Abstract
This paper reviews the most recent progress made in the in vitro studies since the micropropagation scheme developed by Debergh and Maene (1981). According to their scheme, transfer on a fresh medium is no longer needed for elongation and root induction purposes. It is sufficient to add an appropriate liquid medium on top of the old exhausted agar medium (Maene, 1985; Maene and Debergh, 1985a). Special attention has been given to the quality of shoots produced in vitro. These are very often physiologically and anatomically abnormal compared to in vivo produced plant material and there is very little difference between vitrified and normal looking plants. The reason(s) for these physiological and anatomical abnormalities of tissue-cultured plants have been examined. Different approaches to overcome acclimatisation problems have also been discussed. The most promising results are obtained by lowering the relative humidity in the in vitro culture-container caused, by bottom-cooling. As the transpiration in tissue-cultured plants is comparable to in vivo conditions, this new culture technique can be described as hydroculture under aseptic conditions. The system has been illustrated for a few ornamental plants.
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© 1989 Plenum Press, New York
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Maene, L.J. (1989). Tissue Culture of Ornamental Plants. In: Dhawan, V. (eds) Applications of Biotechnology in Forestry and Horticulture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1321-2_17
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DOI: https://doi.org/10.1007/978-1-4684-1321-2_17
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