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New Approaches in the Study of Ganglioside Metabolism

  • G. Tettamanti
  • R. Ghidoni
  • S. Sonnino
  • V. Chigorno
  • B. Venerando
  • A. Giuliani
  • A. Fiorilli
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)

Abstract

It is generally accepted that gangliosides are biosynthesized in the Golgi apparatus and then move to the plasma membrane which is the main site of their cellular location. 1–3 The process of ganglioside biosynthesis consists of sequential additions of monosaccharide units to a starting precursor (ceramide or psychosine) catalyzed by specific glycosyltransferases.1, 2, 4, 5 Gangliosides are degraded in the lysosomes, where several glycohydrolases remove sequentially the individual saccharide units and the formed ceramide is split by ceramidase into sphingosine and fatty acid.6–8 In the case of nerve cells the process of both biosynthesis and degradation of gangliosides is assumed to occur primarily in the perikaryon.7–9 Transport of gangliosides to and from the plasma membranes is mediated by fast axonal flow.9, 10

Keywords

Golgi Apparatus Specific Radioactivity High Performance Thin Layer Chromatography Liver Plasma Membrane Plasma Membrane Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DDQ

2,3-dichloro-5,6-dicyanobenzoquinone

TLC

thin layer chromatography

HPTLC

high performance thin layer chromatography

GLC

gas-liquid chromatography

HPLC

high performance liquid chromatography

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • G. Tettamanti
    • 1
  • R. Ghidoni
    • 1
  • S. Sonnino
    • 1
  • V. Chigorno
    • 1
  • B. Venerando
    • 1
  • A. Giuliani
    • 1
  • A. Fiorilli
    • 1
  1. 1.Dept. of Biological Chemistrythe Medical School University of MilanMilanItaly

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