Skip to main content

Liquid chromatography

  • Chapter
  • 243 Accesses

Abstract

Chromatography, in its simplest form, may be described as a process that allows resolution of a mixture of compounds as a consequence of the different rates at which they move through a stationary phase, under the influence of a mobile phase. This general definition does not restrict the nature of the phases involved and indeed it is the wide range of such phases that makes the techniques of chromatography so diverse. For example, the mobile phase could be a gas passing over a stationary liquid phase, leading to gas-liquid chromatography, or both the mobile and stationary phases could be liquids leading to liquid-liquid partition chromatography. This chapter is concerned only with those techniques in which liquid mobile phases are employed; those techniques employing gas as the mobile phase are discussed in Chapter 3.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Calton G.J. (1984) Methods in Enzymology 104, 381.

    Article  Google Scholar 

  • Day, D.T. (1897) Am. Phil. Soc. 36, 112.

    Google Scholar 

  • Day, D.T. (1903) Science 17, 1007.

    Google Scholar 

  • De Leenheer, A.P., Lambert, W.E. and de Ruyter, M.G.M. (1985) Modern Chromatographic Analysis of Vitamins, Marcel Dekker, New York.

    Google Scholar 

  • Henschen, A., Hupe, K-P., Lottspeich, F. and Voelter, W. (1985) High Performance Liquid Chromatography in Biochemistry, VCH Verlagsgesellschaft, Weinheim.

    Google Scholar 

  • Izmailov, N.A. and Shraiber, M.S. (1938) Farmatsiya, 3, 1.

    Google Scholar 

  • Lawrence, J.F. (1984) Food Constituents and Food Residues, Marcel Dekker, New York.

    Google Scholar 

  • Macrae, R. (1982) HPLC in Food Analysis, Academic Press, London.

    Google Scholar 

  • McMurray, C.H. and Blanchflower, W.J. (1979) J. Chromatogr. 178, 525.

    Article  Google Scholar 

  • Martin, A.J.P. and Synge, R.L.M. (1941) Biochem. J. 35, 1358.

    Google Scholar 

  • Miller, J.N. (1978) In Chromatography of Synthetic Biological Polymers, R. Epton (ed.), Ellis Horwood, Chichester, 181.

    Google Scholar 

  • O’Hare, M.J. and Nice, E.C. (1979) J. Chromatogr. 171, 209.

    Article  Google Scholar 

  • Poole, C.F. and Schuette, S.A. (1984) Contemporary Practice of Chromatography, Elsevier, Amsterdam, ch. 1.

    Google Scholar 

  • Thompson, S.T., Cass, K.H. and Steilwagen, E. (1975) Proc. Nat. Acad. Sci. USA 72, 669.

    Article  Google Scholar 

  • Tswett, M. (1906) Ber. Dsch. Bot. Ges. 24, 384.

    Google Scholar 

Further reading

  • Grob, R.L. (1983) Chromatographic Analysis of the Environment, 2nd edn, Marcel Dekker, New York.

    Google Scholar 

  • Lurie, I.S. and Wittwer, J.D. (1983) High Performance Liquid Chromatography in Forensic Chemistry, Marcel Dekker, New York.

    Google Scholar 

  • Munson, J.W. (1984) Pharmaceutical Analysis, Marcel Dekker, New York.

    Google Scholar 

  • Provder, T. (1980) Size Exclusion Chromatography, American Chemical Society, Washington, DC.

    Book  Google Scholar 

  • Snyder, L.R. and Kirkland, J.J. (1979) Introduction to Modern Liquid Chromatography, 2nd edn. Wiley, New York.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Blackie & Son Ltd

About this chapter

Cite this chapter

Gordon, M.H., Macrae, R. (1987). Liquid chromatography. In: Instrumental Analysis in the Biological Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1521-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-1521-6_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-216-92010-1

  • Online ISBN: 978-1-4684-1521-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics