Abstract
This review is concerned with the effects of picornavirus infection on the biosynthesis of macromolecules in animal cells. Soon after infection of animal cells with a member of the picornavirus group, there is an inhibition of cellular RNA and protein synthesis. Later in infection, the synthesis of cellular DNA is also decreased. During this time, viral constituents are synthesized and assembly of viral progeny occurs. Late in infection, there is an irreversible loss of the capacity of the cell to synthesize any macromolecules, host or viral, and finally, the cell dies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
MARTIN, E.M. and KERR, I.M. Virus-induced changes in host-cell macromolecular synthesis. In The Molecular Biology of Viruses, Eighteenth Symposium of the Society for General Microbiology, eds., Crawford, L.V. and Stocker, M.G.P. (1968), pp. 15–56. London: Cambridge University Press.
METZ, D.H. Discrimination between viral and cellular macromolecular synthesis. In Control Processes in Virus Multiplication, Twenty-Fifth Symposium of the Society for General Microbiology, eds. Burke, D.C. and Russell W.O. (1975), pp. 323–353. London: Cambridge University Press.
ROIZMAN, B. and SPEAR, P.G. Macromolecular biosynthesis in animal cells infected with cytolytic viruses. Curr. Top. Develop. Biol. (1969), 4, 79–108.
BABLANIAN, R. Structural and functional alterations in cultured cells infected with cytocidal viruses. In Progress in Medical Virology, ed. Melnick, J.L. (1975), Vol. 19 PP. 4O-83. Basel: Karger.
MANAK, M.M. Protein synthesis inhibition by mengovirus. Studies on the role of native 4OS ribosomal subunits. Ph. D. Thesis. (1976), University of Connecticut, pp. 1–154
NAKAI, K. and LUCAS-LENARD, J. Processing of mengovirus precursor polypeptides in the presence of zinc ions and sulfydryl compounds. J. Virol. (1976), 18, 918–925.
PENMAN, S., SCHERRER, K., BECKER, Y. and DARNELL, J.E. Polysomes in normal and poliovirus-infected Hela cells and their relationship to messenger-RNA. Proc. Natl. Acad. Sci. U.S.A. (1963), 49, 654–661.
DALGARNO, L., COX, R.A. and MARTIN, E.M. Polyribosomes in normal Krebs-2 Ascites tumor cells and in cells infected with encephalomyocarditis virus. Biochem. Biophys. Acta. (1967), 138, 316–328.
COLBY, D.S., FINNERTY, Y. and LUCAS-LENARD, J. Fate of mRNA of L-cells infected with mengovirus. J. Virol. (1974), 13, 858–869.
SCHARFF, M.D., SHATKIN, A.J. and LEVINTOW, L. Association of newly formed viral protein with specific polyribosomes. Proc. Natl. Acad. Sci. U.S.A. (1963), 50, 686–694.
PENMAN, S. and SUMMERS, D. Effects on host cell metabolism following synchronous infection with poliovirus. Virology, (1965), 27, 614–620.
HELENTJARIS, T. and EHRENFELD, E. Inhibition of host protein synthesis by UY-inactivated poliovirus. J. Virol. (1977), 21, 259–267.
JACOBSON, M.F. and BALTIMORE, D. Polypeptide cleavages in the formation of poliovirus proteins. Proc. Natl. Acad. Sci. U.S.A. (1968), 61, 77–84.
STEINER-PRYOR, A. and COOPER, P.D. Temperature-sensitive poliovirus mutants defective in repression of host protein synthesis are also defective in structural protein. J, Gen. Virol. (1973), 21, 215–225.
HOLLAND, J. Inhibition of host cell macromolecular synthesis by high multiplicities of poliovirus under conditions preventing virus synthesis. J. Mol. Biol. (1965), 8, 574–581.
COLE, C.N. and BALTIMORE, D. Defective interfering particles of poliovirus. III. Nature of the defect. J. Mol. Biol. (1973), 76, 325–343.
FRANKLIN, R.M. and BALTIMORE, D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harbour Symposium Quantitative Biology. (1962), 21, 175–198.
DARNELL, J.E., GIRARD, M., BALTIMORE, D. and MAIZEL, J.Y. The synthesis and translation of poliovirus RNA. In The Molecular Biology of Viruses, eds. Colter, J.S. and Paranchych, W. (1967), PP: 575–401. New York: Academic Press.
