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Hormonal Regulation of Rat Uterine Secretory Protein Synthesis

  • C. Richard Lyttle
  • Carol Wheeler
  • Barry S. Komm
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 230)

Summary

Treatment of the rat uterus with estradiol results in many morphological and biochemical changes. In order to examine the biochemical mechanisms of these changes, we are interested in finding a protein which can be used as an end-point indicator or marker. Estradiol administration results in the increased synthesis and release of a 115,000 and 65,000 dalton protein into the incubation media. In this paper, we demonstrate that these proteins are actually subunits of a larger protein having a molecular weight of 180,000 daltons. This protein appears to be specific to the uterus and may be produced mainly or solely by the epithelial cells. In the normal estrous cycle, maximal production is seen at estrus with essentially no production at diestrus. In the immature rat, synthesis of the 180K dalton protein is blocked by the interaction of progesterone and estradiol. However, unlike some uterine responses, the 180K protein is increased by treatment with several antiestrogens. This protein is also present in sufficient quantities to allow for the production of monoclonal antibodies. Thus this protein has many attributes of an excellent “marker protein” and will be of value in future studies aimed at elucidating the molecular mechanism(s) of steroid hormone action in the uterus.

Keywords

Estrous Cycle Estrogen Action Luminal Epithelium Luminal Epithelial Cell 180K Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • C. Richard Lyttle
    • 1
    • 2
  • Carol Wheeler
    • 1
    • 2
  • Barry S. Komm
    • 1
    • 2
  1. 1.Department of Obstetrics and GynecologyUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Division of Reproductive BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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