prepared from rat and mouse liver and hepatomas, J. Membrane Biol. 9: 83–104.

    Article  CAS  Google Scholar 

  • Gasic, G., and Berwick, L., 1962, Hale stain for sialic acid containing mucins—Adaptation to electron microscopy, J. Cell Biol. 19: 223–228.

    Article  Google Scholar 

  • Gasic, G., and Gasic, T., 1963, Removal of PAS positive surface sugars in tumor cells, by glycosidases, Proc. Soc. Exp. Biol. Med. 114: 660–663.

    PubMed  CAS  Google Scholar 

  • Glick, M. C., Comstock, C., and Warren, L., 1970, Membranes of animal cells. VII. Carbohydrates of surface membranes and whole cells, Biochim. Biophys. Acta 219: 290–300.

    Article  PubMed  CAS  Google Scholar 

  • Glick, M. C., Comstock, C. A., Cohen, M. A., and Warren, L., 1971, Membranes of animal cells. 8. Distribution of sialic acid, hexosamines and sialidase in the L cell, Biochim. Biophys. Acta 233: 247–257.

    Article  PubMed  CAS  Google Scholar 

  • Gottschalk, A., 1960, The Chemistry and Biology of Sialic Acids and Related Substances, Cambridge University Press, London.

    Google Scholar 

  • Gottschalk, A., 1972, Glycoproteins, Elsevier Publishing Co., Amsterdam.

    Google Scholar 

  • Hotta, K., Hamazaki, H., Kurokawa, M., and Isaka, S., 1970a, Isolation and properties of a new type of sialopolysaccharide-protein complex from the jelly coat of sea urchin eggs, J. Biol. Chem. 245: 5434–5440.

    PubMed  CAS  Google Scholar 

  • Hotta, K., Kurokawa, M., and Isaka, S., 1970b, Isolation and identification of two sialic acids from the jelly coat of sea urchin eggs, J. Biol. Chem. 245: 6307–6311.

    PubMed  CAS  Google Scholar 

  • Hotta, K., Kurokawa, M., and Isaka, S., 1973, A novel sialic acid and fucose containing disaccharide isolated from the jelly coat of sea urchin eggs, J. Biol. Chem. 248: 629–631.

    PubMed  CAS  Google Scholar 

  • Immers, J., 1968, N-acetylneuraminic acid in the sea urchin jelly coat, Acta Chem. Scand. 22: 2046–2048.

    Article  PubMed  CAS  Google Scholar 

  • Kashnig, D. M., and Kasper, D. M., 1969, Isolation, Morphology, and composition of the nuclear membrane from rat liver, J. Biol. Chem. 244: 3786–3792.

    PubMed  CAS  Google Scholar 

  • Kean, E. L., 1970, Nuclear cytidine 5′-monophosphosialic acid synthetase, J. Biol. Chem. 245: 2301–2308.

    PubMed  CAS  Google Scholar 

  • Kraemer, P. M., 1965, Sialic acid of mammalian cell lines, J. Cell Physiol. 67: 23–34.

    Article  Google Scholar 

  • Lapetina, E. G., Soto, E. F., and DeRobertis, E., 1967, Gangliosides and acetylcholinesterase in isolated membranes of the rat-brain cortex, Biochim. Biophys. Acta 135: 33–43.

    Article  PubMed  CAS  Google Scholar 

  • Lichtman, M. A., and Weed, R. I., 1970, Electrophoretic mobility and N-acetylneuraminic acid content of human normal and leukemic lymphocytes and granulocytes, Blood 35: 12–22.

    PubMed  CAS  Google Scholar 

  • Makita, A., and Seyama, Y., 1971, Alterations of Forssman-antigenic reactivity and monosaccharide composition in plasma membrane from polyoma-transformed hamster cells, Biochim. Biophys. Acta 241: 403–411.

    Article  PubMed  CAS  Google Scholar 

  • Marcus, P. I., Salb, J. M., and Schwartz, V. G., 1965, Nuclear surface N-acetylneuraminic acid terminating receptors for myxovirus attachment, Nature 208: 1122–1124.

    Article  CAS  Google Scholar 

  • Meezan, E., Wu, H. C., Black, P. H., and Robbins, P. W., 1969, Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography, Biochem. 8: 2518–2524.

    Article  CAS  Google Scholar 

  • Miller, A., Sullivan, J. F., and Katz, J. H., 1963, Sialic acid content of the erythrocyte and of an ascites tumor cell of the mouse, Cancer Res. 23: 485.

    CAS  Google Scholar 

  • Molnar, J., 1967, Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of (14C)glucosamine and (14C)sialic acid into membrane proteins, Biochemistry 6: 3064–3075.

    Article  PubMed  CAS  Google Scholar 

  • Nordling, S. E., and Mayhew, E., 1966, On the intracellular uptake of neuraminidase, Exp. Cell Res. 44: 552–562.

