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Receptor-Mediated Endocytosis in Rat Liver Parenchymal and Non-Parenchymal Cells, Studied by Means of Subcellular Fractionation in Density Gradients

  • Chapter
Cells, Membranes, and Disease, Including Renal

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 17))

Abstract

In order to evaluate the influence of cellular derangements on hepatic endocytosis, its pathways have to be elucidated. Ways are now described to separate different cell types concerned, and to follow the endocytosis of different labelled ligands by subcellular fractionation techniques employing sucrose, Nycodenz or Percoll density gradients. The fractions were analyzed for degraded (acidsoluble) and undegraded ligands and for relevant marker enzymes (more meaningful because of the use of purified cell types). Different cell types differed in the intracellular processing of the ligands. Endocytosis has been studied in cultures also. The steps seem to be: → small, then larger, endosomes → (degradation) lysosome progression.

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Author information

Authors and Affiliations

  1. Institute for Nutrition Research School of Medicine, University of Oslo, P.O. Box 1046, 0316, Oslo 3, Norway

    G. M. Kindberg, W. Eskild, K-J. Andersen, K. R. Norum & T. Berg

  2. Section for Clinical Research and Molecular Medicine, University of Bergen, 5016, Haukeland Sykehus, Bergen, Norway

    K-J. Andersen

Authors
  1. G. M. Kindberg
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  2. W. Eskild
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  3. K-J. Andersen
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  4. K. R. Norum
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  5. T. Berg
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, 72 The Chase, GU2 5UL, Guildford, UK

    Eric Reid

  2. University of Cambridge, Cambridge, UK

    G. M. W. Cook  & J. P. Luzio  & 

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© 1987 Plenum Press, New York

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Kindberg, G.M., Eskild, W., Andersen, KJ., Norum, K.R., Berg, T. (1987). Receptor-Mediated Endocytosis in Rat Liver Parenchymal and Non-Parenchymal Cells, Studied by Means of Subcellular Fractionation in Density Gradients. In: Reid, E., Cook, G.M.W., Luzio, J.P. (eds) Cells, Membranes, and Disease, Including Renal. Methodological Surveys in Biochemistry and Analysis, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1283-3_33

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  • DOI: https://doi.org/10.1007/978-1-4684-1283-3_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1285-7

  • Online ISBN: 978-1-4684-1283-3

  • eBook Packages: Springer Book Archive

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