Computer-Assisted Imaging

Abstract

Most biologists interested in morphometric analysis purchase programs to do the work, and a specific knowledge of all the algorithms and statistical calculations behind their operation is not critical. The programs are booted up, the menus are consulted to see what operations are possible, and the operator instructs the computer to perform certain operations from the menu. At the same time, if the operator does not possess knowledge concerning the types of questions that may be asked, the limitations inherent in the sampling techniques being used, and the general underlying assumptions necessary to computer-assisted image analysis, the equipment may not be used to its ultimate capacity. The purpose of this chapter is to give an overview of the capabilities and a few of the shortcomings of computer-assisted image analysis procedures used today. Three texts (Russ, 1986, 1990; Hader, 1991) cover most aspects of the mechanics behind image analysis and address specific applications in great detail. They are recommended reading prior to embarking on a project involving computer-assisted image analysis. The text by Misell (1978) was written prior to most of the computerassisted analysis equipment currently available but deals extensively with principles and methods for image enhancement and analysis not confined to computers.