    Article  PubMed  CAS  Google Scholar 

  • Noseworthy, J. R., Korchak, H., and Karnovsky, M. L., 1972, Phagocytosis and the sialic acid of the surface of polymorphonuclear leukocytes, J. Cell Physiol. 79: 91–96.

    Article  PubMed  CAS  Google Scholar 

  • Odin, L., 1955, Sialic acid in human cervical mucus, in hog seminal gel, and in ovomucin, Acta Chem. Scand. 9: 1235–1237.

    Article  CAS  Google Scholar 

  • Ohta, N., Pardee, A. B., McAuslan, B. R., and Burger, M. M., 1968, Sialic acid contents and controls of normal and malignant cells, Biochim. et Biophys. Acta 158: 98–102.

    Article  CAS  Google Scholar 

  • Patterson, M. K., and Touster, O., 1962, Intracellular distribution of sialic acid and its relationship to membrane, Biochim. Biophys. Acta 56: 626–628.

    Article  PubMed  CAS  Google Scholar 

  • Pricer, W. E., Jr., and Ashwell, G., 1971, The binding of desialylated glycoproteins by plasma membranes of rat liver, J. Biol. Chem. 69: 1838.

    Google Scholar 

  • Purdue, J. F., Kletzien, R., and Miller, K., 1971a, The isolation and characterization of plasma membrane from cultured cells. I. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted chick embryo fibroblasts, Biochim. Biophys. Acta 249: 419–434.

    Article  Google Scholar 

  • Purdue, J. F., Kletzien, R., Miller, K., Pridmore, G., and Wray, V. L., 1971b, The isolation and characterization of plasma membrane from cultured cells. II. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted parenchyma-like cells, Biochim. Biophys. Acta 249: 435–453.

    Article  Google Scholar 

  • Purdue, J. F., Warner, D., and Miller, K., 1973, The isolation and characterization of plasma membrane from cultured cells. III. The chemical composition of plasma membrane isolated from chicken tumors initiated with virus-transformed cells, Biochim. Biophys. Acta 298: 817–826.

    Article  Google Scholar 

  • Rambourg, A., and Leblond, C. P., 1967, Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat, J. Cell. Biol. 32: 27–53.

    Article  PubMed  CAS  Google Scholar 

  • Rambourg, A., 1969, Localisation ultrastructurale et nature du material colore au niveau de la surface cellulaire par le melange chromique-phosphotung-stique, J. Microscopie 8: 325–341.

    CAS  Google Scholar 

  • Renkonnen, O., Gahmberg, C. G., Simons, K., and Kääriäinen, L., 1970, Enrichment of gangliosides in plasma membranes of hamster kidney fibroblasts, Acta Chem. Scand. 24: 733–735.

    Article  Google Scholar 

  • Sakiyama, H., and Burge, B. W., 1972, Comparative studies of the carbohydratecontaining components of 3T3 and Simian virus 40 transformed 3T3 mouse fibroblasts, Biochemistry 11: 1366–1376.

    Article  PubMed  CAS  Google Scholar 

  • Sheinin, R., and Onodera, K., Yogeeswaran, G., and Murray, R. K., Studies of components of the surface of normal and virus-transformed mouse cells, in: Lepetit Colloquia on Biology and Medicine. 2. The Biology of Oncogenic Viruses, 1971, (L. G. Silvestri, ed.), pp. 274–285, North-Holland Publishing Co., Amsterdam.

    Google Scholar 

  • Shimizu, S., and Funakoshi, T. 1970, Carbohydrate composition of the plasma membranes of rat ascites hepatoma, Biochim. Biophys. Acta 203: 167–169.

    Article  PubMed  CAS  Google Scholar 

  • Stahl, W. L., and Trams, E. G., 1968, Synthesis of lipids by liver plasma membranes. Incorporation of acyl-coenzyme derivatives into membrane lipids in vitro, Biochim. Biophys. Acta 163: 459–471.

    Article  PubMed  CAS  Google Scholar 

  • Svennerholm, L., 1958, Quantitative estimation of sialic acid. III. An anion exchangeresin method, Acta Chem. Scand. 12: 547–554.

    Article  CAS  Google Scholar 

  • Thines-Sempoux, D., 1967, Chemical similarities between the lysosome and plasma membranes, Biochem. J. 105: 20p–21p.

    CAS  Google Scholar 

  • Uzman, L. L., and Rumley, M. K., 1956, Neuraminic acid as a constituent of human cerebrospinal fluid, Proc. Soc. Exp. Biol. Med. 93: 497–500.

    PubMed  CAS  Google Scholar 

  • Van Blitterswijk, J., Emmelot, P., and Feltcamp, C. A., 1973, Studies on plasma membranes. XIX. Isolation and characterization of a plasma membrane fraction from calf thymocytes, Biochim. Biophys. Acta 298: 577–592.

