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Quantitation in the Evaluation of Cell Substrates for Viral Vaccine Production

  • Wade P. Parks
  • Elizabeth S. Hubbell
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 118)

Abstract

Although the subject of cell substrates for viral production has recently been reviewed (1) certain points of considerable interest and relevance were not discussed. Issues of benefits and risks have been discussed in the present symposium in the preceding paper (2). In the present paper, we would like to stress the need for quantitative data related to genetic and presumably nongenetic alterations in various cell substrates. These changes may occur in the cells themselves or in their products. First, in order to discuss the issues of cell substrate selection certain definitions are necessary.

Keywords

Mouse Mammary Tumor Virus Cell Substrate Mammary Tumor Cell Line Rubella Virus Vaccine Mouse Mammary Tumor Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Proceedings, Joint WHO/IABS. Symposium on the Standardization of Cell substrates for the Production of Virus Vaccines. S. Karger A.G. Basel. 1977.Google Scholar
  2. 2.
    Petricciani, J.C. Cell substrates in biologics production: Factors affecting acceptability. Preceding paper in symposium. 1978.Google Scholar
  3. 3.
    Klein, G., Pearson, G., Nadkarni, J.S., et al. Relation between Epstein-Barr viral and cell membrane immunofluorescence on Burkitt tumor cells. I. Dependence of cell membrane immunofluorescence on the presence of EB virus. J. Exp. Med. 128:1011–1020, 1968.PubMedCrossRefGoogle Scholar
  4. 4.
    Benveniste, R.E., Lieber, M.M., Livingston, D.M. et al. Infectious type-C virus isolated from a baboon placenta. Nature 248:17–20, 1974.PubMedCrossRefGoogle Scholar
  5. 6.
    Gallo, R.C. RNA Viruses, Genes, and Cancer in Genetics of Human Cancer, Edited by J.J. Mulvihill, R.W. Miller, J.F. Fraumeni, Jr. New York, Raven Press. 1977.Google Scholar
  6. 7.
    Huebner, R.J., Todaro, G.J. Oncogenes of RNA tumor viruses as determinants of cancer. Proc. Natl. Acad. Sci. 64:1087–1094, 1969.PubMedCrossRefGoogle Scholar
  7. 8.
    Todaro, G.J, Sherr, C. J., Sen A. Endogenous new world primate type-C viruses isolated from an owl monkey (Aotus trivirgatus). Proc. Natl. Acad. Sci. 75:1004–1008, 1978.PubMedCrossRefGoogle Scholar
  8. 9.
    Todaro, G.J., Benveniste, R.E., Sherwin, S.A. et al. MAC-1, a newly genetically transmitted type-C virus of primates: “low frequency” activation from stumptail monkey cell cultures. Cell 13:775–782, 1978.PubMedCrossRefGoogle Scholar
  9. 10.
    Benveniste, R.E., Todaro, G.J. Evolution of type-C viral genes. I. Nucleic acid from baboon, type-C virus as a measure of divergence among primate species. Proc. Natl. Acad. Sci. 71:4513–4518, 1974.Google Scholar
  10. 11.
    Benveniste, R.E., Callahan, R., Sherr, C.J. et al. Two distinct endogenous type-C viruses isolated from the Asian rodent Mus cervicolor: conservation of virogene sequences in related rodent species. J. Virol. 21:849–862, 1977.PubMedGoogle Scholar
  11. 12.
    Todaro, G.J., Sherr, C.J., Benveniste, R.E. Baboons and their close relatives are unusual among primates in their ability to release nondefective endogenous type-C viruses. Virology 72: 278–262, 1976.PubMedCrossRefGoogle Scholar
  12. 13.
    Stephenson, J.R., Aaronson, S.A. Endogenous type-C viral expression in primates. Nature 266:469–472, 1977.PubMedCrossRefGoogle Scholar
  13. 14.
    Smotkin, D., Gianni, A.M., Rozenblatt, S. et al. Infectious viral DNA of murine leukemia virus. Proc. Natl. Acad. Sci. 72: 4910–4913, 1975.PubMedCrossRefGoogle Scholar
  14. 15.
    Wang, C.H., Duesberg, P., Beemon, U., et al. Mapping RN’ase T1 — resistant oligonucleotides of avian tumor virus RNA’s: sacoma-specific olignucleotides are near the poly(A) end and olignoucleotides common to sarcoma and non-transforming viruses are at the poly(A) end. J. Virol. 16:1051–1070.Google Scholar
  15. 16.
    Hartley, J.W., Wolford, N., Old, L.J., et al. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc. Natl. Acad. Sci: 74:789–792, 1977.PubMedCrossRefGoogle Scholar
  16. 17.
    Troxler, D.H., Boyers, J.U., Parks, W.P., et al. Friend strain of spleen focus-forming virus: a recombinant between mouse type-C ecotropic viral sequences and sequences related to xenotropic virus. J. Virol. 22:361–372, 1977.PubMedGoogle Scholar
  17. 18.
    Aaronson, S.A., Stephenson, J.R. Endogenous type-C RNA viruses of mammalian cells. Biochem. Biophys. Acta. 458:323–354, 1976.PubMedGoogle Scholar
  18. 19.
    Rapp, U.R., Todaro, G.J. Generation of oncogenic type-C viruses. Rapid leukemia viruses from C3H mouse cells. Science, In press, 1978.Google Scholar
  19. 20.
    Parks, W.P., Hubbell, E.S., Goldberg, R.J., et al: High frequency variation in mammary tumor expression in cell culture. Cell 8:87–93, 1976.PubMedCrossRefGoogle Scholar
  20. 21.
    Coffino, P., Scharff, M. Rate of somatic mutation in immunoglobulin production of mouse myeloma cells. Proc. Natl. Acad. Sci. 68:219–223, 1971.PubMedCrossRefGoogle Scholar
  21. 22.
    Young, H.A., Shih, T., Scolnick, E.M. et al. Steroid induction of mouse mammary tumor virus. Effect upon synthesis and degradation of viral RNA. J. Virol. 21:139–146, 1977.Google Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Wade P. Parks
    • 1
  • Elizabeth S. Hubbell
    • 1
  1. 1.Department of MicrobiologyUniversity of Miami School of Medicine and Veterans Administration HospitalMiamiUSA

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