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DNA Synthesis pp 267–279Cite as

In Vivo Synthesis and Properties of Uracil-Containing DNA

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Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 17))

Abstract

All known living cells contain thymine in their DNA, but it is not clear why uracil, which base pairs adenine as does thymine, would not suffice. A considerable expenditure of energy is required to synthesize dTTP from dUTP, and the pathway shown in Fig. 1 suggests that dUTP is an obligate precursor of dTTP in wild type Escherichia coli, and probably most other organisms. An alternate route could be the salvage of thymine or thymidine, but it is not known how much dTTP is synthesized by this pathway in various organisms. In wild type E.coli probably very little, if any, dTTP is synthesized in this manner (1).

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References

  1. Kammen, H.O. (1967) Biochim. Biophys. Acta 134: 301–311

    CAS  Google Scholar 

  2. Bessman, M.J., I.R. Lehman, J. Adler, S.B. Zimmerman, E.S. Simms and A. Kornberg (1958) Proc. Natl. Acad. Sci. USA

    Google Scholar 

  3. Bertani, L.E., A. Haggmark and P. Reichard (1963) J. Biol. Chem. 238: 3407–3413

    PubMed  CAS  Google Scholar 

  4. Greenberg, G.R. and R.L. Somerville (1963) Proc. Nat. Acad. Sci. USA 48: 247–257

    Article  Google Scholar 

  5. Tye, B., P. Nyman, I.R. Lehman, S. Hochhauser and B. Weiss (1977) Proc. Nat. Acad. Sci. USA 74: 154–157

    Article  PubMed  CAS  Google Scholar 

  6. Lindahl, T. (1974) Proc. Nat. Acad. Sci. USA 71: 3649–3653

    CAS  Google Scholar 

  7. Lindahl, T. and B. Nyberg (1974) Biochemistry 13: 3405–3410

    Article  PubMed  CAS  Google Scholar 

  8. Lindahl, T. (1976) Nature 259: 64–66

    Article  PubMed  CAS  Google Scholar 

  9. Weiss, B. and C. Milcarek (1974) In: Methods in Enzymology (eds. L. Grossman and K. Moldave) Vol XXIX, Academic Press, Inc. New York., p180–193

    Google Scholar 

  10. Olivera, B.M., B. Tye and I.R. Lehman (1977) Federation Proceed. 36: 2020

    Google Scholar 

  11. Konrad, E.B. and I.R. Lehman (1975) Proc. Nat. Acad. Sci. USA 72: 2150–2154

    Google Scholar 

  12. Hochhauser, S.J. and B. Weiss (1976) Federation Proceed. 35: 1492

    Google Scholar 

  13. Gates, F.I. and S. Linn (1977) J. Biol. Chem. 252: 1647–1653

    PubMed  CAS  Google Scholar 

  14. Warner, H.R. and J.E. Barnes (1966) Proc. Nat. Acad. Sci. USA 56: 1233–1240

    Article  PubMed  CAS  Google Scholar 

  15. Sadowski, P.D., H.R. Warner, K. Hercules, J.L. Munro, S. Mendelsohn, and J.S. Wiberg (1971) J. Biol. Chem. 246: 3431–3433

    PubMed  CAS  Google Scholar 

  16. Sadowski, P.D. and D. Vetter (1973) Virology 54: 544–546

    Article  PubMed  CAS  Google Scholar 

  17. Kutter, E., A. Beug, R. Sluss, L. Jensen and D. Bradley (1975) J. Mol. Biol. 99: 591–607

    Article  PubMed  CAS  Google Scholar 

  18. Snyder, L., L. Gold and E. Kutter (1976) Proc. Nat. Acad. Sci. USA 73: 3098–3102

    Article  PubMed  CAS  Google Scholar 

  19. Price, A.R. and H.R. Warner (1969) Virology, 37: 882–892

    Article  Google Scholar 

  20. Lindahl, T., S. Ljungquist, W. Siegert, B. Nyberg and B. Sperens (1977) J. Biol. Chem. (in press)

    Google Scholar 

  21. Dunham, L.F. and A.R. Price (1974) Biochemistry 13: 2667–2672

    Google Scholar 

  22. Ley, A.N., H.R. Warner and P. Kahn (1972) Can. J. Microbiol. 18: 375–384

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry College of Biological Sciences, University of Minnesota, St. Paul, Minnesota, 55108, USA

    Huber R. Warner & Bruce K. Duncan

Authors
  1. Huber R. Warner
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  2. Bruce K. Duncan
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Editor information

Editors and Affiliations

  1. Imperial Cancer Research Fund, London, England

    Ian Molineux

  2. University of Paris, Paris, France

    Masamichi Kohiyama

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© 1978 Plenum Press, New York

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Warner, H.R., Duncan, B.K. (1978). In Vivo Synthesis and Properties of Uracil-Containing DNA. In: Molineux, I., Kohiyama, M. (eds) DNA Synthesis. NATO Advanced Study Institutes Series, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0844-7_19

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  • DOI: https://doi.org/10.1007/978-1-4684-0844-7_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-0846-1

  • Online ISBN: 978-1-4684-0844-7

  • eBook Packages: Springer Book Archive

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