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The Biosynthesis of Deoxyguanosine Triphosphate in Herpes Simplex Type-1 Infected Vero Cells Treated with Acyclovir and Hydroxyurea

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Purine and Pyrimidine Metabolism in Man V

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 195B))

Abstract

Herpes simplex virus type 1 (HSV-1) infection of cells causes induction of virally specified thymidine kinase, DNA polymerase, ribonucleotide reductase and deoxyribonuclease1-3. Also accompanying infection is a rise in deoxyribonucleoside triphosphates (dNTPs), particularly dTTP2,4. Acyclovir (ACV) is an acyclic deoxyguanosine analog which, in its triphosphate form, inhibits viral DNA polymerase5,6. ACV treatment dramatically increased the pool sizes of dATP and dGTP in infected Vero cells compared to infected untreated cells4. The source of these elevated purine deoxyribonucleotide pools could be from either enhanced de novo synthesis, salvage of deoxyribonucleosides derived from host cell DNA, or possibly a combination of the two pathways.

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References

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Authors and Affiliations

  1. Wellcome Research Laboratories, Research Triangle Park, N.C., 27709, USA

    Catherine U. Lambe, Donald J. Nelson & Phillip A. Furman

Authors
  1. Catherine U. Lambe
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  2. Donald J. Nelson
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  3. Phillip A. Furman
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Editor information

Editors and Affiliations

  1. University of California, San Diego La Jolla, California, USA

    W. L. Nyhan

  2. Scripps Clinic and Research Foundation, La Jolla, California, USA

    L. F. Thompson

  3. M. R. C. Clinical Research Center, Harrow, UK

    R. W. E. Watts

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© 1986 Plenum Press, New York

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Lambe, C.U., Nelson, D.J., Furman, P.A. (1986). The Biosynthesis of Deoxyguanosine Triphosphate in Herpes Simplex Type-1 Infected Vero Cells Treated with Acyclovir and Hydroxyurea. In: Nyhan, W.L., Thompson, L.F., Watts, R.W.E. (eds) Purine and Pyrimidine Metabolism in Man V. Advances in Experimental Medicine and Biology, vol 195B. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-1248-2_23

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  • DOI: https://doi.org/10.1007/978-1-4684-1248-2_23

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-1250-5

  • Online ISBN: 978-1-4684-1248-2

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