Liquid chromatography



Chromatography, in its simplest form, may be described as a process that allows resolution of a mixture of compounds as a consequence of the different rates at which they move through a stationary phase, under the influence of a mobile phase. This general definition does not restrict the nature of the phases involved and indeed it is the wide range of such phases that makes the techniques of chromatography so diverse. For example, the mobile phase could be a gas passing over a stationary liquid phase, leading to gas-liquid chromatography, or both the mobile and stationary phases could be liquids leading to liquid-liquid partition chromatography. This chapter is concerned only with those techniques in which liquid mobile phases are employed; those techniques employing gas as the mobile phase are discussed in Chapter 3.


Mobile Phase Stationary Phase Biological Science Instrumental Analysis Solute Molecule 
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  1. Calton G.J. (1984) Methods in Enzymology 104, 381.CrossRefGoogle Scholar
  2. Day, D.T. (1897) Am. Phil. Soc. 36, 112.Google Scholar
  3. Day, D.T. (1903) Science 17, 1007.Google Scholar
  4. De Leenheer, A.P., Lambert, W.E. and de Ruyter, M.G.M. (1985) Modern Chromatographic Analysis of Vitamins, Marcel Dekker, New York.Google Scholar
  5. Henschen, A., Hupe, K-P., Lottspeich, F. and Voelter, W. (1985) High Performance Liquid Chromatography in Biochemistry, VCH Verlagsgesellschaft, Weinheim.Google Scholar
  6. Izmailov, N.A. and Shraiber, M.S. (1938) Farmatsiya, 3, 1.Google Scholar
  7. Lawrence, J.F. (1984) Food Constituents and Food Residues, Marcel Dekker, New York.Google Scholar
  8. Macrae, R. (1982) HPLC in Food Analysis, Academic Press, London.Google Scholar
  9. McMurray, C.H. and Blanchflower, W.J. (1979) J. Chromatogr. 178, 525.CrossRefGoogle Scholar
  10. Martin, A.J.P. and Synge, R.L.M. (1941) Biochem. J. 35, 1358.Google Scholar
  11. Miller, J.N. (1978) In Chromatography of Synthetic Biological Polymers, R. Epton (ed.), Ellis Horwood, Chichester, 181.Google Scholar
  12. O’Hare, M.J. and Nice, E.C. (1979) J. Chromatogr. 171, 209.CrossRefGoogle Scholar
  13. Poole, C.F. and Schuette, S.A. (1984) Contemporary Practice of Chromatography, Elsevier, Amsterdam, ch. 1.Google Scholar
  14. Thompson, S.T., Cass, K.H. and Steilwagen, E. (1975) Proc. Nat. Acad. Sci. USA 72, 669.CrossRefGoogle Scholar
  15. Tswett, M. (1906) Ber. Dsch. Bot. Ges. 24, 384.Google Scholar

Further reading

  1. Grob, R.L. (1983) Chromatographic Analysis of the Environment, 2nd edn, Marcel Dekker, New York.Google Scholar
  2. Lurie, I.S. and Wittwer, J.D. (1983) High Performance Liquid Chromatography in Forensic Chemistry, Marcel Dekker, New York.Google Scholar
  3. Munson, J.W. (1984) Pharmaceutical Analysis, Marcel Dekker, New York.Google Scholar
  4. Provder, T. (1980) Size Exclusion Chromatography, American Chemical Society, Washington, DC.CrossRefGoogle Scholar
  5. Snyder, L.R. and Kirkland, J.J. (1979) Introduction to Modern Liquid Chromatography, 2nd edn. Wiley, New York.Google Scholar

Copyright information

© Blackie & Son Ltd 1987

Authors and Affiliations

  1. 1.University of ReadingUK

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