Abstract
Under properly defined conditions picornaviruses interrupt host RNA and protein synthesis (1) and subvert the cellular machinery to production of viral protein and RNA. By feeding radiolabeled amino acids to virus-infected cells after cessation of host-protein synthesis, viral protein can be selectively labeled. In a pioneering study, which introduced the now widely used SDS-polyacrylamide gel electrophoresis technique, Summers et al. (2) identified some 14 different virus-specified polypeptides in extracts of poliovirus infected HeLa cells. The net mass of these polypeptides exceeded two-fold or more the known coding capacity of the viral genome. The explanation was later traced to cleavages which took pla.ce during and after synthesis of the viral protein.
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Rueckert, R.R., Matthews, T.J., Kew, O.M., Pallansch, M., McLean, C., Omilianowski, D. (1979). Synthesis and Processing of Picornaviral Polyprotein. In: Pérez-Bercoff, R. (eds) The Molecular Biology of Picornaviruses. NATO Advanced Study Institutes Series, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1000-6_6
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DOI: https://doi.org/10.1007/978-1-4684-1000-6_6
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