WILLEMS, M. and PENMAN, S. The mechanism of host cell protein synthesis inhibition by poliovirus. Virology, (1966), 30, 355–367.
LEIBOWITZ, R. and PENMAN, S. Regulation of protein synthesis in Hela cells. III. Inhibition during poliovirus infection, J. Virology. (1971), 8, 661–668.
SCHWARTZ, L.B., LAWRENCE, C., THACH, R.E. and ROEDER, E.G. Encephalomyocarditis virus infection of mose plasmacytoma cells. III. Effect on host RNA synthesis and RNA polymerases.J. Virology. (1974), 14, 611–619
APRILETTI, J.W. and PENHOET, E.E. Recovery of DNA-dependent RNA polymerase activities from L-cells after mengovirus infection. Virology, (1974), 61, 597–601.
ENSMINGER, W.D. and TAMM, I. The step in cellular DNA synthesis blocked by Newcastle disease virus or mengovirus infection. Virology, (1970), 40, 152–165.
HAND, R. and OBLIN, C. DNA synthesis in mengovirus-infected cells: Mechanism of inhibition. J. Gen.Virol. (1977), 37, 349–358.
BRAWERMAN, G. Eukaryotic messenger RNA. Ann. Rev. Biochem. (1974), 43, 621–642.
LAWRENCE, G. and THACH, R. Encephalomyocarditis virus infection of mouse plasmocytoma cells. I. Inhibition of cellular protein synthesis. J. Virol. (1974), 14 598–610.
GALLWITZ, D., TRAUB, U. and TRAUB, P. Pate of histone messenger RNA in mengovirus-infected Ehrlich ascites tumor cells. Eur. J. Biochem. (1977), 81 387–393.
SHEINESS, D. and DARNELL, J.E. Polyadenylic acid segment in mRNA becomes shorter with age. Nature New Biol. (1973), 241, 265–268.
KOSCHEL, K. Poliovirus infection and poly(A) sequences of cytoplasmic cellular RNA. J. Virol. (1974), 13, 1O61–1O66.
SHATKIN, A.J. Capping of eukaryotic mRNAs. Cell (1976), 9, 645–653.
PERNANDEZ-MDNOZ, R. and DARNELL, J.E. Structural difference between the 5’ termini of viral and cellular mRNA in poliovirus-infected cells: Possible basis for the inhibition of host protein synthesis. J. Virol. (1976), 18, 719–726.
DOYLE, S. and HOLLAND, J. Virus-induced interference in heterologously infected HeLa cells. J. Virol. (1972), 9, 22–28.
MUDD, J.A. and SUMMERS, D.P. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology, (1970), 18, 328–340.
WERTZ, G.W. and YOUNGNER, J.S. Inhibition of protein synthesis in L-cells infected with YSY. J. Virol. (1972), 9, 85–89.
MOYER, S.A., GRUBMM, J.J., EHEENEELD, E. and BANERJEE, A.K. Studies on the in vivo messenger RHA species of vesicular stomatitis virus. Virology, (1975), 67, 465–473.
EHREEPELD, E. and LUID, H. Untranslated vesicular stomatitis virus messenger RNA after poliovirus infection. Virology, (1977), 80, 297–308.
WENGLER, G. and WENGLER, G. Medium hypertonicity and polyribosome structure in HeLa cells. The influence of hjrpertonicity of the growth medium on polyribosomes in HeLa cells. Eur. J. Biochem. (1972), 27, 162–173.
SABORIO, J.L., PONG, S.S. and KOCH, G. Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. J. Mol. Biol. (1974), 85, 195–211.
NUSS, D.L., OPPERMAN, H. and KOCH, G. Selective blockage of initiation of host protein synthesis in RNA virus-infected cells. Proc. Natl. Acad. Sci. U.S.A. (1975), 72, 1258–1262.
NUSS, D.L., and KOCH, G. Translation of individual host mRNAs in MPC-11 cells is differentially suppressed after infection by vesicular stomatitis virus. J. Virol. (1976), 19, 272–278.
CARRASCO, L. The inhibition of cell functions after viral infection: A proposed general mechanism. PEBS Letters (1977), 76, 11–15.
CARRASCO, L. and SMITH, A.E. Sodiumions and the shut-off of host cell protein synthesis by picornaviruses. Nature (London), (1976), 264, 8O7–8O9.
