New Approaches in the Study of Ganglioside Metabolism

  • G. Tettamanti
  • R. Ghidoni
  • S. Sonnino
  • V. Chigorno
  • B. Venerando
  • A. Giuliani
  • A. Fiorilli
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)


It is generally accepted that gangliosides are biosynthesized in the Golgi apparatus and then move to the plasma membrane which is the main site of their cellular location. 1–3 The process of ganglioside biosynthesis consists of sequential additions of monosaccharide units to a starting precursor (ceramide or psychosine) catalyzed by specific glycosyltransferases.1, 2, 4, 5 Gangliosides are degraded in the lysosomes, where several glycohydrolases remove sequentially the individual saccharide units and the formed ceramide is split by ceramidase into sphingosine and fatty acid.6–8 In the case of nerve cells the process of both biosynthesis and degradation of gangliosides is assumed to occur primarily in the perikaryon.7–9 Transport of gangliosides to and from the plasma membranes is mediated by fast axonal flow.9, 10


Golgi Apparatus Specific Radioactivity High Performance Thin Layer Chromatography Liver Plasma Membrane Plasma Membrane Fraction 
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thin layer chromatography


high performance thin layer chromatography


gas-liquid chromatography


high performance liquid chromatography


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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • G. Tettamanti
    • 1
  • R. Ghidoni
    • 1
  • S. Sonnino
    • 1
  • V. Chigorno
    • 1
  • B. Venerando
    • 1
  • A. Giuliani
    • 1
  • A. Fiorilli
    • 1
  1. 1.Dept. of Biological Chemistrythe Medical School University of MilanMilanItaly

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