Abstract
We have a more detailed understanding of the mechanism of replication of the DNA of bacteriophage ØX174 than of any other organism, largely because of its small size and the concentrated efforts of a number of laboratories. The viral DNA is a circular, single-stranded DNA molecule of 5,375 nucleotides, and the sequence of these is known (Sanger et al., 1977). After infection of E.coli, the first event is the synthesis of a complementary strand to form the duplex parental replicative form (RF). This RF is transcribed and then replicated several times to form progeny RF molecules. Finally, synthesis of viral single-stranded DNA occurs and progeny virus particles are produced. A more extensive discussion of these and other events in the virus life cycle can be found in reviews by Denhardt (1975,1977).
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Denhardt, D.T., Hours, C. (1978). The Present Status of ØX174 DNA Replication in Vivo. In: Molineux, I., Kohiyama, M. (eds) DNA Synthesis. NATO Advanced Study Institutes Series, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0844-7_50
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DOI: https://doi.org/10.1007/978-1-4684-0844-7_50
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