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Modern Fluorescence Spectroscopy pp 143–191Cite as

Principles and Practice of Fluoroimmunoassay Procedures

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Part of the book series: Modern Analytical Chemistry ((MOAC))

Abstract

The immunoassay technique introduced by Yalow and Berson(1, 2) has been one of the most significant advances in analytical methodology in the biological and medical sciences over the past two decades. The power of immunoassay lies in its combination of specificity, versatility, practicability, and potential sensitivity. It can enable the routine determination of specific analytes down to femtomole (10−15 mol) levels in highly complex media such as human blood serum. The practice of academic and diagnostic endocrinology has been revolutionized,(3) and the method is now undergoing major expansion into other areas of clinical science where its advantages are becoming appreciated, such as hematology,(4) cancer detection and treatment,(5) and the monitoring of drug therapy.(6, 7)

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Author notes

  1. D. S. Smith

    Present address: Department of Chemical Pathology, St. Bartholomew’s Hospital, Bartholomew Close, London, EC1A 7HL, UK

Authors and Affiliations

  1. Technia Diagnostics Ltd., City Road, London, EC1V 1JX, UK

    D. S. Smith & Mithal Hassan

  2. Department of Chemical Pathology, St. Bartholomew’s Hospital, Bartholomew Close, London, EC1A 7HL, UK

    R. D. Nargessi

Authors
  1. D. S. Smith
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  2. Mithal Hassan
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  3. R. D. Nargessi
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Editors and Affiliations

  1. Department of Chemistry, University of Tennessee, 37916, Knoxville, Tennessee, USA

    E. L. Wehry

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© 1981 Plenum Press, New York

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Smith, D.S., Hassan, M., Nargessi, R.D. (1981). Principles and Practice of Fluoroimmunoassay Procedures. In: Wehry, E.L. (eds) Modern Fluorescence Spectroscopy. Modern Analytical Chemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1092-1_4

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  • DOI: https://doi.org/10.1007/978-1-4684-1092-1_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1094-5

  • Online ISBN: 978-1-4684-1092-1

  • eBook Packages: Springer Book Archive

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