Abstract
In this chapter the distribution of sialic acid within the cell will be discussed. It is fairly safe to assume that essentially all of the sialic acid of the cell is in a bound form incorporated in glycoproteins and glycolipids and that these are to a large extent components of the cell membranes. In glandular secretory tissues large amounts of macromolecules containing sialic acid may be present in granular from awaiting elimination from the cell—a rather special situation that will not be considered further.
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References
Atterfelt, P., Blohme, I., Norrby, A., and Svennerholm, L., 1958, Sialic acids of hog gastric mucosa, Acta Chem. Scand. 12: 359–360.
Barton, N. W., and Rosenberg, A., 1973, Action of Vibrio cholerae neuraminidase (sialidase) upon the surface of intact cells and their isolated sialolipid components, J. Biol. Chem. 248: 7353–7358.
Bennett, H. S., 1963, Morphological aspects of extracellular polysaccharides, J. Histochem. Cytochem. 11: 15.
Blix, G., 1936, Über die Kohlenhydratgruppen des Submaxillarismucins, Hope-Seyl. Z. 240: 43–53.
Bosmann, H. B., 1972, Sialyl transferase activity in normal and RNA and DNA virus transformed cells utilizing desialyzed, trypsinized cell plasma membrane external surface glycoproteins as exogenous acceptors, Biochem. Biophys. Res. Commun. 49: 1256.
Bosmann, H. B., and Martin, S. S., 1969, Mitochondrial autonomy, incorporation of monosaccharides into glycoprotein by isolated mitochondria, Science 164: 190.
Bosmann, H. B., Hagopian, A., and Eylar, E. H., 1968, Cellular membranes: the isolation and characterization of the plasma and smooth membranes of HeLa Cells, Arch. Biochem. Biophys. 128: 51–69.
Bourrillon, R., 1972, Urinary Glycoproteins, Glycopeptides and Related Heterosaccharides in: Glycoproteins (Gottschalk, A., ed.), pp. 909–925 Elsevier Publishing Co., Amsterdam.
Brunngraber, E. G., and Brown, B. D., 1963, Preparation of sialomucopolysaccharides from brain mitochondrial fraction, Biochim. Biophys. Acta 69: 581–582.
Brunngraber, E. G., and Brown, B. D., 1964a, Heterogeneity of sialomucopolysaccharides prepared from whole rat brain, Biochim. Biophys. Acta 83: 357–360.
Brunngraber, E. G., and Brown, B. D., 1964b, Fractionation of brain macromolecules. II. Isolation of protein-linked sialomucopolysaccharides from subcellular, particulate fractions from rat brain, J. Neurochem. 11: 449–459.
Buck, C. A., Glick, M. C., and Warren, L., 1970, A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells, Biochemistry 9: 4567–4575.
Buck, C. A., Fuhrer, J. P., Soslau, G., and Warren, L., 1974, Membrane glycopeptides from subcellular fractions of control and virus-transformed cells, J. Biol. Chem. 249: 1541–1550.
Burton, R. M., Howard, R. E., Baer, S., and Balfour, Y. M., 1965, Gangliosides and acetylcholine of the central nervous system, Biochim. Biophys. Acta 84: 441–447.
Codington, J. F., Sanford, B. H., and Jeanloz, R. W., 1970, Glycoprotein coat of the TA3cell. I. Removal of carbohydrate and protein material from viable cells, J. Natl. Cancer Inst. 45: 637–647.
Cook, G. M. W., Seaman, G. V. F., and Weiss, L., 1963, Physicochemical differences between ascites and solid forms of sarcoma 37 cells, Cancer Res. 23: 1813–1818.
de Bernard, B., Prigliarello, M. C., Sandri, G., Sottocasa, G. L., and Vittur, F., 1971, Glycoprotein components, sialic acid and hexosamines, bound to the inner and outer mitochondrial membranes, FEBS Lett. 12: 125–128.
de Duve, C., and Wattiaux, R., 1966, Functions of lysosomes, Ann. Rev. Physiol. 28: 435–492.
Dod, B. J., and Gray, G. M., 1968a, The lipid composition of rat-liver plasma membrane, Biochim. Biophys. Acta 150: 397–404.
Dod, B. J., and Gray, G. M., 1968b, The localization of the neutral glycosphingolipids in rat liver cells, Biochem. J. 110: 50P.
Eichberg, J., Whittaker, V. P., and Dawson, R. M. C., 1964, Distribution of lipids in subcellular particular particles of guinea pig brain, Biochem. J. 92: 91–99.
Eylar, E. H., and Mad off, M. A., 1962, Isolation of a glycopeptide from the red cell membrane, Fed. Proc. 21: 402.
Eylar, E. H., Madoff, M. A., Brody, O. V., and Oncley, J. L., 1962, The contribution of sialic acid to the surface charge of the erythroycte, J. Biol. Chem. 237: 1992–2000.
Emmelot, P., and Bos, C. J., 1972, Studies on plasma membranes, VVII. On the chemical composition of plasma membranes prepared from rat and mouse liver and hepatomas, J. Membrane Biol. 9: 83–104.
Gasic, G., and Berwick, L., 1962, Hale stain for sialic acid containing mucins—Adaptation to electron microscopy, J. Cell Biol. 19: 223–228.
Gasic, G., and Gasic, T., 1963, Removal of PAS positive surface sugars in tumor cells, by glycosidases, Proc. Soc. Exp. Biol. Med. 114: 660–663.
Glick, M. C., Comstock, C., and Warren, L., 1970, Membranes of animal cells. VII. Carbohydrates of surface membranes and whole cells, Biochim. Biophys. Acta 219: 290–300.
Glick, M. C., Comstock, C. A., Cohen, M. A., and Warren, L., 1971, Membranes of animal cells. 8. Distribution of sialic acid, hexosamines and sialidase in the L cell, Biochim. Biophys. Acta 233: 247–257.
Gottschalk, A., 1960, The Chemistry and Biology of Sialic Acids and Related Substances, Cambridge University Press, London.
Gottschalk, A., 1972, Glycoproteins, Elsevier Publishing Co., Amsterdam.
Hotta, K., Hamazaki, H., Kurokawa, M., and Isaka, S., 1970a, Isolation and properties of a new type of sialopolysaccharide-protein complex from the jelly coat of sea urchin eggs, J. Biol. Chem. 245: 5434–5440.
Hotta, K., Kurokawa, M., and Isaka, S., 1970b, Isolation and identification of two sialic acids from the jelly coat of sea urchin eggs, J. Biol. Chem. 245: 6307–6311.
Hotta, K., Kurokawa, M., and Isaka, S., 1973, A novel sialic acid and fucose containing disaccharide isolated from the jelly coat of sea urchin eggs, J. Biol. Chem. 248: 629–631.
Immers, J., 1968, N-acetylneuraminic acid in the sea urchin jelly coat, Acta Chem. Scand. 22: 2046–2048.
Kashnig, D. M., and Kasper, D. M., 1969, Isolation, Morphology, and composition of the nuclear membrane from rat liver, J. Biol. Chem. 244: 3786–3792.
Kean, E. L., 1970, Nuclear cytidine 5′-monophosphosialic acid synthetase, J. Biol. Chem. 245: 2301–2308.
Kraemer, P. M., 1965, Sialic acid of mammalian cell lines, J. Cell Physiol. 67: 23–34.
Lapetina, E. G., Soto, E. F., and DeRobertis, E., 1967, Gangliosides and acetylcholinesterase in isolated membranes of the rat-brain cortex, Biochim. Biophys. Acta 135: 33–43.
Lichtman, M. A., and Weed, R. I., 1970, Electrophoretic mobility and N-acetylneuraminic acid content of human normal and leukemic lymphocytes and granulocytes, Blood 35: 12–22.
Makita, A., and Seyama, Y., 1971, Alterations of Forssman-antigenic reactivity and monosaccharide composition in plasma membrane from polyoma-transformed hamster cells, Biochim. Biophys. Acta 241: 403–411.
Marcus, P. I., Salb, J. M., and Schwartz, V. G., 1965, Nuclear surface N-acetylneuraminic acid terminating receptors for myxovirus attachment, Nature 208: 1122–1124.
Meezan, E., Wu, H. C., Black, P. H., and Robbins, P. W., 1969, Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography, Biochem. 8: 2518–2524.
