Advertisement

Mass spectrometry

Chapter
  • 176 Downloads

Abstract

Mass spectrometry is a powerful technique for the identification of pure compounds. It can also be used for confirmation of the purity of a sample and for quantitative analysis of mixtures. Ions are produced from sample molecules by various methods, including bombardment with a beam of electrons, and they are then accelerated and separated from ions of different molecular mass prior to detection. Often, the molecular or parent ion, which is produced from the sample molecule by loss of an electron, is observed together with a large number of ions (daughter ions) produced by characteristic fragmentations of the molecular ion. The molecular ion gives the relative molecular mass of the compound directly, while the fragmentation pattern provides a fingerprint which is used for identification of the molecule. The mass spectrum consists of a series of peaks of varying intensity plotted against the mass-to-charge ratio (m/z). Mass spectrometry combines high specificity with great sensitivity, since amounts of less than a picogram of some compounds can be detected.

Keywords

Mass Analyser Instrumental Analysis Relative Molecular Mass Semipermeable Membrane Field Ionization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blakley, C.R. and Vestal, M.L. (1983) Analvt. Chem. 55, 750.CrossRefGoogle Scholar
  2. Dempster, A.J. (1918) Phys. Rev. 11, 316.CrossRefGoogle Scholar
  3. Fales, H.M., Lloyd, A.M. and Milne, G.A. (1970) J. Amer. Chem. Soc. 92, 1590CrossRefGoogle Scholar
  4. Ferretti, A. and Flanagan, V.P. (1979) Lipids 14 (5), 483.CrossRefGoogle Scholar
  5. Gibbons, G.F., Goad, L.J. and Goodwin, T.W. (1968) Phytochem. 7, 983.CrossRefGoogle Scholar
  6. Johnson, E.G. and Nier, A.O. (1953) Phys. Rev. 91, 10.CrossRefGoogle Scholar
  7. Lichman, D. (1964) Res. Dev. 15 (2) 52.Google Scholar
  8. Mattauch, J. and Herzog, R.F.K. (1934) Z. Physik 89, 786.CrossRefGoogle Scholar
  9. Millington, D.S. (1980) New Mass Spectral Techniques for Organic and Biochemical Analysis, VGMicromass Ltd, Altrincham.Google Scholar
  10. Morgan, R.P., Beynon, J.H., Bateman, R.H. and Green, B.N. (1978) Int. J. Mass Spec. Ion Phys. 28, 171.CrossRefGoogle Scholar
  11. Winkler, H.V. and Beckey, H.D. (1972) Org. Mass Spectrum. 6, 655.CrossRefGoogle Scholar

Further reading

  1. Howe, I., Williams, D.H. and Bowen, R.D. (1981) Mass Spectrometry: Principles and Applications, 2nd edn, McGraw-Hill, New York.Google Scholar
  2. Karasek, F.W., Hutzinger, O. and Safe, S. (1985) Mass Spectrometry in Environmental Sciences, Plenum, New York.CrossRefGoogle Scholar
  3. McLafferty, F.W. (1980) Interpretation of Mass Spectra, 3rd edn, University Science Books, Mill Valley, CA.Google Scholar
  4. Merritt, C. Jr. and McEwen, C.N. (1979–80) Mass spectrometry, Parts A and B, in Practical Spectroscopy Series, vol. 3, Marcel Dekker, New York.Google Scholar
  5. Rose M.E. and Johnstone, R.A.W. (1982) Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, Cambridge.Google Scholar

Copyright information

© Blackie & Son Ltd 1987

Authors and Affiliations

  1. 1.University of ReadingUK

Personalised recommendations