Summary
Homogeneous immunoglobulin fractions were isolated from sera from several patients affected by subacute sclerosing panencephalitis. In one case, a large amount of plasma was obtained by plasmapheresis. The major immunoglobulin G fractions were purified by ammonium or sodium sulfate precipitation followed by DEAE-ion exchange chromatography and preparative column isoelectric focusing. Measles antibody activity was verified by inhibition of hemagglutination and precipitin reaction with measles or SSPE virus preparations.
Amino acid sequence determinations of the light and heavy chains and of their peptides confirm the homogeneity of the isolated antibody fractions. A comparison of the light chains amino terminal sequences between themselves and with myeloma proteins indicate that SSPE light chains often belong to a variable region subgroup intermediary between the VKII and the VKIII subgroup of chains. An unusually high proportion of SSPE heavy chains belongs to the unblocked VHIII subgroup.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Lowenthal, A.: Agar gel Electrophoresis in Neurology, Elsevier Publishing Co., Amsterdam (1964).
Vandvik, B. and Norrby, E.: Oligoclonal IgG antibody response in the central nervous system to different measles virus antigens in subacute sclerosing panencephalitis. Proc. Natl. Acad. Sci. 70, 1060 (1973).
Vandvik, B., Natvig, J.B. and Norrby, E.: IgGl subclass Restriction of Oligoclonal Measles Virus-Specific IgG Antibodies in Patients with Subacute Sclerosing Panencephalitis and in a Patient with Multiple Sclerosis. Scand. J. Immunol., 6, 651 (1977).
Bollengier, F., Rabinovitch, N. and Lowenthal, A.: Oligoclonal immunoglobulins, light chain rations and free light chains in cerebrospinal fluid and serum from patients affected with various neurological diseases. J. Clin. Chem. Clin. Biochem. 16, 165 (1968).
Strosberg, A.D., Karcher, D. and Lowenthal, A.: Structural homogeneity of h-uman subacute sclerosing panencephalitis antibodies. J. of Immunol. 115, 157 (1975).
Awdeh, Z.L., Williamson, A.R. and Askonas, B.A.: Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature: 219, 66 (1968).
Strosberg, A.D., Jeffery, R.A., Freier, L.J. and Connolly, W.J.: Accelerated liquid isoelectric focusing using a direct current source that maintains constant power. Analyt. Biochem. 69, 76 (1975).
Fleischman, J.B., Porter, R.R. and Press, E.M.: The arrangement of the peptide chains in γ-globulin. Biochem. J., 88, 220 (1963).
Brauer, A.W., Margolies, M.N. and Haber, E.: The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides. Biochemistry. 14, 3029 (1975).
Pisano, J.J. and Bronzert, T.J. Analysis of amino acid phenylthiohydantoins by gas chromatography. J. Biol. Chem., 244, 5597 (1969).
Summers, M.R., Smythers, G.W. and Orozlan, S.: Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets. Analyt. Biochem. 53 624 (1973).
Inglis, A.S., Nicholls, P.W. and Roxburgh, C.M.: Acid hydrolysis of phenylthiohydantoin of amino acids. Aust. J. Biol. Sci. 24, 1247 (1971).
Strosberg, A.D., Marescau, B., Thielemans, K., Vray, B., Karcher, D. and Lowenthal, A.: Cross-idiotypic specificityamong immunoglobulins in subacute sclerosing panencephalitis and multiple sclerosis. Proc. Nato Advanced Study Institute in Neurological Diseases. Antwerp, 1978 (This volume).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1979 Plenum Press, New York
About this chapter
Cite this chapter
Strosberg, A.D., Bollengier, F., Mahler-Rabinovitch, N., Valdimarsson, H., Lowenthal, A. (1979). Structural Studies of Immunoglobulins From Patients Affected by Subacute Sclerosing Panencephalitis. In: Karcher, D., Lowenthal, A., Strosberg, A.D. (eds) Humoral Immunity in Neurological Diseases. NATO ASI Series, vol 24. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1003-7_41
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1003-7_41
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1005-1
Online ISBN: 978-1-4684-1003-7
eBook Packages: Springer Book Archive