Abstract
In 1956 Clark described a Polarographic electrode which allowed to measure the Po2, particularly in biological materials with an exactitude and stability which hitherto was not possible. This was achieved by a membrane which separated electrode and biological material from each other so that the Polarographic reaction at the Pt surface and the current transfer at the reference electrode took place in a stable environment. However, our own experiments had shown (Lübbers et al., 1969) that at high Po2 values even Clark type electrodes with an Ag/AgCl reference electrode and a buffered 0.1 mol KCl solution may drift so much that longer lasting experiments are impossible. Since we wanted to measure the Po2 histogram of the guinea pig brain perfused with a blood-free perfused medium (Heinrich et al., this volume) which was equilibrated with carbogen (95% O2 and 5% CO2), we investigated possibilities to improve the stability of the electrode, particularly at high Po2 values.
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References
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© 1984 Plenum Press, New York
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Yu, B., Baumgärtl, H., Lübbers, D.W. (1984). An Improved Polarographic Multiwire Surface Po2 Electrode, Particularly for Measurement of High Po2 Values. In: Lübbers, D.W., Acker, H., Leniger-Follert, E., Goldstrick, T.K. (eds) Oxygen Transport to Tissue-V. Advances in Experimental Medicine and Biology, vol 169. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1188-1_82
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DOI: https://doi.org/10.1007/978-1-4684-1188-1_82
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