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Methodological Approaches in Studies of Altered Mitochondrial and Peroxisomal β-Oxidation

  • Chapter
Cells, Membranes, and Disease, Including Renal

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 17))

Abstract

The study of Mt and Px β-oxidation (which diseases or xenobiotics may affect) needs proper methodology. This is considered, for liver and other samples including biopsies, under the following heads:

  • Substrate preparation (incl. synthesis of acylCn’s and acylCoA’s).

  • Preparation and use of Mt fractions (incl. ‘couplina’; media).

Refs. list abridged by Editor, and abbreviations introduced:

Mt, mitochondria(l); Px, perixosome(s)/peroxisomal; de’ase, dehydrogenase (but LDH if lactate); tr’ase, transferase; synthase ≡ synthetase.

Cn, carnitine; FA, fatty acid; FFA, free FA; PL, phospholipid(s); py, palmitoyl (hence ‘palmitoyl-CoA ester’ in MS. has become pyCoA); BHT, butylated hydroxytoluene; BSA, bovine serum albumin; DTT, dithiothreitol; PCA, perchloric acid; RCR/RCI, respiratory control ratio/index; s.a., specific (radio)activity; THF, tetrahydrofuran.

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Author information

Authors and Affiliations

  1. Institute of Physiology and Biochemistry, Dental School, Blindern University of Oslo, Oslo 3, Norway

    Harald Osmundsen

  2. Department of Child Health and Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, NE2 4HH, Newcastle upon Tyne, UK

    Kim Bartlett

  3. Department of Pharmacological Sciences, Medical School, University of Newcastle upon Tyne, NE2 4HH, Newcastle upon Tyne, UK

    H. Stanley A. Sherratt

Authors
  1. Harald Osmundsen
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  2. Kim Bartlett
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  3. H. Stanley A. Sherratt
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, 72 The Chase, GU2 5UL, Guildford, UK

    Eric Reid

  2. University of Cambridge, Cambridge, UK

    G. M. W. Cook  & J. P. Luzio  & 

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© 1987 Plenum Press, New York

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Osmundsen, H., Bartlett, K., Sherratt, H.S.A. (1987). Methodological Approaches in Studies of Altered Mitochondrial and Peroxisomal β-Oxidation. In: Reid, E., Cook, G.M.W., Luzio, J.P. (eds) Cells, Membranes, and Disease, Including Renal. Methodological Surveys in Biochemistry and Analysis, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1283-3_4

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  • DOI: https://doi.org/10.1007/978-1-4684-1283-3_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1285-7

  • Online ISBN: 978-1-4684-1283-3

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