Advertisement

Analysis of Proteins Secreted by the Human Endometrium InVivo and InVitro

  • David T. MacLaughlin
  • George S. Richardson
  • Nanette F. Santoro
  • Anne A. Hargraves
  • Hans H. Bauer
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 230)

Summary

Human uterine luminal fluids contain over two dozen proteins distinct from those of serum as detected by two dimensional gel electrophoresis and silver-type protein staining. Eighty-one percent of these uterine fluid proteins can be detected in vitro by radiolabeled methionine incorporation studies and the vast majority of these products are epithelial in origin. The major recognizable menstrual cycle phase-dependent change in the protein pattern in these gels was the appearance of a protein group (number 27) of approximately 25,000 mw and pi of 5.8 – 6.3. This group of proteins was found in nearly all mid- and all late secretory phase fluids or culture media and in none obtained earlier in the cycle. As yet, however, it has not been possible to induce these proteins in proliferative specimens in vitro by the addition of estrogens and/or progestins, though studies along these lines are continuing. Although we cannot be certain, it appears as though protein group number 27 is distinct from, but similar in several respects to, other proteins of human endometrium reported in the literature.

Keywords

Menstrual Cycle Protein Spot Human Endometrium Secretory Component Endometrial Gland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    P. E. Sylvan, D. T. MacLaughlin, G. S. Richardson, R. E. Scully, N. Nikrui, Human uterine luminal fluid proteins associated with secretory phase endometrium: progesterone-induced products?, Biol. Reprod. 24: 423–429 (1981).PubMedCrossRefGoogle Scholar
  2. 2.
    D. T. MacLaughlin, G. S. Richardson, Analysis of human uterine fluid proteins following radiolabeling by reductive methylation: comparison of proliferative and secretory phase samples, Biol. Reprod. 29: 733–742 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    D. A. Sullivan, G. S. Richardson, D. T. MacLaughlin, C. R. Wira, Variations in the levels of secretory component in human uterine fluid during the menstrual cycle, J. Steroid Biochem. 20: 509–514 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    D. T. MacLaughlin, N. F. Santoro, H. H. Bauer, D. Lawrence, G. S. Richardson, Two-dimensional gel electrophoresis of endometrial protein in human uterine fluids: qualitative and quantitative analysis, Biol. Reprod. 34: 579–585 (1986).PubMedCrossRefGoogle Scholar
  5. 5.
    S. G. Joshi, R. Rao, E. E. Henriques, R. S. Raiker, M. Gordon, Luteal phase concentrations of a progestagen-associated endometrial protein (PEP) in the serum of cycling women with adequate or inadequate endometrium, J. Clin. Endo. Metab. 65: 1247–1249 (1986).CrossRefGoogle Scholar
  6. 6.
    O. Janne, L. Kontula, T. Luukkainen, R. Vihko, Oestrogen induced progesterone receptors in human uterus, J. Steroid Biochem. 6: 501 (1975).PubMedCrossRefGoogle Scholar
  7. 7.
    M. I. Whitehead, P. T. Townsend, J. Pryse-Davies, T. A, Ryder, R. J. B,King, Effects of estrogens and progestins on the biochemistry and morphology of the postmenopausal endometrium, N. Engl. J. Med. 305: 1599–1605 (1981).PubMedCrossRefGoogle Scholar
  8. 8.
    D. R. Ciocca, R. H. Asch, D. J. Adams, W. L. McGuire, Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle, J. Clin. Endocrinol. Metab. 57: 496–499 (1983).PubMedCrossRefGoogle Scholar
  9. 9.
    I. A. Maslar, D. H. Riddick, Prolactin production by human endometrium during the menstrual cycle, Am. J. Qbstet. Gynecol. 135: 751–754 (1979).Google Scholar
