Skip to main content
SpringerLink
Log in

Gastroduodenal Epithelial Cells: The Role of the Apical Membrane in Mucosal Protection

  • Chapter
Cells, Membranes, and Disease, Including Renal

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 17))

Abstract

The aim was to investigate apical membranes of the epithelial cells in the upper GI* tract as a possible barrier to luminal acid and other insults, forming part of the ‘intrinsic protective mechanism’ of the mucosa. To study the integrity and transport capabilities of membrane vesicles, p.m.’s were isolated from duodenal enterocytes (furnishing BBMV’s) and histamine-stimulated parietal cells (furnishing SAV’s) and, for comparison, microsomes from resting parietal cells. The effect of trypsin on membrane vesicle integrity and function was determined. Both BBMV’s and SAV’s manifested a major population of vesicles resistant to trypsin.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Davenport, H.W. (1975) Mayo Clin. Proc. 50, 507–514.

    PubMed  CAS  Google Scholar 

  2. Schwartz, K. (1910) Beitrage Klin. Chirurg. 67, 96–128.

    Google Scholar 

  3. Powell, D.W. (1984) in Mechanisms of Mucosal Protection in the Upper Gastrointestinal Tract (Allen, A., Flemström, G., Gorner, A., Silen, W. & Turnberg, L.A., eds.), Raven Press, New York, pp. 1–6.

    Google Scholar 

  4. Guteknecht, J. & Tosteson, D.C. (1970) J. Gen. Physiol. 55, 359–374.

    Article  Google Scholar 

  5. Helenius, A. & Simons, K. (1975) Biochim. Biophys. Acta 415, 29–79.

    PubMed  CAS  Google Scholar 

  6. McLaughlin, S.G. & Dilger, J.P. (1980) Physiol. Rev. 60, 825–863.

    PubMed  CAS  Google Scholar 

  7. Olender, E.J., Fromm, D., Furukawa, T. & Kolis, M. (1984) Gastroenterology 86, 698–705.

    PubMed  CAS  Google Scholar 

  8. Wolosin, M. & Forte, J.G. (1981) J. Biol. Chem. 256, 3149–3152.

    PubMed  CAS  Google Scholar 

  9. Kessler, M., Acuto, O., Storelli, C., Murer, H., Müller, M. & Semenza, G. (1978) Biochim. Biophys. Acta 506, 136–154.

    Article  PubMed  CAS  Google Scholar 

  10. Bradford, M. (1976) Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  11. Peters, T.J. (1976) Clin, Sci. 51, 557–574.

    CAS  Google Scholar 

  12. Hirst, B.H. & Forte, J.G. (1985) Biochem. J. 231, 641–649.

    PubMed  CAS  Google Scholar 

  13. Black, J.A., Forte, J.G. & Hirst, B.H. (1985) J. Physiol. 371, 136P.

    Google Scholar 

  14. Hopfer, U., Nelson, K., Perrotto, J. & Isselbacher, K.J. (1973) J. Biol. Chem. 248, 25–32.

    PubMed  CAS  Google Scholar 

  15. Shinitzky, M. & Barenholz, Y. (1978) Biochim. Biophys. Acta 515, 367–394.

    PubMed  CAS  Google Scholar 

  16. Rabon, E., Takeguchi, N. & Sachs, G. (1980) J. Memb. Biol. 53, 109–117.

    Article  CAS  Google Scholar 

  17. Ives, H.E. & Verkman, A.S. (1985) Am. J. Physiol. 249, F933- F940.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, NE2 4HH, Newcastle upon Tyne, UK

    J. M. Wilkes, H. J. Ballard, J. A. E. Latham & B. H. Hirst

Authors
  1. J. M. Wilkes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Ballard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. A. E. Latham
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. H. Hirst
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Guildford Academic Associates, 72 The Chase, GU2 5UL, Guildford, UK

    Eric Reid

  2. University of Cambridge, Cambridge, UK

    G. M. W. Cook  & J. P. Luzio  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Plenum Press, New York

About this chapter

Cite this chapter

Wilkes, J.M., Ballard, H.J., Latham, J.A.E., Hirst, B.H. (1987). Gastroduodenal Epithelial Cells: The Role of the Apical Membrane in Mucosal Protection. In: Reid, E., Cook, G.M.W., Luzio, J.P. (eds) Cells, Membranes, and Disease, Including Renal. Methodological Surveys in Biochemistry and Analysis, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1283-3_25

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-1283-3_25

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1285-7

  • Online ISBN: 978-1-4684-1283-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation