UV—visible spectrophotometry



The visible region of the spectrum ranges from 400–700 nm, which corresponds to frequencies of 7 × 1014–4 × 1014 Hz. Radiation at the low-energy high-wavelength end of the spectrum is perceived as red, while that at the high-energy part of the spectrum is blue. The spectral region between 180–400 nm corresponds to the ultraviolet (UV) region.


Molecular Orbital Molar Absorption Coefficient Atomic Orbital Instrumental Analysis Derivative Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Cahill, J. and Retzik, M. (1985) Int. Lab. May, 48.Google Scholar
  2. Donovan, J.W. (1969) J. Biol. Chem. 244, 1961.Google Scholar
  3. Goodwin, T.W. and Mercer, E.I. (1983) Introduction to Plant Biochemistry, 2nd edn, Pergamon, Oxford.Google Scholar
  4. Headridge, J.B. (1961) Photometric Titrations,Pergamon, New York.Google Scholar
  5. IUPAC (1979) Standard Methods for the Analysis of Oils, Fats and Derivatives,6th edn, Pergamon, Oxford.Google Scholar
  6. Koch, A.L. (1981) Growth measurement, ch. 11, in Manual of Methods for General Bacteriology, Gerhardt, P. et al. (eds), American Society for Microbiology, Washington.Google Scholar

Further reading

  1. Grum, F. (1972) ch. 3, in Physical Methods of Chemistry, vol 1, pt III B, Weissberger, A. and Rossiter, B.W. (eds), Wiley-Interscience, New York.Google Scholar
  2. Morton, R.A. (1975) Biochemical Spectroscopy,vols. 1 and 2, Adam Hilger, London.Google Scholar
  3. Olsen, E.D. (1975) Modern Optical Methods of Analysis, McGraw-Hill, New York.Google Scholar
  4. Rao, C.N.R. (1975) Ultraviolet and Visible Spectroscopy (Chemical Applications), Butterworth, London.Google Scholar

Copyright information

© Blackie & Son Ltd 1987

Authors and Affiliations

  1. 1.University of ReadingUK

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