Decidual Cell Function: Evidence for A Role in the Regulation of Serum CBG and A 60Kda Protein During Early Pregnancy in the Hamster

  • Wendell W. Leavitt
  • Sabine Rundle
  • Kevin Thompson
  • Kyle W. Selcer
  • Gary O. Gray
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 230)


Several serum proteins increase in titer during pregnancy. We tested the hypothesis that decidual cells may signal the production of certain serum proteins in the hamster. Measurement of serum CBG by equilibrium binding using either [3H]-progesterone or [3H]-Cortisol in conjection with ion exchange chromatography showed that decidualization increased CBG levels. Two-dimensional gel electrophoresis revealed that a 60Kda protein increases markedly in the serum of the hormonally pseudopregnant (PSP) animal soon after artificial induction of decidualization on PSP day 4. The 60Kda serum protein remains low in the nondecidualized PSP animal, but it increases in the pregnant gnimal. A photoaffinity labeling procedure was used to covalently bind [3H]-androstadienolone to CBG. Fluorography of 2D gels run under denaturing conditions established that the 60Kda protein did not bind steroid as did CBG (69Kda). To determine whether decidual cells could induce the 60Kda and CBG proteins, different numbers of decidual celjLs were injected IP into PSP recipients. A single injection of 50 x 10 decidual cells induced both serum proteins within 48h, whereas the same number of hamster fetal cells was ineffective. Thus, these results demonstrate that hamster decidual cells induce a 60Kda protein of unknown function and serum CBG. Since the decidual cell itself does not appear to be the source of either protein, it follows that the decidual cell signals the synthesis and secretion of these proteins elsewhere in the body, most likely in the liver. To our knowledge, this is the first demonstration that the decidual cell regulates serum CBG and other proteins in this manner.


Progesterone Receptor 60Kda Protein Decidual Cell Pregnancy Serum Hamster Embryo Cell 
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  1. 1.
    C. A. Finn and L. Martin, The control of implantation, J. Reprod. Fertil. 39: 195–206 (1974).PubMedCrossRefGoogle Scholar
  2. 2.
    M. G. Shelesnyak, A history of research on nidation, Ann. N.Y. Acad. Scil. 476: 5–24 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    E. B. Astwood, An assay method for progesterone based upon the decidual cell reaction in the rat, J. Endocrinol. 1: 49–55 (1939).CrossRefGoogle Scholar
  4. 4.
    M. J. K. Harper, Hormonal control of the deciduomal response of the golden hamster uterus, Anat. Rec. 167: 225–230 (1970).PubMedCrossRefGoogle Scholar
  5. 5.
    G. C. Blaha and W. W. Leavitt, Deciduomal responses in the uteri of ovariectomized golden hamster, comparing progesterone and three closely related Steroids applied in utero, Biol. Reprod. 18: 441–447 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    W. W. Leavitt, A. Takeda, and R. G. MacDonald, Progesterone regulation of protein synthesis and steroid receptor levels in decidual cells, Ann. N.Y. Acad. Scil. 476: 136–157 (1986).PubMedCrossRefGoogle Scholar
  7. 7.
    B. R. Rao and W. G. Weist, Receptors for progesterone, Gynecol. Oncol. 2: 239–249 (1974).PubMedCrossRefGoogle Scholar
  8. 8.
    J. R. Reel, S. D. Van Dewark, Y. Shih, and M. R. Calantine, Macromolecular binding and metabolism of progesterone in the decidual and pseudopregnant rat and rabbit uterus, Steroids 18: 491–502 (1971).CrossRefGoogle Scholar
  9. 9.
    Y. S. Do and W. W. Leavitt, Characterization of a specific progesterone receptor in decidualized hamster uterus, Endocrinology 102: 443–451 (1978).PubMedCrossRefGoogle Scholar
  10. 10.
