Abstract
ApoE* is a glycoprotein of 299 amino acids which is associated primarily with VLDL and HDL in human plasma (1,2). It is a polymorphic protein with three common alleles inherited in a co-dominant fashion (3,4), the most common allele coding for apoE3. Homozygosity for this allele is present in approximately 60% of normal subjects, and this is considered to be the normal phenotype (5). Two other common alleles, which code for apoE2 and apoE4, differ from apoE3 by only one amino acid each (6). The allelic frequency of each of these two alleles is approximately 10–15%. Homozygosity for the apoE2 allele is associated with type III hyperlipoproteinemia (3,4) while apoE4 is associated with type V hyperlipoproteinemia (7). Previous investigations have demonstrated that apoE2 is catabolized abnormally slowly in vivo (8) and has a reduced binding affinity for cellular receptors (9). ApoE4 has a normal cellular receptor binding activity when compared to apoE3 (9). The results of these and other studies have led to the proposal that apoE has a major role in modulating the catabolism of remnants of triglyceride-rich lipoprotein particles from the intestine.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C.B. Blum, L. Aron, and R. Sciacca. Radioimmunoassay studies on human apolipoprotein E. J Clin Invest 66:1240 (1980).
R.J. Havel, L. Kotite, J.L. Vigne, J.P. Kane, P. Tun, N. Phillips, and G.C. Chen. Radioimmunoassay of human arginine-rich apolipoprotein, apolipoprotein E. J Clin Invest 66:1351 (1980).
G. Utermann, M. Jaeschke, and J. Menzel. Familial hyperlipoproteinemia type III: deficiency of a specific apolipoprotein (apoE-III) in the very low density lipoproteins. FEBS Lett 56:352 (1975).
V.I. Zannis, and J.L. Breslow. Human very low density lipoprotein apolipoprotein E isoprotein polymorphism is explained by genetic variation and post-translational modification. Biochemistry 20:1033 (1981).
G. Ghiselli, R.E. Gregg, L.A. Zech, E.J. Schaefer, and H.B. Brewer, Jr. Phenotype study of apolipoprotein E isoforms in hyperlipoproteinemia patients. Lancet ii:405 (1982).
S.C. Rall, Jr., K.H. Weisgraber, and R.W. Mahley. Human apolipoprotein E. The complete amino acid sequence. J Biol Chem 257:4171 (1982).
G. Ghiselli, E.J. Schaefer, L.A. Zech, R.E. Gregg, and H.B. Brewer, Jr. Increased prevalence of apolipoprotein E4 in type V hyperlipoproteinemia. J Clin Invest 70:474 (1982).
R.E. Gregg, L.A. Zech, E.J. Schaefer, and H.B. Brewer, Jr. Type III hyperlipoproteinemia: defective metabolism of an abnormal apolipoprotein E. Science 211:584 (1981).
K. H. Weisgraber, T.L. Innerarity, and R.W. Mahley. Abnormal lipoprotein receptor-binding activity of the human E apoprotein due to cysteine-arginine interchange at a single site. J Biol Chem 257:2518 (1982).
J.L. Goldstein, M.S. Brown, in The Metabolic Basis of InheritedDisease, J.B. Stanbury, J.B. Wyngaarden, D.S. Frederickson, J.L. Goldstein, and M.S. Brown, Eds., McGraw-Hill, New York, N.Y. pp. 672–712 (1983).
G. Utermann, K.H. Vogelberg, A. Steinmetz, W. Schoenborn, N. Pruin, M. Jaeschke, M. Hees. Polymorphism of apolipoprotein E. II. Genetics of hyperlipoproteinemia type III. Clin Genet 15:37 (1979).
H.-J. Menzel, R.-G. Kladetzky, G. Assmann. Apolipoprotein E polymorphism and coronary artery disease. Arteriosclerosis 3:310 (1983).
D. Bouthillier, C.F. Sing, and J. Davignon. Apolipoprotein E phenotyping with a single gel method: Application to the study of informative matings. J Lipid Res 24:1060 (1983).
G. Utermann, I. Kindermann, H. Kaffarnik, and A. Steinmetz. Apolipoprotein E phenotypes and hyperlipidemia. Hum Genet 65:232 (1984).
G. Assmann, G. Schmitz, H.-J. Menzel, and H. Schulte. Apolipoprotein E polymorphism and hyperlipidemia. Clin Chem 30:641 (1984).
C. F. Sing, and J. Davignon. Role of the apolipoprotein E polymorphism in determining normal plasma lipid and lipoprotein variation. Am J Hum Genet 37:268 (1985).
H.B. Brewer, Jr., L.A. Zech, R.E. Gregg, D.S. Schwartz, and E.J. Schaefer. Type III hyperlipoproteinemia: diagnosis, molecular defects, pathology, and treatment. Ann Intern Med 98:623 (1983).
R.E. Gregg, R. Ronan, L.A. Zech, G. Ghiselli, E.J. Schaefer, and H.B. Brewer, Jr. Abnormal metabolism of apolipoprotein E4. Arteriosclerosis 2:420a (1982).
R.E. Gregg, L.A. Zech, E.J. Schaefer, and H.B. Brewer, Jr. Apolipoprotein E metabolism in normolipoproteinemic human subjects. J Lipid Res 25:1167 (1984).
R.E. Gregg, E.J. Schaefer, L.A. Zech, H.B. Brewer, Jr. Lipoprotein distribution of radioiodinated type III and type V apolipoprotein E (apoE). Arteriosclerosis 1:85 (1981).
R.E. Gregg, L.A. Zech, C. Gabelli, D. Stark, D. Wilson, H.B. Brewer, Jr. LDL catabolism in normolipidemic apoE2 homozygotes. Circulation 70, Supp 11:312 (1984).
J.P. Kane. Apolipoprotein B.: structure and metabolic heterogeneity. Ann Rev Physiol 45:632 (1983).
G. Ghiselli, E.J. Schaefer, P. Gascon, and H.B. Brewer, Jr. Type III hyperlipoproteinemia associated with plasma apolipoprotein E deficiency. Science 214:1239 (1981).
C. Gabelli, D.G. Stark, R.E. Gregg, H.B. Brewer, Jr. Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. J Lipid Res (in press).
E.J. Schaefer, L.A. Zech, G. Ghiselli, H.B. Brewer, Jr. ApoB-48 metabolism: retarded catabolism due to apoE deficiency. Arteriosclerosis 2:424a (1982).
C. Gabelli, R.E. Gregg, L.A. Zech, J.M. Hoeg, H.B. Brewer, Jr. ApoB-48 catabolism in normal and homozygous familial hypercholesterolemic individuals. Clin Res 32:116a (1984).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer Science+Business Media New York
About this chapter
Cite this chapter
Gregg, R.E., Bryan Brewer, H. (1986). The Role of Apolipoprotein E in Modulating the Metabolism of Apolipoprotein B-48 and Apolipoprotein B-100 Containing Lipoproteins in Humans. In: Angel, A., Frohlich, J. (eds) Lipoprotein Deficiency Syndromes. Advances in Experimetal Medicine and Biology, vol 201. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1262-8_25
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1262-8_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1264-2
Online ISBN: 978-1-4684-1262-8
eBook Packages: Springer Book Archive