Miller, A., Sullivan, J. F., and Katz, J. H., 1963, Sialic acid content of the erythrocyte and of an ascites tumor cell of the mouse, Cancer Res. 23: 485.
Molnar, J., 1967, Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of (14C)glucosamine and (14C)sialic acid into membrane proteins, Biochemistry 6: 3064–3075.
Nordling, S. E., and Mayhew, E., 1966, On the intracellular uptake of neuraminidase, Exp. Cell Res. 44: 552–562.
Noseworthy, J. R., Korchak, H., and Karnovsky, M. L., 1972, Phagocytosis and the sialic acid of the surface of polymorphonuclear leukocytes, J. Cell Physiol. 79: 91–96.
Odin, L., 1955, Sialic acid in human cervical mucus, in hog seminal gel, and in ovomucin, Acta Chem. Scand. 9: 1235–1237.
Ohta, N., Pardee, A. B., McAuslan, B. R., and Burger, M. M., 1968, Sialic acid contents and controls of normal and malignant cells, Biochim. et Biophys. Acta 158: 98–102.
Patterson, M. K., and Touster, O., 1962, Intracellular distribution of sialic acid and its relationship to membrane, Biochim. Biophys. Acta 56: 626–628.
Pricer, W. E., Jr., and Ashwell, G., 1971, The binding of desialylated glycoproteins by plasma membranes of rat liver, J. Biol. Chem. 69: 1838.
Purdue, J. F., Kletzien, R., and Miller, K., 1971a, The isolation and characterization of plasma membrane from cultured cells. I. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted chick embryo fibroblasts, Biochim. Biophys. Acta 249: 419–434.
Purdue, J. F., Kletzien, R., Miller, K., Pridmore, G., and Wray, V. L., 1971b, The isolation and characterization of plasma membrane from cultured cells. II. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted parenchyma-like cells, Biochim. Biophys. Acta 249: 435–453.
Purdue, J. F., Warner, D., and Miller, K., 1973, The isolation and characterization of plasma membrane from cultured cells. III. The chemical composition of plasma membrane isolated from chicken tumors initiated with virus-transformed cells, Biochim. Biophys. Acta 298: 817–826.
Rambourg, A., and Leblond, C. P., 1967, Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat, J. Cell. Biol. 32: 27–53.
Rambourg, A., 1969, Localisation ultrastructurale et nature du material colore au niveau de la surface cellulaire par le melange chromique-phosphotung-stique, J. Microscopie 8: 325–341.
Renkonnen, O., Gahmberg, C. G., Simons, K., and Kääriäinen, L., 1970, Enrichment of gangliosides in plasma membranes of hamster kidney fibroblasts, Acta Chem. Scand. 24: 733–735.
Sakiyama, H., and Burge, B. W., 1972, Comparative studies of the carbohydratecontaining components of 3T3 and Simian virus 40 transformed 3T3 mouse fibroblasts, Biochemistry 11: 1366–1376.
Sheinin, R., and Onodera, K., Yogeeswaran, G., and Murray, R. K., Studies of components of the surface of normal and virus-transformed mouse cells, in: Lepetit Colloquia on Biology and Medicine. 2. The Biology of Oncogenic Viruses, 1971, (L. G. Silvestri, ed.), pp. 274–285, North-Holland Publishing Co., Amsterdam.
Shimizu, S., and Funakoshi, T. 1970, Carbohydrate composition of the plasma membranes of rat ascites hepatoma, Biochim. Biophys. Acta 203: 167–169.
Stahl, W. L., and Trams, E. G., 1968, Synthesis of lipids by liver plasma membranes. Incorporation of acyl-coenzyme derivatives into membrane lipids in vitro, Biochim. Biophys. Acta 163: 459–471.
Svennerholm, L., 1958, Quantitative estimation of sialic acid. III. An anion exchangeresin method, Acta Chem. Scand. 12: 547–554.
Thines-Sempoux, D., 1967, Chemical similarities between the lysosome and plasma membranes, Biochem. J. 105: 20p–21p.
Uzman, L. L., and Rumley, M. K., 1956, Neuraminic acid as a constituent of human cerebrospinal fluid, Proc. Soc. Exp. Biol. Med. 93: 497–500.