  10. 10.
    D. C. Daly, I. A. Maslar, D. H. Riddick, Prolactin production during in vitro decidualization of proliferative endometrium, Am. J. Qbstet. Gynecol. 145: 672–678 (1983).Google Scholar
  11. 11.
    L. J. Heffner, D. A. Iddenden, C. R. Lyttle, Electrophoretic analyses of secreted human endometrial proteins: identification and characterization of luteal phase prolactin, J. Clin. Endocrinol. Metab. 62: 1288–1295 (1986).PubMedCrossRefGoogle Scholar
  12. 12.
    S. G. Joshi, A progestagen-associated protein of the human endometrium: basic studies and potential clinical applications, J. Steroid Biochem. 19: 751–757 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    L. Tseng, E. Gurpide, Stimulation of various 17b- and 20a-hydroxysteroid dehydrogenase activities by progestins in human endometrium, Endocrinology 104: 1745–1748 (1979).PubMedCrossRefGoogle Scholar
  14. 14.
    S. C. Bell, S. R. Patel, P. U. Kirwan, J. O. Drife, Protein synthesis and secretion by the human endometrium during the menstrual cycle and the effect of progesterone in vitro, J. Reprod. Fertil. 77: 221–231 (1986).PubMedCrossRefGoogle Scholar
  15. 15.
    S. T. Strinden, S. S. Shapiro, Progesterone-altered secretory proteins from cultured human endometrium, Endocrinology 112: 862–870 (1983).PubMedCrossRefGoogle Scholar
  16. 16.
    B. Casslen, A. Anderson, I. M. Nilsson, B. Astedt, Hormonal regulation of the release of plasminogen activator and of a specific activator inhibitor from endometrial tissue in culture, Proc. Soc. Exp. Biol. Med, (in press).Google Scholar
  17. 17.
    A. T. Noyes, A. T. Hertig, J. Rock, Dating the endometrial biopsy, Fertil. Steril. 1: 3–25 (1950).Google Scholar
  18. 18.
    L. C. Gravlee, Jet-irrigation method for the diagnosis of endometrial carcinoma, Obstet. Gynecol. 32: 168–173 (1969).Google Scholar
  19. 19.
    B. Casslen and B. Nilsson, Human uterine fluid, examined in indiluted samples for osmolarity and the concentration of inorganic ions, albumin, glucose, and urea, Am. J. Obstet. Gynecol. 150: 877–81 (1984).PubMedGoogle Scholar
  20. 20.
    O. H. Lowry, N. J. Roseborough, A. L. Farr, R. J. Randall, Protein measurement with folic phenol reagent, J. Biol. Chem. 193: 265–81 (1951).PubMedGoogle Scholar
  21. 21.
    I. Davidsohn and J. B. Henry, Clinical dianosis by laboratory methods, Saunder Publ, Philadelphia, 106–10 (1970).Google Scholar
  22. 22.
    P. H. O’Farrell, High resolution two-dimensional gel electrophoresis of proteins, J. Biol. Chem. 250: 4007–21 (1975).PubMedGoogle Scholar
  23. 23.
    C. N. Mariash, S. Seelig, J. Oppenheimer, A rapid inexpensive quantitative technique for the analysis of two-dimensional electrophoretograms, Anal. Biochem. 121: 388–94 (1982).PubMedCrossRefGoogle Scholar
  24. 24.
    D. Rodbard and D. M. Hutt, Statistical analysis of radioimmunoassay and immunoradiometric (labeled antibody) assays, in: Radioimmunoassay and Related Procedures in Medicine, Vol. I, International Atomic Energy Agency, Vienna, 165–92 (1974).Google Scholar
  25. 25.
    P. G. Satyaswaroop, K. S. Bressler, M. M. de la Pena, E. Gurpide, Isolation and culture of human endometrial glands, J. Clin. Endocrinol. Metab. 48: 639–641 (1979).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • David T. MacLaughlin
    • 1
  • George S. Richardson
    • 1
  • Nanette F. Santoro
    • 1
  • Anne A. Hargraves
    • 1
  • Hans H. Bauer
    • 1
  1. 1.Vincent Research Laboratory, Gynecology Service Massachusetts General Hospital, Departments of Obstetrics and Gynecology and SurgeryHarvard Medical SchoolBostonUSA

Personalised recommendations