    T. C. Spelsberg, B. A. Littlefield, R. Seelke, G. N. Dani, H, Toyoda, P. Boyd-Leinen, C. Thrall, and O. L. Kon, Role of specific chromosomal proteins and DNA sequences in the nuclear binding sites for steroid receptors, Rec. Prog. Horm. Res. 39: 463–513 (1983).PubMedGoogle Scholar
  11. 11.
    W. W. Leavitt, T. J. Chen, Y. S. Do, B. D. Carlton, and T. C. Allen, Biology of progesterone receptors, Chapter 7, in: “Receptors and Hormone Action,” Volume 2, B. W. O’Malley and L, Birnbaumer, eds., Academic Press, New York, pp. 157–188 (1978).Google Scholar
  12. 12.
    R. G. MacDonald, K. O. Morency, and W. W. Leavitt, Progesterone modulation of specific protein synthesis in the decidualized hamster uterus, Biol. Reprod. 28: 753–766 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    W. W. Leavitt, R. G. MacDonald, and G. T. Shwaery, Characterization of deciduoma marker proteins in hamster uterus: detection in decidual cell cultures, Biol. Reprod. 32: 631–643 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    B. W. O’Malley, D. R. Roop, E. C. Lai, J. L. Nordstrom, J. F. Catterall, G. E. Swaneck, D. A. Colbert, M. J. Tsai, A. Dugaiczyk, and S. L. C. Woo, The ovalbumin gene: Organization, structure, transcription and regulation, Rec. Prog. Horm. Res. 35: 1–46 (1979).PubMedGoogle Scholar
  15. 15.
    P. Chambon, A. Dierich, M. Gaub, S. Jokowler, J. Jongstra, A. Krust, J. LePennec, P. Oudet, and T. Reudelhuber, Promoter elements of genes coding for proteins and modulation of transcription by estrogen and progesterone, Rec. Prog. Horm. Res. 40: 1–42 (1984).PubMedGoogle Scholar
  16. 16.
    J. Hau (ed.), “Pregnancy Proteins in Animals,” Walter de Gruyter, Berlin, 536 pp. (1986).Google Scholar
  17. 17.
    G. O. Gray, S. Rundle, and W. W. Leavitt, Purification and partial characterization of a corticosteroid-binding globulin from hamster serum, Biochim. Biophys. Acta 926: 40–53 (1987).PubMedCrossRefGoogle Scholar
  18. 18.
    M. N. Berry and D. S. Friend, High-yield preparation of isolated rat liver parenchymal cells. A biochemical and fine structural study, J. Cell Biol. 43: 506–520 (1969).PubMedCrossRefGoogle Scholar
  19. 19.
    R. Enat, D. M. Jefferson, N. Ruiz-Opazo, Z. Gatmaitany, L. A. Leinwand, and L. M. Reid, Hepatocyte proliferation in vitro: Its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix, Proc. Natl. Acad. Sci. USA 81: 1411–1415 (1984).PubMedCrossRefGoogle Scholar
  20. 20.
    W. W. Leavitt, T. J. Chen, and R. W. Evans, Regulation and function of estrogen and progesterone receptor systems, in: “Steroid Hormone Receptor Systems,” W. W. Leavitt and J. H. Clark, eds., Plenum Press, New York, pp. 197–222 (1979).Google Scholar
  21. 21.
    W. W. Leavitt, D. O. Toft, C. A. Strott, and B. W. O’Malley, A specific progesterone receptor in the hamster uterus: Physiologic properties and regulation during the estrous cycle, Endocrinology 94: 1041–1053 (1974).PubMedCrossRefGoogle Scholar
  22. 22.
    U. S. Seal and R. P. Doe, Vertebrate distribution of corticosteroid binding globulin and some endocrine effects on concentration, Steroids 5: 827–41 (1965).CrossRefGoogle Scholar
  23. 23.
