Abstract
The method used for bioassay of insecticidal activity is a vital cog in the wheel of structure-activity studies. An assay preparation which consists of recording muscle potentials from the dorsolongitudinal flight muscles of house fly gives sophisticated information on neural coordination in the thoracic ganglion with a relatively simple recording procedure. Quantitative responses are measured as the time from treatment until a disruption appears in neural coordination. The type of disruption in neural coordination gives a qualitative measure of poisoning. By applying compounds directly on the exposed thoracic ganglion, or the exposed and desheathed ganglion, some measure of intrinsic activity may be obtained. Using this approach, evidence for the mechanism of resistance to insecticides has been obtained for house fly strains resistant to organophosphates or pyrethroids, and intrinsic activity has been obtained for carbamates, organophosphates, pyrethroids and other chemicals with insecticidal activity.
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© 1979 Plenum Press, New York
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Miller, T.A. (1979). Mode of Action of Insecticides: Insights Gained from Neurophysiological Preparations of Intact or Dissected Insects. In: Narahashi, T. (eds) Neurotoxicology of Insecticides and Pheromones. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0970-3_5
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DOI: https://doi.org/10.1007/978-1-4684-0970-3_5
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