Abstract
The identification of three classes of DNA polymerases (α, β and γ) in mammalian cells (1) has naturally led to the question of the role of these DNA polymerases in DNA replication and repair. This paper will present studies on rat neurons DNA polymerases which are pertinent to this question. Forebrain neurons were selected for several reasons:
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(a)
they are essentially unable to replicate their DNA, undergo mitosis and proliferate after birth (2-4).
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(b)
they can be isolated from glial cells (5,6) and represent an in vivo intact unicellular system which can be used to correlate development with the capacity to perform DNA replication and DNA repair synthesis.
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(c)
synaptic end knobs contain mitochondria completely unassociated with nuclei and these structures can be isolated as distinct particles (synaptosomes) surrounded by a closed membrane (7), thus allowing the isolation of mitochondrial DNA polymerase and in vitro studies on the role of mt-polymerase in the replication and repair of mitochondrial DNA.
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© 1978 Plenum Press, New York
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Hübscher, U., Kuenzle, C.C., Spadari, S. (1978). Neurons as a Model System for In Vivo Studies of the Possible Function of DNA Polymerases in DNA Replication and Repair Synthesis During Development. In: Molineux, I., Kohiyama, M. (eds) DNA Synthesis. NATO Advanced Study Institutes Series, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0844-7_43
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DOI: https://doi.org/10.1007/978-1-4684-0844-7_43
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