Abstract
Academic and industrial interests have recently motivated a rapid development of streptomycete recombinant DNA technology (9, 15, 25). This was made possible by the availability of plasmids and the critical discovery by Bibb et al. that plasmid DNA could be introduced to streptomycete protoplasts following polyethylene-glycol treatment (1). Since then, interspecific genomic cloning experiments have allowed the isolation of streptomycete antibiotic resistance genes which are normally associated with the production of antibiotics and the tyrosinase gene which catalyzes melanine synthesis (2, 13, 16, 20, 23). These genes have been used to construct vectors from plasmid and bacteriophage replicons (5). Here we will focus primarily on the development of the vectors most commonly used today which were derived from the streptomycete plasmids SLP1 and pIJ101.
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© 1985 Plenum Press, New York
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Thompson, C.J., Davies, J.E. (1985). Streptomycete Plasmid Cloning Vectors. In: Alaeddinoğlu, N.G., Demain, A.L., Lancini, G. (eds) Industrial Aspects of Biochemistry and Genetics. NATO ASI Series, vol 87. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1227-7_3
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DOI: https://doi.org/10.1007/978-1-4684-1227-7_3
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