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Biosynthesis In Vitro of Gangliosides Containing Gg- and Lc-Cores

  • Subhash Basu
  • Manju Basu
  • John W. Kyle
  • Hung-Che Chon
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)

Abstract

The structures of at least 40 different gangliosides containing N-acetylgalactosamine1–7 and N-acetylglucosamine8–21 have been reported by several laboratories, in addition to four hexosamine-free gangliosides (GM4, GM3, GD3 and GT3).22–26 All of these gangliosides are widely distributed in animal tissues, particularly in synaptic membranes and on erythrocyte surfaces. In general, the gangliosides may be reclassified as acidic glycosphingolipids (acidic GSLs) containing N-glycolyl-(NeuGc) or N-acetylneuraminic (NeuAc) moieties attached to two distinct oligosaccharide core structures. The gangliosides containing the gangliotetraosylceramide (GgOse4Cer; Gal βl-3GalNAcβ 1-4Galβl-4Glc-Cer) core appears to be ubiquitous in neuronal cells. However, extraneuronal tissues and erythrocytes of various species contain gangliosides with neo-lactotetraosylceramide (Galβ 1-4GlcNAcβ 1-3Galβ 1-4Glc-Cer; nLcOse4Cer) as the core structure (Fig. 1). At least three different galactosyl linkages (Galβ1-4Glc-R, Gal β1-3GalNAc-R′ and Gal β1-4GlcNAc-R″) (Fig. 2) and three different sialyl linkages (NeuAc α2-3Galβ 1-4Glc-R′, NeuAcα 2-8NeuAcα 2-3Gal-R″ and NeuAc α2-3Galβ 1-4GlcNAc-R‴) (Fig. 3) are present in gangliosides of the Gg- and Lc-series.

Keywords

Sialyltransferase Activity Buffy Coat Layer Galactosyltransferase Activity Embryonic Chicken Brain Acidic Glycosphingolipids 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Subhash Basu
    • 1
  • Manju Basu
    • 1
  • John W. Kyle
    • 1
  • Hung-Che Chon
    • 1
  1. 1.Department of Chemistry Biochemistry, Biophysics and Molecular Biology ProgramUniversity of Notre DameNotre DameUSA

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