Application of Field Desorption and Secondary Ion Mass Spectrometry for Glycolipid Analysis

  • Shizuo Handa
  • Yasunori Kushi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)


Glycolipids are well known cell membrane components and play a role in several biological phenomena on the cell surface.1 As glycolipids are relatively minor components of cells, it is necessary to develop analytical methods for small amounts of samples. Mass spectrometry have proved to be powerful tools for this purpose. Electron impact and chemical ionization mass spectrometry provide good information on the structure of permethylated or permethylated and reduced glycolipids.2–4 However, these ionization methods require the derivatization of the sample, and the ion intensities of the high mass region are very weak and difficult to detect continuously during analysis. The recently introduced “soft ionization techniques” are successfully applied for the analyses of non-volatile and thermolabile compounds. These include field desorption (FD), fast atom bombardment (FAB) and secondary ion mass spectrometry (SI-MS). However only a few reports on the application of these new techniques for the analysis of glycolipids5–11 have been published.


Fast Atom Bombardment Glyceryl Ether Fast Atom Bombardment Mass Spectrometry High Performance Liquid Chromatogra Field Desorption 
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    A. Makita, S. Handa, T. Taketomi, and Y. Nagai, eds., “New Vistas in Glycolipid Research,” Plenum Publishing Corp., New York (1982).Google Scholar
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    R. W. Ledeen, S. K. Kundu, H. C. Price, and J. W. Fong, Mass spectra of permethyl derivatives of glycosphingolipids, Chem. Phys. Lipid 13:429 (1974).CrossRefGoogle Scholar
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    C. E. Costello, B. W. Wilson, K. Biemann, and J. V. Reinhold, Analysis of glycosphingolipids by field desorption mass spectrometry, in: “Cell Surface Glycolipids,” C. C. Sweeley, ed., ACS Symp. Series 128 p. 36 (1980).Google Scholar
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    H. Egge, J. Dabrowski, P. Hanfland, A. Dell, and U. Dabrowski, High resolution 1H nuclear magnetic resonance spectroscopy and “Soft ionization” mass spectrometry of glycosphingolipids, in: “New Vistas in Glycolipid Research,” A. Makita, T. Taketomi, S. Handa, and Y. Nagai, eds., Plenum Publishing Corp., New York (1982).Google Scholar
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    S. Handa, Y. Kushi, H. Kambara, and K. Shizukuishi, Secondary ion mass spectra of neutral sphingolipids, J. Biochem. 93:315 (1983).PubMedGoogle Scholar
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    M. Arita, M. Iwamori, T. Higuchi, and Y. Nagai, 1,1,3,3-Tetramethylurea and triethanolamine as a new useful matrix for fast atom bombardment mass spectrometry of gangliosides and neutral glycosphingolipids, J. Biochem. 93:319 (1983).PubMedGoogle Scholar
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Shizuo Handa
    • 1
  • Yasunori Kushi
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineTokyo Medical and Dental UniversityYushima, Bunkyo-ku, Tokyo 113Japan

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