EGBERTS, E., HACKETT, P.B. and TRAUB, P. Alteration of the intracellular energetic and ionic conditions by mengovirus infection of Ehrlich ascites tumor cells and its influence on protein synthesis in the midphase of infection. J. Virol. (1977), 22, 591–597.
ABREU, S. and LUCAS-LENARD, J. Cellular protein synthesis shut-off by mengovirus: Translation of nonviral and viral mRNAs in extracts from uninfected and infected Ehrlich ascites tumor cells. J. Virol. (1976), 18, 184–192.
LODISH, H.P. Model for the regulation of mRNA translation applied to haemoglobin synthesis. Nature (London), (1974), 251, 385–388.
GOLINI, P., THACH, S., LAWRENCE, C. and THACH, R. Regulation of protein synthesis in EMC virus-infected cells. In Animal Virology, IV. ICN-UCLA Symposia on Molecular and Cellular Biology, eds., Baltimore, D., Huang, A.S. and Pox, C.P. (1976), pp. 717–754. New York: Academic Press.
GOLINI, P., THACH, S.S., BIRGE, C.H., SAPER, B., MERRICK, W.C. and THACH, R.E. Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor. Proc. Natl. Acad. Sci. U.S.A. (1976), 73, 3040–3044.
COOPER, P.D., STEINER-PRYOR, A. and WRIGHT, P.J. A proposed regulator for poliovirus: The equestron. Intervirology, (1975), 1, 1–10.
WRIGHT, P.J. and COOPER, P.D. Poliovirus proteins associated with ribosomal structures in infected cells. Virology, (1974), 59, 1–20.
MEDYEDKINA, O.A., SCARLAT, I.Y., KALININA, N.O. and AGOL, V.I. Virus-specific proteins associated with ribosomes of Krebs-II cells infected with encephalomyocarditis virus. PEBS Letters (1974), 39, 4–8.
MANAK, M., ABREU, S. and LUCAS-LENARD, J. Association of mengovirus proteins with host cell native 4OS ribosomal subunits. INSERM (1975), 47, 387–396.
SVITKIN, Y.Y., UGAROYA, T.Y., GINEYSKAYA, V.A., KALININA, N.O., SCARLAT, I.Y. and AGOL, V.I. Efficiency of translation of viral and cellular mRNAs in extracts from cells infected with encephalomyocarditis virus. Intervirology, (1974), 4, 214–220.
KAUPMANN, Y., GOLDSTEIN, E. and PENMAN, S. Poliovirus-induced inhibition of polypeptide initiation in vitro on native polyribosomes. Proc. Natl. Acad. Sci. U.S.A. (1976), 73, 1834–1838.
HELENTJARIS, T. and EHRENFELD, E. Control of protein synthesis in extracts from poliovirus-infected cells. I. mRNA discrimination by crude initiation factors. J. Virol. (1978), 26, 510–521.
ROSE, J.K., TRACHSEL, H., LEONG, K. and BALTIMORE, D. Inhibition of translation by poliovirus: Inactivation of a specific initiation factor. Proc. Natl. Acad. Sci. U.S.A. (1978), 75, 2732–2736.
SHAPRITZ, D.A., WEINSTEIN, J.A., SAFER, B., MERRICK, W.C., WEBER, L.A., HICKEY, E.D. and BAGLIONI, E. Evidence for role of m7G5’ — phosphate group in recognition of eukaryotic mRNA by initiation factor IP-M3. Nature (London), (1976), 261, 291–294.
HACKETT, P.B., EGBERTS, E. and TRAUB, P. Translation of ascites and mengovirus RNA in fractionated cell-free systems from uninfected and mengovirus-infected Ehrlich-ascites tumor cells, Eur. J. Biochem. (1978), 83, 341–352.
HACKETT, P.B., EGBERTS, E. and TRAUB, P. Selective translation of mengovirus RNA over host mRNA in homologous, fractionated, cell-free translational systems from Ehrlich-ascites tumor cells. Eur. J. Biochem. (1978), 83, 353–361.
HUNT, T. and EHRENFELD, E. Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis. Nature New Biology (1971), 230, 91–94.
EHRENFELD, E. and HUNT, T. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates. Proc. Natl. Acad. Sci. U.S.A. (1971), 68 1075–1078.
