Choice of Cell Substrate for Biological Products
The methods at present adopted for the control of the substrate for production of modified live virus vaccines are well established. They involve maintaining colonies of chickens, ducks or rabbits which are the source of primary cells for making such vaccines. These colonies are regularly monitored to exclude infections. The use of human diploid cell lines like WI38 and MRC5 are now internationally accepted as satisfactory substrates for these vaccines and bring the benefits of a defined seed system to the cell substrates as well as to the virus seed. These benefits are consistency and stability; and the ability to use a pretested seed.
KeywordsSendai Virus Cell Substrate Oral Poliovirus Vaccine Monkey Kidney Cell Hamster Cheek Pouch
Unable to display preview. Download preview PDF.
- 4.Pirquet C.F., and Schick B. Serum Sickness. Translated by Bela Schick. Wiliams Wilkins, Baltimore, 1951.Google Scholar
- 5.Hull R.N., Minner J.R. and Mascoli G.C New agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey kidney tissues and directly from tissues and excreta. American Journal of Hygiene 68: 31–44, 1959.Google Scholar
- 6.Stones P.B. Production and control of live oral poliovirus vaccine in WI-38 human diploid cells. Joint WHO/IABS Symposium on the standardization of cell substrates for the production of virus vaccines, Geneva, Dec. 1976. Develop. biol. Standard., 37: 251–253. S. Karger, Basel 1977.Google Scholar
- 7.Christofinis G.J. and Finter N.B. The preparation of Interferon from lymphoblastoid cell lines. Proceedings of Symposium. Preparation, Standardization and clinical use of Interferon. Zagreb, June 8–9, 1977.Google Scholar
- 10.Fantes K.H. Workshop on Clinical Trials with Interferon. National Institute of Allergy and Infectious Diseases and National Cancer Institute, Bethesda. March 21–23, 1978.Google Scholar
- 12.Beale, A.J. D antigen content in poliovaccine as a measure of potency. Lancet 2:1166, 1961.Google Scholar
- 13.Salk J., Cohen H., Fillastre, et al. Killed poliovirus antigen titration in humans. Developments in Biological Standardization: Standardization and use of vaccines in the developing countries. Proceedings 15th International congress, April 1978. International Association of Biological Standardization (in press).Google Scholar
- 14.van Wezel A.L., van Steenis G., Hannick Ch.A. and Cohen H. Developments in Biological Standardization: Standardization and use of vaccines in the developing countries. Proceedings 15th International Congress, April 1978. International Association of Biological Standardization (in press).Google Scholar
- 17.Haase A.T., Mautner V. and Pereira H.G. The immugenicity of adenovirus type 5 Structural proteins. Journal Immunology, 108: 483–485, 1972.Google Scholar