Abstract
The purpose of this study is to illustrate some data on the use of P-lipase C to elucidate the membrane structure of intact mitochondria and submitochondrial particles (1,2). Fig. 1 shows the effect of P-lipase C from B. cereus on the fluorescence of mitochondrial NADH (3). The fluorescence is maximal when the mitochondria are in a tightly coupled state and decreases parallel to uncoupling. The extent of uncoupling increases proportionally to the amount of P-lipase C and occurs always after a lag phase. In the same Figure it is also shown the rapid abolition of the fluorescence caused by the powerful uncoupler FCCP.
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© 1976 Plenum Press, New York
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Azzone, G.F., Viola, E., Strinna, E., Bragadin, M. (1976). Effect of P-Lipase C on the Structure and Function of Mitochondria and Sonic Fragments. In: Porcellati, G., Amaducci, L., Galli, C. (eds) Function and Metabolism of Phospholipids in the Central and Peripheral Nervous Systems. Advances in Experimental Medicine and Biology, vol 72. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0955-0_2
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DOI: https://doi.org/10.1007/978-1-4684-0955-0_2
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