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Methods of Cell Separation pp 307–354Cite as

Partition of Cells in Two-Polymer Aqueous Phases: A Surface Affinity Method for Cell Separation

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Part of the book series: Biological Separations ((BIOSEP))

Abstract

This chapter describes a highly sensitive and versatile method for the separation and subfractionation of cell populations based on a variety of surface properties (e.g., surface charge, membrane lipid composition, presence of specific receptors). Furthermore, subtle changes in membrane surface properties can be traced that occur as a function of normalin vivo processes (i.e., differentiation, maturation, age) or in vitro treatments.

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Author information

Authors and Affiliations

  1. Laboratory of Chemical Biology, Veterans Administration Hospital, 90822, Long Beach, California, USA

    Harry Walter

  2. Department of Physiology, College of Medicine, University of California, 92717, Irvine, California, USA

    Harry Walter

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  1. Harry Walter
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Editor information

Editors and Affiliations

  1. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA

    Nicholas Catsimpoolas

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© 1977 Plenum Press, New York

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Walter, H. (1977). Partition of Cells in Two-Polymer Aqueous Phases: A Surface Affinity Method for Cell Separation. In: Catsimpoolas, N. (eds) Methods of Cell Separation. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0820-1_8

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  • DOI: https://doi.org/10.1007/978-1-4684-0820-1_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-0822-5

  • Online ISBN: 978-1-4684-0820-1

  • eBook Packages: Springer Book Archive

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