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Action of Ultrasound on Isolated Cells and Cell Cultures

  • C. R. Hill

Abstract

Understanding of the experimental evidence on the effects of ultrasound on living cells calls for an appreciation of the various biophysical mechanisms that may be involved. These include heat, cavitation in its various forms, microstreaming and other possible but unsubstantiated mechanisms. Biological endpoints that have been studied in relation to these mechanisms include cell disintegration, modification of proliferation patterns, genetic mutation, induction of chromosomal aberrations, modification of the cell membrane and of intracellular organelles and modification of cell function and radiosensitivity.

Tentative, concensus conclusions from published experimental data in this field are that: (1) mutation and chromosomal abnormalities have not been consistently demonstrated to result from ultrasonic exposure, (2) for producing cell death and a variety of reversible and irreversible non-genetic cellular modifications, cavitation can be a very effective mechanism, (3) other non-thermal mechanisms may operate but have not been consistently and repeatably demonstrated.

Human beings are more than cell cultures writ large and extrapolators operate in this territory at their own peril.

Keywords

Chromosomal Aberration Mitotic Index Isolate Cell Ultrasonic Irradiation Ultrasonic Field 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Webster, E.: Cavitation. Ultrasonics 1 (1963) 39.CrossRefGoogle Scholar
  2. 2.
    Clarke, P.R. and HILL, CR.: J.acoust.Soc.Amer. 47 (1970) 649.CrossRefGoogle Scholar
  3. 3.
    Hill, CR.: J.acoust.Soc.Amer. 52 (1972) 667.CrossRefGoogle Scholar
  4. 4.
    Hill, CR.: Detection of cavitation, in Interaction of Ultrasound and Biological Tissues p.199-200 DHEW Publication (FDA) 73-8008 (1973).Google Scholar
  5. 5.
    Clarke, P.R.: Studies of the biological effects of ultrasound and of synergism between ultrasound and x-rays. PhD Thesis, University of London, England (1969).Google Scholar
  6. 6.
    Coakley, W.T., Hampton, D. and Dunn, F.: J.acoust. Soc.Amer. 50 (1971) 1546.CrossRefGoogle Scholar
  7. 7.
    Clarke, P.R. and Hill, C.R. Expl.Cell Res. 58 (1969) 443.CrossRefGoogle Scholar
  8. 8.
    Thacker, J.: Curr.Topics in Radn. Res.Quarterly 8 (1973) 235.Google Scholar
  9. 9.
    Thacker, J.: Brit.J. Radiol. 47 (1974) 130.CrossRefGoogle Scholar
  10. 10.
    Brit. J. Radiol. 45 (five related papers) (1972) 319-342.Google Scholar
  11. 11.
    Macintosh, I.J.C.: (personal communication).Google Scholar
  12. 12.
    Taylor, K.J.W. and Newman, D.L.: Phys. Med. Biol. 17 (1972) 270.CrossRefGoogle Scholar
  13. 13.
    Joshi, G.P., Hill, C.R. and Forrester, J.A.: Ultrasound in Med. and Biol. 1, (1973) 45.CrossRefGoogle Scholar
  14. 14.
    Taylor, K.J.W. and Pond, J.: Primary sites of ultrasonic damage on cell systems. Interactions of Ultrasound and Biological Tissues, p.87-92, DHEW Publication (FDA) 73-8008 (1973).Google Scholar
  15. 15.
    Clarke, P.R., Hill, C.R. and Adams, K.: Brit. J. Radiol. 43 (1970) 97.CrossRefGoogle Scholar
  16. 16.
    Dunn, F. and Hawley, S.A. Ultrahigh frequency acoustic waves in liquids. Ultrasonic Energy (Kelly, E. Ed.) p.66-76. (Univ. of Illinois Press) (1965).Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • C. R. Hill
    • 1
  1. 1.Physics Division, Institute of Cancer ResearchRoyal Marsden HospitalSutton, SurreyUK

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