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Recombinational Mapping of the polA Locus of Escherichia Coli K12: Genetic Fine Structure

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DNA Synthesis

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 17))

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Abstract

Recently we described a series of experiments by which the orientation of the E.coli polA cistron was established with respect to the linked genetic loci metE and rha (Kelley and Grindley, 1967A). It was possible to orient the cistron unambiguously because of the unique enzymic properties of the wild type polA gene product, DNA polymerase I, and the properties of the products of three allelic polA mutations, polA1,polA6 and polA107.

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References

  • Bachmann, B.J., Low, K.B. and Taylor, A.L. (1976) Bact. Revs. 40: 116–167

    CAS  Google Scholar 

  • Chase, J.W. and Masker, W.E. (1977) J. Bact. 130:667–675 Cooper, P. (1977) Molec. Gen. Genet. 150: 1–12

    Google Scholar 

  • Demerec, M. (1963) Genetics 48: 1519–1531

    PubMed  CAS  Google Scholar 

  • Grindley, N.D.F. and Kelley, W.S. (1976) Molec. Gen. Genet. 143: 311–318

    Google Scholar 

  • Jacobsen, H., Klenow, H. and Overgaard-Hansen, K. (1974) Eur. J. Biochem. 45: 623–627

    Article  PubMed  CAS  Google Scholar 

  • Kato, T. and Kondo, S. (1970) J. Bact. 104: 871–881

    PubMed  CAS  Google Scholar 

  • Kelley, W.S. and Grindley, N.D.F. (1976a) Molec. Gen. Genet. 147: 307–314

    Google Scholar 

  • Kelley, W.S. and Grindley, N.D.F. (1976b) Nucleic Acid Research 3: 2971–2984

    Google Scholar 

  • Kingsbury, D.T. and Helinski, D.R. (1973b) Genetics 74: 17–31

    PubMed  CAS  Google Scholar 

  • Klenow, H. and Henningsen, I. (1970) Proc. Nat. Acad. Sci. ( Wash. ) 65: 168–175

    Google Scholar 

  • Konrad, E.B. (1977) J. Bact. 130: 167–172

    PubMed  CAS  Google Scholar 

  • Konrad, E.B. and Lehman, I.R. (1974) Proc. Nat. Acad. Sci. ( Wash. ) 71: 2048–2051

    Google Scholar 

  • Lehman, I.R., Uyemura, E.G.: DNA Polymerase I: Science 193: 963–969 (1976).

    PubMed  CAS  Google Scholar 

  • Olivera, B.M. and Bonhoeffer, F. (1974) Nature (Lond.) 250:513–514 Rosner, J.L. (1972) Virology 48: 679–689

    Google Scholar 

  • Uyemura, D., Eichler, D.C. and Lehman, I.R. (1976) J. Biol. Chem. 251: 4085–4089

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biological Sciences Mellon Institute of Science, Carnegie-Mellon University, 4400 Fifth Avenue, Pittsburgh, Pa., 15213, USA

    W. S. Kelley & J. A. Reehl

Authors
  1. W. S. Kelley
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  2. J. A. Reehl
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  3. N. C. Ledonne Jr.
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Editor information

Editors and Affiliations

  1. Imperial Cancer Research Fund, London, England

    Ian Molineux

  2. University of Paris, Paris, France

    Masamichi Kohiyama

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© 1978 Plenum Press, New York

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Kelley, W.S., Reehl, J.A., Ledonne, N.C. (1978). Recombinational Mapping of the polA Locus of Escherichia Coli K12: Genetic Fine Structure. In: Molineux, I., Kohiyama, M. (eds) DNA Synthesis. NATO Advanced Study Institutes Series, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0844-7_35

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  • DOI: https://doi.org/10.1007/978-1-4684-0844-7_35

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-0846-1

  • Online ISBN: 978-1-4684-0844-7

  • eBook Packages: Springer Book Archive

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