Metabolic Interrelationships of HDL Subclasses
Levels of plasma HDL are determined by a complex array of metabolic processes involving synthesis, transfer, recycling, and tissue uptake of individual apoprotein and lipid components1. At equilibrium, these components are organized within a variety of particulate forms, and detailed understanding of HDL metabolism requires consideration of factors responsible for this macromolecular heterogeneity. The first description of the HDL particle spectrum identified three major forms of HDL based on flotation rates (F°1.20) in the analytic ultracentrifuge2: HDL1, of F°1.20>9 and density overlapping with LDL; HDL2, of F°1.20 3.5–9 (d 1.063–1.125 g/ml), and HDL3, of F°1.200–3.5 (d 1.125–1.200 g/ml). Figure 1 shows the analytic ultracentrifuge pattern of HDL2 and HDL3 in a representative normal subject, as well as the HDL profile as revealed by zonal ultracentrifugation3 and electrophoresis of HDL in native polyaerylamide gradient gels4. Each of these procedures has revealed further heterogeneity within the HDL2 and HDL3 density subclasses. In the case of gradient gel electrophoresis, heterogeneity is manifest as multiple electrophoretic bands which have been shown to represent distinct HDL subspecies of differing particle size.
KeywordsImmunoaffinity Chromatography Plasma High Density Lipoprotein High Density Lipo Fractional Catabolic Rate Flotation Rate
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