Abstract
Since the advent of enzymic dissociation methods for the serial propagation of cells in culture numerous studies have detailed the changes in growth properties and morphology which take place with continued passage. Swim and associates [1, 2] clearly defined the characteristics of two distinct classes of cells which proliferate in vitro. One class, later termed primary cell lines by Harris [3] and others, comprises the apparently unaltered progeny of cells derived by dissociation of a given animal tissue. The second class, the so-called permanent cell lines, evolves during serial propagation of primary lines. We will be concerned mainly with the growth properties of primary cell lines in this discussion.
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References
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© 1970 Plenum Press, New York
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Hay, R.J. (1970). Cell Strain Senescence in Vitro: Cell Culture Anomaly or an Expression of a Fundamental Inability of Normal Cells to Survive and Proliferate. In: Holečková, E., Cristofalo, V.J. (eds) Aging in Cell and Tissue Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1821-7_2
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DOI: https://doi.org/10.1007/978-1-4684-1821-7_2
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