Abstract
Assuming that the majority of readers will be clinical investigators we tried to anticipate what such a specialist may expect to find in this chapter. We concluded that a detailed description of current methods of interferon preparation would not be within the context of this book*. Rather than burden the reader with technical details we decided to give an overview with emphasis on advantages and disadvantages of current production methods and present information about the origin, status of supply, and purity of currently available interferon preparations. Aspects of product quality we assumed would be of foremost importance. Such information we believe will help in the planning of various clinical studies and in the interpretation of results.
An up-to-date and detailed description of production and purification methods is found in Methods in Enzymology, 78, S. Pestka, eds., Academic Press, 1981.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Armstrong, J.A. 1981, Cytopathic effect inhibition assays for interferon: micro-culture plate assay, Methods in Enzymology, 78:381.
Berg, K., and Heron, I. 1981, Antibody affinity chromatography of human leukocyte interferon, Methods in Enzymology, 78:487.
Bodo, G. 1981, Procedures for large-scale production and partial purification of human interferon from lymphocyte (Namalva) cultures, Methods in Enzymology, 78:69.
Cantell, K., Hirvonen, S., Kauppinen, H.-L., and Myllyla, G. 1981a, Production of interferon in human leukocytes from normal donors with the use of Sendai virus, Methods in Enzymology, 78:29.
Cantell, K., Hirvonen, S., and Koistinen, V. 1981b, Partial purification of human leukocyte interferon on a large scale, Methods in Enzymology, 78:499.
de Ley, M., Van Damme, J., Claeys, H., Weening, H., Heine, J.W., Billiau, A., Vermylen, C., and de Somer, P. 1980, Interferon induced in human leukocytes by mitogens: production, partial purification and characterization, Eur. J. Immunol., 10:877.
Epstein, L.B. 1981, Induction and production of immune interferon by mitogen and antigen-stimulated purified lymphocytes cultured in the presence of macrophages, Methods in Enzymology, 78:147.
Familletti, P.C., Costello, L., Rose, CA., and Pestka, S. 1981a, Induction, production, and concentration of interferon produced by a myeloblast culture, Methods in Enzymology, 78:83.
Familletti, P.C., Rubinstein, S., and Pestka, S. 1981b, A convenient and rapid cytopathic effect inhibition assay for interferon, Methods in Enzymology, 78:387.
Fleischmann, W.R., Jr., Georgiades, J.A., Osborne, L.C., and Johnson, H.M. 1979, Potentiation of interferon activity by mixed preparations of fibroblast and immune interferon, Infect. Immun., 26:248.
Friesen, H.-J., Stein, S., and Pestka, S. 1981, Purification of human fibroblast interferon by high-performance liquid chromatography, Methods in Enzymology, 78:430.
Giard, D.J., Loeb, D.H., Thilly, W.G., Wang, D.I.C., and Levine, D.W. 1979, Human interferon production with diploid fibroblast cells grown on microcarriers, Biotechnol. Bioeng., 21:433.
Goeddel, D.V., Kleid, D.G., Bolivar, F., Heynecker, H.L., Yandura, D.G., Crea. R., Hirose, T., Kraszewski, A., Itakura, K., and Riggs, A.D. 1979, Expression in Escherichia coli of chemically synthesized genes for human insulin, Proc. Natl. Acad. Sci. USA, 76:106.
Goeddel, D.V., Yelverton, E., Ullrich, A., Heyneker, H.L., Miozzari, G.F., Holmes, W., Seeburg, P.H., Dull, T., May, L., Stebbing, N., Crea, R., Maeda, S., McCandliss, R., Sloma, A., Tabor, J.M., Gross, M., Familletti, P.C., and Pestka, S. 1980, Human leukocyte interferon produced by E. coli is biologically active, Nature, 287:411.
Gresser, I. 1961, Production of interferon by suspension of human leukocytes, Proc. Soc. Exp. Biol. Med., 108:799.
