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In Vitro Translation of Picornavirus RNA

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The Molecular Biology of Picornaviruses

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 23))

Abstract

Although studies of picornavirus proteins and their biosynthesis in vivo have been conducted with approximately equal intensity for both poliovirus and encephalomyocarditis (EMC) virus (l), the majority of in vitro translation studies have been performed using the mouse virus RNA. Beginning in the early 1970’s, several different laboratories reported the synthesis of virus-specific proteins in preincubated cell-free systems derived from Krebs II, Ehrlich or mouse plasmacytoma ascites cells, or from cultured L cells (2–13). In general, a variety of different sized polypeptides, including those with molecular weights over 100,000 daltons, were produced, but, unlike the various viral proteins synthesized in vivo, the in vitro products appear to arise from premature termination of translation rather than from proteolytic cleavage of a complete translate. Complete translation of the entire viral RNA molecule may occur in rare instances (5), but most of the polypeptides synthesized in vitro contain amino acid sequences derived from a common amino terminus, and the majority of the products contain the tryptic (or cyanogen bromide) peptide fragments which are present in the virus capsid proteins. These proteins are known to be encoded by nucleotide sequences within the 5′ half of the viral RNA (l). A diagram of the overlap in amino sequences of the major translation products (I-VI) of EMC virus RNA by a Krebs or Ehrlich ascites cell-free system is shown below. The data are from Hunt (13).

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, The University of Utah, 84132, Salt Lake City, Utah, USA

    Elvera Ehrenfeld

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  1. Elvera Ehrenfeld
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Editors and Affiliations

  1. Laboratory of Virology and Molecular Biology, Institute of Anatomy, University of Zurich, Zurich, Switzerland

    R. Pérez-Bercoff

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© 1979 Plenum Press, New York

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Ehrenfeld, E. (1979). In Vitro Translation of Picornavirus RNA. In: Pérez-Bercoff, R. (eds) The Molecular Biology of Picornaviruses. NATO Advanced Study Institutes Series, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1000-6_11

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  • DOI: https://doi.org/10.1007/978-1-4684-1000-6_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1002-0

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