Abstract
In order to examine the possible relationship between asbestiform fiber caused cytotoxicity/carcinogenicity of human mesothelial cells and the plastic nature of their cytoskeleton, we have observed the positioning of intermediate filaments and microfilaments in cultured normal human mesothelial cells before and after ingestion of amosite. In addition, we have examined the relative fidelity of the mitotic spindle of these cells. We have found that asbestiform fibers had no significant affect upon the distribution of cytoskeletal filaments. However, the filaments and/or their subunits appear to adsorb to asbestos fibers. Further, the physical presence of the fibers within the cell appears to cause mitotic spindle dysfunction. Therefore, in human mesothelial cells, the mechanism of asbestos damage may include interaction of cytoskeleton filaments with the fibers which, in turn, potentiates disruption of normal spindle function.
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© 1991 Plenum Press, New York
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Somers, A.N.A., Mason, E.A., Gerwin, B.I., Harris, C.C., Lechner, J.F. (1991). Effects of Amosite Asbestos Fibers on the Filaments Present in the Cytoskeleton of Primary Human Mesothelial Cells. In: Brown, R.C., Hoskins, J.A., Johnson, N.F. (eds) Mechanisms in Fibre Carcinogenesis. NATO ASI Series, vol 223. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1363-2_41
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DOI: https://doi.org/10.1007/978-1-4684-1363-2_41
Publisher Name: Springer, Boston, MA
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