Havell, E.A., Yip, Y.K., and Vilcek, J. 1978, Characteristics of human lymphoblastoid (Namalva) interferon, J. Gen. Virol., 38:51.
Heine, K..T.W., and Billiau, A. 1981, Purification of human fibroblast interferon by adsorption on controlled-pore glass and zinc-chelate chromatography, Methods in Enzymology, 78:448.
Herschberg, R.D., Gusciora, E.G., Familletti, P.C., Rubinstein, S., Rose, C.A., and Pestka, S. 1981, Induction and production of human interferon with human leukemic cells, Methods in Enzymology, 78:45.
Hobbs, D.S., Moschera, J., Levy, W.P., and Pestka, S. 1981, Purification of interferon produced in a culture of human granulocytes, Methods in Enzymology, 78:472.
Horowitz, B. 1981, Human interferon-properties, clinical application, and production, J. Parenteral Science and Technology, 35:223.
Johnson, H.M., Dianzani, F., and Georgiades, J.A. 1981, Large-scale induction and production of human and mouse immune interferon, Methods in Enzymology, 78:158.
Johnston, M.D., Finter, N.B., and Young, P.A. 1981, Dye uptake method for assay of interferon activity, Methods in Enzymology, 78:394.
Kenny, C., Moschera, J.A., and Stein, S. 1981, Purification of human fibroblast interferon produced in the absence of serum by Cibacron Blue F3GA-agarose and high-performance liquid chromatography, Methods in Enzymology, 78:435.
Klein, G., Dombos, L., and Gothoskar, B. 1972, Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EBV, Int. J. Cancer, 10:44.
Klein, F., and Ricketts, R.T. 1981, Procedures for large-scale production and concentration of lymphoblastoid interferon, Methods in Enzymology, 78:75.
Knight, E. 1981, Purification of human fibroblast interferon prepared in the absence of serum, Methods in Enzymology, 78:417.
Koeffler, H.P., and Golde, D.W. 1978, Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity, Science, 200:1153.
Kwan, S.P., Yelton, D.E., and Scharff, M.D. 1980, Production of monoclonal antibodies, in: “Genetic Engineering: Principles and Methods”, Vol. 2, J.K. Setlow and A. Hollander, eds., Plenum Press, New York.
Langford, M.P., Georgiades, J.A., Stanton, G.J., Danzoni, F., and Johnson, H.M. 1979, Large-scale production and physiochemical characterization of human immune interferon, Infect. Immun., 26:36.
Lin, L.S., and Stewart, W.E., II. 1981, Purification of human leukocyte interferon by two-dimensional Polyacrylamide gel electrophoresis, Methods in Enzymology, 78:481.
Leong, S.S., Horoszewicz, J.S., and Carter, W.A. 1979, Human fibroblast interferon (HFIF): antiproliferative activity of crude and purified preparations, Abstr. Amer. Soc. Microbiol., p.277.
Leong, S.S., and Horoszewicz, J.S. 1981, Production and preparations of human fibroblast interferon for clinical trials, Methods in Enzymology, 78:87.
Miozzari, G.F. 1981, Strategies for obtaining expression of peptide hormones in E. coli in: “Insulins, Growth Hormone, and Recombinant DNA Technology”, J.L. Gueriguian, ed., Raven Press, New York.
Mizrahi, A. 1981, Production of human lymphoblastoid (Namalva) interferon, Methods in Enzymology, 78:54.
Morrissey, J.H. 1981, Silver stain for proteins in Polyacrylamide gels: a modified procedure with enhanced uniform sensitivity, Anal. Biochem., 117:307.
Oakley, B.R., Kirsch, D.R., and Morris, N.R. 1980, A simplified ultrasensitive silver stain for detecting proteins in Polyacrylamide gels, Anal. Biochem., 105:361.