CELMA, M.L. and EHRENFELD, E. Effect of poliovirus double-stranded RNA on viral and host cell protein synthesis. Proc. Natl. Acad. Sci. U.S.A. (1974), 71, 2440–2444.
COLLINS, P. and ROBERTS, W. Mechanism of mengovirus-induced cell injury in L-cells: Use of inhibitors of protein synthesis to dissociate virus-specific events. J. Virol. (1972), 10, 969–978.
LEVIN, D. and LONDON, I.M. Regulation of protein synthesis: Activation by double-stranded RNA of a protein kinase that phosphorylates eukaryotic initiation factor 2. Proc. Natl. Acad. Sci. U.S.A. (1978), 75, 1121–1125.
CORDELL-STEWART, B. and TAYLOR, M.W. Effect of viral double-stranded RNA on mammalian cells in culture: Cytotoxicity under conditions preventing viral replication and protein synthesis. J. Virol. (1973), 12, 36O-366.
CORDELL-STEWART, B. and TAYLOR, M.W. Effect of viral double-stranded RNA on protein synthesis in intact cells. J. Virol. (1975), 11, 252–237
NISHIOKA, Y. and SILYERSTEIN, S. Alterations in the protein synthetic apparatus of Friend erythroleukemia cells infected with vesicular stomatitis virus or Herpes simplex virus. J. Virol. (1978), 25, 422–426.
YAUGHAN, M.H., PAWLOWSKI, P.J. and FORCHHAMMER, J. Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids. Proc. Natl. Acad. Sci. U.S.A. (1971), 68, 2057–2061.
VAN VENROOIJ, W.J.W., HBNSHAW, E.G. and HIESCH, G.A. Effects of deprival of glucose or individual amino acids on polyribosome distribution and rate of protein synthesis in cultured mammalian cells. Biochem. Biophys. Acta. (1972), 259, 127–137.
GEUMMT, P. and GEUMMT, I. Studies on the role of uncharged tENA in pleiotypic response of animal cells. Eur. J. Biochem. (1976), 64. 307–312.
WAEEINGTON, E.G., WEATTEN, N. and HEGHTMAN, E. L-histidinol inhibits specifically and reversibly protein and ribosomal EHA synthesis in mouse L-cells. J. Biol. Chem. (1977), 252, 5251–5257.
LEYIKE, E.M., BEGKEE, Y., BOOHE, G.W. and EAGLE, H. Contact inhibition, macromolecular synthesis, and polyribosomes in cultured human diploid fibroblasts. Proc. Natl. Acad. Sci. U.S.A. (1965), 53, 350–356
GILOH, H. and MAGER, J. Inhibition of peptide chain initiation in lysates from ATP-depleted cells. I. Stages in the evolution of the lesion and its reversal by thiol compounds, cyclic AMP or purine derivatives and phosphorylated sugars. Biochem. Biophys. Acta. (1975), 414, 293–308.
McGOEMIGK, W. and PENMAN, S. Regulation of protein synthesis in HeLa cells: Translation at elevated temperatures. J. Mol. Biol. (1969), 39, 315–333.
FAN, H. and PENMAN, S. Eegulation of protein synthesis in mammalian cells. Inhibition of protein synthesis at the level of initiation during mitosis. J. Mol. Biol. (1970), 50, 655–670.
HERSHKO, A., MAMONT, P., SHIELDS, E. and TOMKINS, G.M. Pleiotypic response. Nature New Biology (197l), 232, 206–211.
MAECUS, P.I. and ROBINS, E. Viral inhibition in the metaphase-arrest cell. Proc. Natl. Acad. Sci. U.S.A. (1965), 50, 1156–1164.
SEIRAM, G. and TAYLOR, M.W. Purineless death: Eibosomal ENA turnover in a purine-starved ade- mutant of Ghinese hamster cells. J. Biol. Chem. (1977), 252, 5350–5355.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1979 Plenum Press, New York
About this chapter
Cite this chapter
Lucas-Lenard, J.M. (1979). Inhibition of Cellular Protein Synthesis After Virus Infection. In: Pérez-Bercoff, R. (eds) The Molecular Biology of Picornaviruses. NATO Advanced Study Institutes Series, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1000-6_4
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1000-6_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1002-0
Online ISBN: 978-1-4684-1000-6
eBook Packages: Springer Book Archive