Comparative and Developmental Behavior of Alkali Labile Gangliosides in the Brain

  • R. Ghidoni
  • S. Sonnino
  • V. Chigorno
  • A. Malesci
  • G. Tettamanti
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)


In vertebrate tissues gangliosides are more abundant in brain than in other organs. About 50 different molecular species have been isolated and structurally characterized. The number of new gangliosides is rapidly increasing, due to the development of new technologies capable of detecting and characterizing very minor and less accessible components.


Sialic Acid Zebra Finch Brain Ganglioside Developmental Behavior Total Ganglioside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



GT1b containing 9-0-acetyl, N-acetylneurarainic acid


N-acetylneuraminic acid


GQ1b containing 9-0-acetyl,N-acetylneuraminic acid


thin layer chromatography


high performance thin layer chromatography


gas liquid chromatography


mass spectrometry


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  1. 1.
    V. Chigorno, S. Sonnino, R. Ghidoni, and G. Tettamanti, Densitometric quantification of brain gangliosides separated by two-dimensional thin layer chromatography, Neurochem. Intern, 4:397 (1982).CrossRefGoogle Scholar
  2. 2.
    S. Sonnino, R. Ghidoni, V. Chigorno, M. Masserini, and G. Tettamanti, Recognition by two-dimensional thin layer chromatography and densitometric quantification of alkali labile gangliosides from the brain of different animals, Anal. Biochem. 128:104 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    H. Wiegandt, Ganglioside, Ergeb. Physiol. Biol. Chem. Exp. Pharmacol. 57:190 (1966).Google Scholar
  4. 4.
    J. E. Evans and R. H. McCluer, Synthesis and characterization of sialosyllactosylceramide inner ester, Fed. Proc. 30:1133 (1971).Google Scholar
  5. 5.
    S. K. Gross, M. A. Williams, and R. H. McCluer, Alkali labile, sodium borohydride reducible ganglioside sialic acid residues in brain, Neurochem. 34:1351 (1980).CrossRefGoogle Scholar
  6. 6.
    J. Haverkamp, R. W. Veh, M. Sander, R. Schauer, J. P. Kamerling, and J. F. G. Vliegenthart, Demonstration of 9-0-acetyl, N-acetylneuraminic acid in brain gangliosides from various vertebrates including man, Hoppe-Seyler’s Z. Physiol. Chem. 358:1609 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    R. Ghidoni, S. Sonnino, G. Tettamanti, N. Baumann, G. Reuter, and R. Schauer, Isolation and characterization of a trisialoganglioside from mouse brain, containing 9-0-acetyl, N-acetylneuraminic acid, J. Biol. Chem. 255:6990 (1980).PubMedGoogle Scholar
  8. 8.
    V. Chigorno, S. Sonnino, R. Ghidoni, and G. Tettamanti, Isolation and characterization of a tetrasialoganglioside from mouse brain, containing 9-0-acetyl,N-acetylneuraminic acid, Neurochem. Intern 4:531 (1982).CrossRefGoogle Scholar
  9. 9.
    S. Hakomori and T. Saito, Isolation and characterization of a glycosphingolipid having a new sialic acid, Biochemistry 8:5082 (1969).PubMedCrossRefGoogle Scholar
  10. 10.
    G. Tettamanti, F. Bonali, S. Marchesini, and V. Zambotti, A new procedure for the extraction, purification and fractionation of brain gangliosides, Biochim. Biophys. Acta 296:160 (1973).PubMedGoogle Scholar
  11. 11.
    J. P. Kamerling, J. Haverkamp, J. F. G. Vliegenthart, C. Versluis, and R. Schauer, Mass spectrometry O-acetylated-N-acetylneuraminic acid, in: Recent development in mass spectrometry in biochemistry and medicine, Vol. 1, Plen. Press, New York, p. 503 (1978).CrossRefGoogle Scholar
  12. 12.
    M. Iwamori and Y. Nagai, A new chromatographic approach to the resolution of individual gangliosides, Biochim. Biophys. Acta 528:257 (1978).PubMedGoogle Scholar
  13. 13.
    H. Rahmann, Brain gangliosides and thermal adaption in vertebrates, Zool. Jb. Physiol. 85:209 (1981).Google Scholar
  14. 14.
    L. Svennerholm, The gangliosides, J. Lipid Res. 5:145 (1964).PubMedGoogle Scholar
  15. 15.
    L. Svennerholm, Quantitative estimation of sialic acid. II. A colorimetric resorcinol-hydrochloric acid method, Biochim. Biophys. Acta 24:604 (1957).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • R. Ghidoni
    • 1
  • S. Sonnino
    • 1
  • V. Chigorno
    • 1
  • A. Malesci
    • 1
  • G. Tettamanti
    • 1
  1. 1.Dept. of Biological ChemistryThe Medical School Univ. of MilanMilanItaly

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