Pritchett, R., Pedersen, M., and Keiff, E. 1976, Complexity of EBV homologous DNA in continuous lymphoblastoid cell lines, Virology, 74:227.
O’Malley, J.A. 1981, Affinity chromatography of human immune interferon, Methods in Enzymology, 78:540.
Rehberg, E., Kelder, B., Hoal, E.G., and Pestka, S. Specific molecular activities of recombinant and hybrid leukocyte interferons, J. Biol. Chem. (in press).
Rubinstein, M., Stein, S., and Udenfriend, S. 1980, Fluorometric methods for analysis of proteins and peptides: principles and applications, Hormonal Proteins and Peptides, 9:1.
Rubinstein, S., Familletti, P.C., and Pestka, S. 1981, Convenient assay for interferons, J. Virol., 37:755.
Rubinstein, M., and Pestka, S. 1981, Purification and characterization of human leukocyte interferons by high-performance liquid chromatography, Methods in Enzymology, 78:464.
Staehelin, T., Hobbs, D.S., Kung, H.-F., Lai, C.-Y, and Pestka, S. 1981, Purification and characterization of recombinant human leukocyte interferon (rIFN-alpha D) with monoclonal antibodies, J. Biol. Chem., 256:9750.
Strander, H., and Cantell, K. 1966, Production of interferon by human leukocytes in vitro, Ann. Med. Exp. Biol. Fenn., 44: 265.
Strander, H., Morgensen, K.E., and Cantell, K. 1975, Production of human lymphoblastoid interferon, J. Clin. Microbiol., 116.
Streuli, M., Hall, A., Boll, W., Stewart, W.E., II, Nagata, S., and Weissmann, C. 1981, Target cell specificity of two species of human interferons-alpha produced in Escherichia coli and of hybrid molecules derived from them, Proc. Natl. Acad. Sci. USA, 78:2848.
Tan, Y.H. 1981a, Induction and production of human interferon with a continuous line of modified fibroblast, Methods in Enzymology, 78:422.
Tan, Y.H. 1981b, Purification of human fibroblast interferon prepared from modified fibroblasts, Methods in Enzymology, 78:422.
Van Damme, J., and Billiau, A. 1981, Large-scale production of human fibroblast interferon, Methods in Enzymology, 78:101.
Von Wussow, P., Chen, Y.-S., Wiranowska-Stewart, M., and Stewart, W.E., II, 1982, Induction of human gamma interferon in lymphoid cells by Staphylococcus enterotoxin B: partial purification, J. Interferon Res., 2:11.
Waldman, A.A., Miller, R.S., Familletti, P.C., Rubinstein, S., and Pestka, S. 1981, Induction and production of interferon with human leukocytes from normal donors with the use of Newcastle disease virus, Methods in Enzymology, 78:39.
Weck, P.K., Apperson, S., Stebbing, N., Gray, P.W., Leung, D., Shepard, H.M., and Goeddel, D.V. 1981, Antiviral activities of hybrids of two major human leukocyte interferons, Nucleic Acid Res., 9:6153.
Wray, W., Boulikas, T., Wray, V.P., and Hancock, R. 1981, Silver staining of proteins in Polyacrylamide gels, Anal. Biochem., 118:197.
Yip, Y.K., Pang, R.H.L., Urban, C., and Vilcek, J. 1981, Partial purification and characterization of human gamma (immune) interferon, Proc. Natl. Acad. Sci. USA, 78:1601.
Zoon, K.C. 1981, Purification and characterization of human interferon from lymphoblastoid (Namalva) cultures, Methods in Enzymology, 78:457.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Plenum Press, New York
About this chapter
Cite this chapter
Ramel, A.H., McGregor, W.C., Dziewanowska, Z.E. (1983). Methods of Preparation. In: Sikora, K. (eds) Interferon and Cancer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1170-6_3
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1170-6_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1172-0
Online ISBN: 978-1-4684-1170-6
eBook Packages: Springer Book Archive