Abstract
In 1975, Paglia and Valentine (1) demonstrated the existence in human erythrocytes of a specific pyrimidine 5′-nucleotidase, which they found inactive on purine nucleotides. A deficiency of this enzyme is known which provokes an hemolytic anemia (reviewed in 2). In accordance with the reported enzymic specificity, the erythrocytes of these patients have markedly elevated concentrations of cytidine and uridine nucleotides but not of purine nucleotides. Although it is evident that dephosphorylation of AMP and IMP should occur in erythrocytes (see Bontemps et al., this volume), the enzyme(s) catalysing this process have hitherto not been identified. This report describes the partial purification and the kinetic properties of a purine-specific 5′-nucleotidase present in human erythrocytes.
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© 1986 Plenum Press, New York
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Bontemps, F., Van den Berghe, G., Hers, H.G. (1986). Identification of a Purine 5′-Nucleotidase in Human Erythrocytes. In: Nyhan, W.L., Thompson, L.F., Watts, R.W.E. (eds) Purine and Pyrimidine Metabolism in Man V. Advances in Experimental Medicine and Biology, vol 195B. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-1248-2_45
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DOI: https://doi.org/10.1007/978-1-4684-1248-2_45
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