    D. J. Hryb, M. S. Khan, N. A. Romas, and W. Rosner, Specific binding of human corticosteroid-binding globulin to cell membranes, Proc. Natl. Acad. Sci. USA 83: 3253–56 (1986).PubMedCrossRefGoogle Scholar
  24. 24.
    P. K. Siiteri, J. T. Murai, G. L. Hammond, J. A. Nisker, W. J. Raymoure, and R. W. Kuhn, The serum transport of steroid hormones,.. Rec. Prog. Horm. Res. 38: 457–510 (1982).PubMedGoogle Scholar
  25. 25.
    M. Perrot-Applanat, O. Racadot, and E. Milgrom, Specific localization of plasma corticosteroid-binding globulin immunoreactivity in pituitary corticotrophs, Endocrinology 115: 559–69 (1984).PubMedCrossRefGoogle Scholar
  26. 26.
    R. W. Kuhn, A. L. Green, W. J. Raymoure, and P. K. Siiteri, Immunocytochemical localization of corticosteroid-binding globulin in rat tissues, J. Endocrinol. 108: 31–36 (1986).PubMedCrossRefGoogle Scholar
  27. 27.
    A. A. Sandberg and W. R. Slaunwhite, Jr., Transcortin: a corticosteroid binding protein of plasma. II. Levels in various conditions and the effect of estrogens, J. Clin. Invest. 38: 1290–98 (1959).PubMedCrossRefGoogle Scholar
  28. 28.
    H. E. Rosenthal, W. R. Slaunwhite, Jr., and A. A. Sandberg, Transcortin: a corticosteroid-binding protein of plasma. XI. Effects of estrogens on pregnancy in guinea pigs, Endocrinology 85: 825–30 (1969).PubMedCrossRefGoogle Scholar
  29. 29.
    J. L. Gueriguian, M. E. Sawyer, and W. H. Pearlman, A comparative study of progesterone and cortisol-binding activity in the uterus and serum of pregnant and non-pregnant women, J. Endocrinol 61: 331–45 (1974).PubMedCrossRefGoogle Scholar
  30. 30.
    C. L. Coe, J. T. Murai, S. G. Wiener, S. Levine, and P. K. Siiteri, Rapid Cortisol and corticosteroid-binding globulin responses during pregnancy and after estrogen administration in the squirrel monkey, Endocrinology 118: 435–40 (1986).PubMedCrossRefGoogle Scholar
  31. 31.
    L. Savu, E. A. Nunez, and M. F. Jayle, High affinity testosterone, corticosterone and progesterone binding activities in pregnant hamster serum, Endocrinology 101: 369–77 (1977).PubMedCrossRefGoogle Scholar
  32. 32.
    J. W. Weiser, Y. S. Do, and D. Feldman, Synthesis and secretion of corticosteroid-binding globulin by rat liver. A source of heterogeneity of hepatic corticosteroid-binders, J. Clin. Invest. 63: 461–67 (1979).PubMedCrossRefGoogle Scholar
  33. 33.
    M. S. Khan, D. Aden, and W. Rosner, Human corticosteroid binding globulin is secreted by a hepatoma-derived cell line, J. Steroid Biochem. 20: 677–78 (1984).PubMedCrossRefGoogle Scholar
  34. 34.
    A. Takeda and W. W. Leavitt, Temporal effects of progesterone domination on estrogen and oxytocin receptors in hamster uterus, J. Steroid Biochem. 25: 219–224 (1986).PubMedCrossRefGoogle Scholar
  35. 35.
    K. W. Selcer and W. W. Leavitt, Hamster uterine tissue concentrates CBG during decidualization, Program of Annual Meeting of Endocrine Society, p. 105 (1987).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Wendell W. Leavitt
    • 1
  • Sabine Rundle
    • 1
  • Kevin Thompson
    • 1
  • Kyle W. Selcer
    • 1
  • Gary O. Gray
    • 1
  1. 1.Department of BiochemistryTexas Tech University Health Sciences CenterLubbockUSA

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