Advertisement

Application of Field Desorption and Secondary Ion Mass Spectrometry for Glycolipid Analysis

  • Shizuo Handa
  • Yasunori Kushi
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)

Abstract

Glycolipids are well known cell membrane components and play a role in several biological phenomena on the cell surface.1 As glycolipids are relatively minor components of cells, it is necessary to develop analytical methods for small amounts of samples. Mass spectrometry have proved to be powerful tools for this purpose. Electron impact and chemical ionization mass spectrometry provide good information on the structure of permethylated or permethylated and reduced glycolipids.2–4 However, these ionization methods require the derivatization of the sample, and the ion intensities of the high mass region are very weak and difficult to detect continuously during analysis. The recently introduced “soft ionization techniques” are successfully applied for the analyses of non-volatile and thermolabile compounds. These include field desorption (FD), fast atom bombardment (FAB) and secondary ion mass spectrometry (SI-MS). However only a few reports on the application of these new techniques for the analysis of glycolipids5–11 have been published.

Keywords

Fast Atom Bombardment Glyceryl Ether Fast Atom Bombardment Mass Spectrometry High Performance Liquid Chromatogra Field Desorption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Makita, S. Handa, T. Taketomi, and Y. Nagai, eds., “New Vistas in Glycolipid Research,” Plenum Publishing Corp., New York (1982).Google Scholar
  2. 2.
    R. W. Ledeen, S. K. Kundu, H. C. Price, and J. W. Fong, Mass spectra of permethyl derivatives of glycosphingolipids, Chem. Phys. Lipid 13:429 (1974).CrossRefGoogle Scholar
  3. 3.
    K.-A. Karlsson, I. Pascher, W. Pimlott, and B. E. Samuelsson, Use of mass spectrometry for the carbohydrate composition and sequence analysis of glycosphingolipids, Biomed. Mass Spectrom 1:49 (1974).PubMedCrossRefGoogle Scholar
  4. 4.
    T. Ariga, R. K. Yu, M. Suzuki, S. Ando, and T. Miyatake, Characterization of GM1 ganglioside by direct inlet chemical ionization mass spectrometry, J. Lipid Res. 23:437 (1982).PubMedGoogle Scholar
  5. 5.
    C. E. Costello, B. W. Wilson, K. Biemann, and J. V. Reinhold, Analysis of glycosphingolipids by field desorption mass spectrometry, in: “Cell Surface Glycolipids,” C. C. Sweeley, ed., ACS Symp. Series 128 p. 36 (1980).Google Scholar
  6. 6.
    Y. Kushi and S. Handa, Application of field desorption mass spectrometry for the analysis of sphingoglycolipids, J. Biochem. 91:923 (1982).PubMedGoogle Scholar
  7. 7.
    S. Handa and Y. Kushi, High performance liquid chromatography and structural analysis by field desorption mass spectrometry of underivatized glycolipid, in: “New Vistas in Glycolipid Research,” A. Makita, T. Taketomi, S. Handa, and Y. Nagai, eds., Plenum Publishing Corp., New York (1982).Google Scholar
  8. 8.
    H. Egge, J. Dabrowski, P. Hanfland, A. Dell, and U. Dabrowski, High resolution 1H nuclear magnetic resonance spectroscopy and “Soft ionization” mass spectrometry of glycosphingolipids, in: “New Vistas in Glycolipid Research,” A. Makita, T. Taketomi, S. Handa, and Y. Nagai, eds., Plenum Publishing Corp., New York (1982).Google Scholar
  9. 9.
    S. Handa, Y. Kushi, H. Kambara, and K. Shizukuishi, Secondary ion mass spectra of neutral sphingolipids, J. Biochem. 93:315 (1983).PubMedGoogle Scholar
  10. 10.
    M. Arita, M. Iwamori, T. Higuchi, and Y. Nagai, 1,1,3,3-Tetramethylurea and triethanolamine as a new useful matrix for fast atom bombardment mass spectrometry of gangliosides and neutral glycosphingolipids, J. Biochem. 93:319 (1983).PubMedGoogle Scholar
  11. 11.
    K. L. Rinehart, Fast atom bombardment mass spectrometry, Science 218:254 (1982).PubMedCrossRefGoogle Scholar
  12. 12.
    S. Hakomori, A rapid permethylation of glycolipids and polysaccharides catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide, J. Biochem. 55:205 (1964).PubMedGoogle Scholar
  13. 13.
    I. Ishizuka, M. Suzuki, and T. Yamakawa, Isolation and characterization of a novel sulfoglycolipids, “Seminolipid”, from boar testis and spermatozoa, J. Biochem. 73:77Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Shizuo Handa
    • 1
  • Yasunori Kushi
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineTokyo Medical and Dental UniversityYushima, Bunkyo-ku, Tokyo 113Japan

Personalised recommendations