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Ganglioside Distribution at Different Levels of Organization and its Biological Implications

  • Yoshitaka Nagai
  • Masao Iwamori
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 174)

Abstract

More than 100 molecular species of glycosphingolipids (GSL) having different carbohydrate chains have so far been isolated and characterized from various mammalian tissues and cells. They are classified into seven general types according to their asialo-carbohydrate structures (Table 1), which are biosynthesized via their own defined pathways. Although the systematic analysis of GSL on various cell surfaces, especially on erythrocyte membranes of various animal species, indicates that the biosynthetic pathways including the termination or modification of carbohydrate chain is under the regulation of genetic control, as shown in the case of blood group antigens, recent observations strongly indicate that the environmental or nongenomic information-dependent modification of carbohydrate chains including the induction of new biosynthetic pathways occurs at various cellular levels outside of the nuclei. Such dual nature of GSL imposes some difficulty when we attempt to identify the exact role of GSL in cell function. The knowledge of GSL distribution is helpful in recognizing the basic structure and the type of modification of the carbohydrate chain in morphologically or functionally specified cells in relation to cell function, differentiation, transformation and other cellular activities.

Keywords

Carbohydrate Chain Brain Ganglioside Extraneural Tissue Ganglioside Composition Individual Ganglioside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Y. Nagai and M. Iwamori, Brain and thymus gangliosides: Their molecular diversity and its biological implications and a dynamic annular model for their function in cell surface membranes, Mol. Cell. Biochem. 29:81 (1980).PubMedCrossRefGoogle Scholar
  2. 2.
    M. Iwamori and Y. Nagai, A new chromatographic approach to the resolution of individual gangliosides: ganglioside mapping, Biochim. Biophys. Acta 528:257 (1978).PubMedGoogle Scholar
  3. 3.
    C. C. Sweeley and B. Siddiqui, Chemistry of mammalian glycolipids, in: “The Glycoconjugates” Vol. II, M. L. Horowitz and W. Pigman, eds., Academic Press (1977).Google Scholar
  4. 4.
    G. Morgan, G. Gombos, and G. Tettamanti, Glycoproteins and glycolipids of the nervous system, in: “The Glycoconjugates” Vol. I, M. L. Horowitz and W. Pigman, eds., Academic Press (1977).Google Scholar
  5. 5.
    H. Wiegandt, The gangliosides, in: “Advances in Neurochemistry” Vol 4, B. W. Agranoff and M. H. Aprison, eds., Plenum Press (1982).Google Scholar
  6. 6.
    R. W. Ledeen, Gangliosides, in: “Handbook of Neurochemistry”, Vol 3 (2nd Ed.), A. Lajtha, ed., Plenum Press (1983).Google Scholar
  7. 7.
    T. Yamakawa, Glycolipids of mammalian red blood cells, in: “Lipoide”, E. Schutte, eds., Springer-Verlag, Berlin-Heidelberg-New York (1966).Google Scholar
  8. 8.
    M. Iwamori, J. Shimomura, S. Tsuyuhara, M. Mogi, S. Ishizaki, and Y. Nagai, Differential reactivities of fucosyl-GM1 and GM1 gangliosides on rat erythrocyte membrane revealed by the analysis with anti-fucosyl GM1 and GM1 antisera, J. Biochem. 94:1 (1983).PubMedGoogle Scholar
  9. 9.
    K. Ueno, Y. Kushi, C. Rokukawa, and S. Handa, Distribution of gangliosides in parenchymal and nonparenchymal cells of rat liver, Biochem. Biophys. Res. Commun. 105:681 (1982).PubMedCrossRefGoogle Scholar
