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Effect of Concanavalin A and Phytohemagglutinin on the Modification of Immunogenicity of Canine Kidney Allografts

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Concanavalin A

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 55))

Abstract

Mongrel dog kidneys were allografted to unrelated nephrectomized recipients which were then treated with subimmunosuppressive doses of azathioprine (2.5 mg/kg/day). Dog kidneys treated in vitro with perfusates containing concanavalin A (Con A) or phytohemagglutinin-P (PHA) survived as long as 60 days (mean 39.8 ± 4.3) after transplantation, whereas normal kidneys survived less than 16 days. The optimal prolongation was achieved by perfusing the kidneys with 500 ml Ringer’s lactate containing 25 mg/L Con A, 25 4°C. Lesser effects were achieved with higher or lower concentrations of Con A, or with perfusions carried out at 25°C. Most evidence suggests that Con A and PHA bind to cell surfaces and interfere with the perception of the graft antigens by the host.

Supported by United States Public Health Service Grants #AM 13083 and #CA 11605 and Grant #DRG 1186 from the Damon Runyon Memorial Fund for Cancer Research.

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Authors and Affiliations

  1. University of Minnesota, Minneapolis, Minnesota, USA

    Luis H. Toledo-Pereyra, Clive O. Callender, Prasanta K. Ray, John S. Najarian & Richard L. Simmons

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  1. Luis H. Toledo-Pereyra
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  2. Clive O. Callender
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  3. Prasanta K. Ray
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  4. John S. Najarian
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  5. Richard L. Simmons
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Editors and Affiliations

  1. Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Tushar K. Chowdhury  & A. Kurt Weiss  & 

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© 1975 Plenum Press, New York

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Toledo-Pereyra, L.H., Callender, C.O., Ray, P.K., Najarian, J.S., Simmons, R.L. (1975). Effect of Concanavalin A and Phytohemagglutinin on the Modification of Immunogenicity of Canine Kidney Allografts. In: Chowdhury, T.K., Weiss, A.K. (eds) Concanavalin A. Advances in Experimental Medicine and Biology, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0949-9_17

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  • DOI: https://doi.org/10.1007/978-1-4684-0949-9_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-0951-2

  • Online ISBN: 978-1-4684-0949-9

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