Abstract
DNA replication is carefully regulated in bacteria which results in a constant average number of genomes per cell in an exponentially growing population. This applies to the bacterial chromosome as well as to plasmids carried by the cells. Despite extensive studies on DNA synthesis there is very little knowledge available so far about the mechanisms involved in the regulation of DNA replication. It is generally believed that the control is exerted at the level of initiation (Pritchard, 1974). Mutant plasmids which show an increased number of copies compared to the wild type plasmid, so called copy mutants, have been isolated from several plasmids (Nordström et al., 1972;Tmmis and Winkler, 1973; Kool and Nijkamp, 1974; Morris et al., 1974; Matsubara and Takeda, 1975). Such plasmid mutants may be used as a tool in studies of control of DNA replication. In this paper we will report on studies with copy number control mutants of the plasmid R1 in Escherichia coli K-12.
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© 1978 Plenum Press, New York
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Uhlin, B.E., Gustafsson, P., Molin, S., Nordström, K. (1978). Copy Number Control Mutants of the R Plasmid R1 in Escherichia Coli . In: Molineux, I., Kohiyama, M. (eds) DNA Synthesis. NATO Advanced Study Institutes Series, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0844-7_55
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DOI: https://doi.org/10.1007/978-1-4684-0844-7_55
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