  10. 10.
    E. H. Holms and S. Hakomori, Isolation and characterization of a new fucoganglioside accumulated in precancerous rat liver and in rat hepatoma induced by 2-N-acetyl aminofluorene, J. Biol. Chem. 257:7698 (1982).Google Scholar
  11. 11.
    T. Hirabayshi, T. Taki, M. Matsumoto, and K. Kojima, Comparative study on glycolipid composition between two cell types of rat ascite hepatoma cells, Biochim. Biophys. Acta 529:96 (1978).Google Scholar
  12. 12.
    M. Iwamori and Y. Nagai, Comparative study on ganglioside compositions of various rabbit tissues. Tissue-specificity in ganglioside molecular species of rabbit thymus, Biochim. Biophys. Acta 665:214 (1981).PubMedGoogle Scholar
  13. 13.
    R. W. Ledeen, R. K. Yu, and L. F. Eng, Gangliosides of human myelin: sialosylgalactosylceramide (G7) as a major component, J. Neurochem. 21:829 (1973).PubMedCrossRefGoogle Scholar
  14. 14.
    J. L. Magnani, D. F. Smith, and V. Ginsburg, Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms, Anal. Biochem. 109:399 (1980).PubMedCrossRefGoogle Scholar
  15. 15.
    M. Iwamori, M. Mogi, Y. Hirano, M. Nishio, H. Nakauchi, K. Okumura, and Y. Nagai, A quantitative analysis of cell surface glycosphingolipid with a fluorescence activated cell sorter, J. Immunol. Methods 57:381 (1983).PubMedCrossRefGoogle Scholar
  16. 16.
    S. Hakomori, Glycosphingolipids in cellular interaction, differentiation and oncogenesis, Ann. Rev. Biochem. 50:733 (1981).PubMedCrossRefGoogle Scholar
  17. 17.
    K. Sakakibara, T. Momoi, T. Uchida, and Y. Nagai, Evidence for association of glycosphingolipid with a colchicine-sensitive microtubule-like cytoskeletal structure of cultured cells, Nature 293:76 (1981).PubMedCrossRefGoogle Scholar
  18. 18.
    Y. Nagai and K. Sakakibara, Cytoskeleton-associated glycolipid (CAG) and its cell biological implications, in: “New Vistas in Glycolipid Research”, A. Makita, S. Handa, T. Taketomi, and Y. Nagai, eds., Plenum Press, New York (1982).Google Scholar
  19. 19.
    K. Sakakibara, M. Iwamori, T. Uchida, and Y. Nagai, Immunohistochemical localization of galactocerebroside in kidney, liver and lung of golden hamster, Experientia 37:712 (1981).PubMedCrossRefGoogle Scholar
  20. 20.
    C. H. Streuli, B. Patel, and D. R. Critchley, The cholera toxin receptor ganglioside GM1 remains associated with Triton X-100 cytoskeletons of BALB/c 3T3 cells, Exp. Cell Res. 136:247 (1981).PubMedCrossRefGoogle Scholar
  21. 21.
    D. M. Marcus and R. Janis, Localization of glycosphingolipids in human tissues by immunofluorescence, J. Immunol. 104:1530 (1970).PubMedGoogle Scholar
  22. 22.
    S. Ando, N.-C. Chang, and R. K. Yu, High-performance thin-layer chromatography and densitometric determination of brain ganglioside compositions of several species, Anal. Biochem. 89:437 (1978).PubMedCrossRefGoogle Scholar
  23. 23.
    K. Ueno, S. Ando, and R. K. Yu, Gangliosides of human, cat and rabbit spinal cords and cord myelin, J. Lipid Res. 19:863 (1978).PubMedGoogle Scholar
  24. 24.
    R. K. Yu and K. Igbal, Sialosylgalactosyl ceramide as a specific marker for human myelin and oligodendroglial perikarya: gangliosides of human myelin, oligodendroglia and neurons, J. Neurochem. 32:293 (1979).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Yoshitaka Nagai
    • 1
  • Masao Iwamori
    • 1
  1. 1.Department of Biochemistry Faculty of MedicineUniversity of TokyoBunkyo-ku, Tokyo 113Japan

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