    Article  PubMed  Google Scholar 

  • Wallach, D. F. H., and Eylar, E. J., 1961, Sialic acid in the cellular membranes of Ehrlich ascites-carcinoma cells, Biochim. Biophys. Acta 52: 594–596.

    Article  PubMed  CAS  Google Scholar 

  • Wallach, D. F. H., and Kamat, V. B., 1966, The contribution of sialic acid to the surface change of fragments of plasma membrane and endoplasmic reticulum, J. Cell Biol. 30: 660–663.

    Article  PubMed  CAS  Google Scholar 

  • Warren, L., 1959, Sialic acid in human semen and in the male genital tract, J. Clin. Invest. 38: 755–761.

    Article  PubMed  CAS  Google Scholar 

  • Warren, L., 1960a, Unbound sialic acids in fish eggs, Biochim. Biophys. Acta 44: 347–351.

    Article  PubMed  CAS  Google Scholar 

  • Warren, L., 1960b, Studies on sialic acids, Fed. Proc. 19: 147.

    Google Scholar 

  • Warren, L., and Glick, M. C., 1969, Isolation of surface membranes of tissue culture cells in: Fundamental Techniques in Virology, Part II, Preparation of subcellular fractions, (Habel, K. and Salzman, N. P., eds.), p. 66–71, Academic Press, New York.

    Google Scholar 

  • Warren, L., Fuhrer, J. P. and Buck, C. A., 1972, Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth-dependent sialyl transferase, Proc. Natl. Acad. Sci. U.S. 69: 1838–1842.

    Article  CAS  Google Scholar 

  • Wherrett, J. R., and Mcllwain, H., 1962, Gangliosides, phospholipids, protein and ribonucleic acid in subfractions of cerebral microsomal material, Biochem. J. 84: 232–237.

    PubMed  CAS  Google Scholar 

  • Weigandt, H., 1967, The subcellular localization of gangliosides in the brain, J. Neurochem. 14: 671–674.

    Article  Google Scholar 

  • Weinstein, D., Marsh, J., Glick, M. C., and Warren, L., 1969, Membranes of animal cells. IV. Lipids of the L cell and its surface membrane, J. Biol. Chem. 244: 4103–4111.

    PubMed  CAS  Google Scholar 

  • Weinstein, D., Marsh, J., Glick, M. C., and Warren, L., 1970, Membranes of animal cells. VI. The glycolipids of the L cell and its surface membrane, J. Biol. Chem. 245: 3928–3937.

    PubMed  CAS  Google Scholar 

  • Weiss, L., 1967, The Cell Periphery, Metastasis and Other Contact Phenomena, pp. 267–270, John Wiley, New York.

    Google Scholar 

  • Werner, I., 1953, Studies on glycoproteins from mucous epithelium and epithelial secretions, Acta Soc. Med. Upsalien 58: 1–55.

    CAS  Google Scholar 

  • Wesemann, W., Henkel, R., and Marx, R., 1971, Receptors of neurotransmitters. V. Sialic acid distribution and characterization of the 5-hydroxytryptamine receptor in synaptic structures, Biochem. Pharm. 20: 1961–1966.

    Article  PubMed  CAS  Google Scholar 

  • Wintzer, G., and Uhlenbruck, G., 1967, Topochemische Anordnung von Gangliosiden in der Erythrozyten-membran, Immunitatsforsch. Allerg. 133: 60–67.

    CAS  Google Scholar 

  • Winzler, R. J., Harris, E. D., Pekas, D. J., Johnson, C. A., and Weber, P., 1967, Studies on glycopeptides released by trypsin from intact human erythrocytes, Biochemistry 6: 2195–2201.

    Article  PubMed  CAS  Google Scholar 

  • Wolfe, L. S., 1961, The distribution of gangliosides in subcellular fractions of guinea-pig cerebral cortex, Biochem. J. 79: 348–355.

    PubMed  CAS  Google Scholar 

  • Woodin, A. M., and Wieneki, A. A., 1966, Composition and properties of a cellmembrane fraction from the polymorphonuclear leucocyte, Biochem. J. 99: 493–500.

    PubMed  CAS  Google Scholar 

  • Wu, H. C., Meezan, E., Black, P. H., and Robbins, P. W., 1969, Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. I. Glucosamine-labeling patterns in 3T3, spontaneously transformed 3T3, and SV-40-transformed 3T3 cells, Biochemistry 8: 2509–2517.

    Article  PubMed  CAS  Google Scholar 

  • Yamashina, I., Izumi, K., Okawa, H., and Furuya, E., 1965, Hexosamine and sialic acid in mitochondria and microsomes from rabbit liver, J. Biochem. 58: 538–542.

    PubMed  CAS  Google Scholar 

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Warren, L. (1976). The Distribution of Sialic Acids Within the Eukaryotic Cell. In: Rosenberg, A., Schengrund, CL. (eds) Biological Roles of Sialic Acid. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2226-9_3

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  • DOI: https://doi.org/10.1007/978-1-4684-2226-9_3

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