Van Blitterswijk, J., Emmelot, P., and Feltcamp, C. A., 1973, Studies on plasma membranes. XIX. Isolation and characterization of a plasma membrane fraction from calf thymocytes, Biochim. Biophys. Acta 298: 577–592.
Wallach, D. F. H., and Eylar, E. J., 1961, Sialic acid in the cellular membranes of Ehrlich ascites-carcinoma cells, Biochim. Biophys. Acta 52: 594–596.
Wallach, D. F. H., and Kamat, V. B., 1966, The contribution of sialic acid to the surface change of fragments of plasma membrane and endoplasmic reticulum, J. Cell Biol. 30: 660–663.
Warren, L., 1959, Sialic acid in human semen and in the male genital tract, J. Clin. Invest. 38: 755–761.
Warren, L., 1960a, Unbound sialic acids in fish eggs, Biochim. Biophys. Acta 44: 347–351.
Warren, L., 1960b, Studies on sialic acids, Fed. Proc. 19: 147.
Warren, L., and Glick, M. C., 1969, Isolation of surface membranes of tissue culture cells in: Fundamental Techniques in Virology, Part II, Preparation of subcellular fractions, (Habel, K. and Salzman, N. P., eds.), p. 66–71, Academic Press, New York.
Warren, L., Fuhrer, J. P. and Buck, C. A., 1972, Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth-dependent sialyl transferase, Proc. Natl. Acad. Sci. U.S. 69: 1838–1842.
Wherrett, J. R., and Mcllwain, H., 1962, Gangliosides, phospholipids, protein and ribonucleic acid in subfractions of cerebral microsomal material, Biochem. J. 84: 232–237.
Weigandt, H., 1967, The subcellular localization of gangliosides in the brain, J. Neurochem. 14: 671–674.
Weinstein, D., Marsh, J., Glick, M. C., and Warren, L., 1969, Membranes of animal cells. IV. Lipids of the L cell and its surface membrane, J. Biol. Chem. 244: 4103–4111.
Weinstein, D., Marsh, J., Glick, M. C., and Warren, L., 1970, Membranes of animal cells. VI. The glycolipids of the L cell and its surface membrane, J. Biol. Chem. 245: 3928–3937.
Weiss, L., 1967, The Cell Periphery, Metastasis and Other Contact Phenomena, pp. 267–270, John Wiley, New York.
Werner, I., 1953, Studies on glycoproteins from mucous epithelium and epithelial secretions, Acta Soc. Med. Upsalien 58: 1–55.
Wesemann, W., Henkel, R., and Marx, R., 1971, Receptors of neurotransmitters. V. Sialic acid distribution and characterization of the 5-hydroxytryptamine receptor in synaptic structures, Biochem. Pharm. 20: 1961–1966.
Wintzer, G., and Uhlenbruck, G., 1967, Topochemische Anordnung von Gangliosiden in der Erythrozyten-membran, Immunitatsforsch. Allerg. 133: 60–67.
Winzler, R. J., Harris, E. D., Pekas, D. J., Johnson, C. A., and Weber, P., 1967, Studies on glycopeptides released by trypsin from intact human erythrocytes, Biochemistry 6: 2195–2201.
Wolfe, L. S., 1961, The distribution of gangliosides in subcellular fractions of guinea-pig cerebral cortex, Biochem. J. 79: 348–355.
Woodin, A. M., and Wieneki, A. A., 1966, Composition and properties of a cellmembrane fraction from the polymorphonuclear leucocyte, Biochem. J. 99: 493–500.
Wu, H. C., Meezan, E., Black, P. H., and Robbins, P. W., 1969, Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. I. Glucosamine-labeling patterns in 3T3, spontaneously transformed 3T3, and SV-40-transformed 3T3 cells, Biochemistry 8: 2509–2517.
Yamashina, I., Izumi, K., Okawa, H., and Furuya, E., 1965, Hexosamine and sialic acid in mitochondria and microsomes from rabbit liver, J. Biochem. 58: 538–542.
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Warren, L. (1976). The Distribution of Sialic Acids Within the Eukaryotic Cell. In: Rosenberg, A., Schengrund, CL. (eds) Biological Roles of Sialic Acid. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2226-9_3
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DOI: https://doi.org/10.1007/978-1-4684-2226-9_3
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