Abstract
Of the [3H]choline newly taken up into small slices of frontal cortex, a larger fraction is acetylated in samples from adult rats than in samples from 7–14 day olds (Atterwill & Prince, 1978). This developmental change is apparent whether acetylation is expressed as a fraction of total uptake, or of uptake corrected for that in sodium-free medium (Kalaria et al., 1980). The increasing acetylation with age may reflect increasing concentrations of choline acetyltransferase (ChAT, EC 2.3.1.6) in the cortex during development. More specifically, it may reflect increasing concentrations of the basic molecular form of rat brain ChAT (Atterwill & Prince, 1978), which has appreciable affinity for synaptosomal membrane fragments (Fonnum & Malthe-Sørenssen, 1973).
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References
Ansell, G.B. & Spanner, S. (1980) The activity of glycerophosphocholine phosphodiesterase in brain tissue, In: Int. Symposium on Cholinergic Mechanism (Pepeu, G. & Ladinsky, H., eds). Florence, 1980 (in press), Plenum Press, New York.
Atterwill, C.K. & Prince, A.K. (1977) Choline uptake, choline acetyltransferase and the acetylation of choline in rat brain Br. J. Pharmacol. 62, 398P.
Atterwill, C.K. & Prince, A.K. (1978) Multiple forms of choline acetyltransferase and the high affinity uptake of choline in brain of developing and adult rats. J. Neurochem. 31, 719–725.
Burgess, E.J., Atterwill, C.K. & Prince, A.K. (1978) Choline acetyltransferase and the high affinity uptake of choline in corpus striatum of reserpinised rats. J. Neurochem, 31, 1027 -1033.
Diamond, I. & Fishman, R.W. (1973) Development of Na+ -stimulated glucose oxidation in synaptosomes. J. Neurochem. 21, 1043–1050.
Fonnum, F., (1975) A rapid radiochemical method for the determination of choline acetyltransferase. J. Neurochem. 24, 407–409.
Fonnum, F. & Malthe-Sørenssen, D. (1973) Membrane affinities and subcellular distribution of the different molecular forms of choline acetyl-transferase from rat. J. Neurochem. 20, 1351–1359.
Herberg, R.J. (1960) Determination of carbon-14 and tritium in blood and other whole tissues. Liquid scintillation counting of tissues. Analyt. Chem. 32, 42–46.
Jope, R.S. (1979) High affinity choline transport and acetyl-CoA production in brain and their roles in the regulation of acetylcholine synthesis. Brain Research Rev. 1. 313–344.
Kalaria, R.N., Kotas, A.M., Prince, A.K., Reynolds, R. & Wong, P.T-H. (1980a) Choline uptake and choline acetyltransferase in brain of developing rats made hypothyroid with propylthiouracil, In: Int. Symposium on Cholinergic Mechanisms, (Pepeu, G. & Ladinsky, H., eds). Florence, 1980, (in press) Plenum Press, New York.
Kalaria, R.N., Kotas, A.M., Prince, A.K., Reynolds, R & Wong, P.T-H.(1980b) Choline uptake, choline acetyltransferase and the synthesis of ACh in brain of normally developing and hypothyroid rats. In: Proceedings of the 1st meeting of the International Society for Developmental Neuroscience, (Gombos G. & Vincendon, G., eds) Strasbourg, 1980, (in press).
Kessler, P.D. & Marchbanks, R.M. (1979) Choline transport is not coupled to acetylcholine synthesis. Nature (Lond.) 279, 542–544.
Malthe-Sørenssen, D. (1976) Molecular properties of choline acetyltransferase from different species investigated by isoelectric focusing and ion exchange adsorption. J. Neurochem, 26, 861–865.
Malthe-Sørenssen, D. & Fonnum, F. (1972) Multiple forms of choline acetyltransferase in several species demonstrated by isoelectric focusing. Biochem. J. 127, 229–236.
Marchbanks, R.M. & Wonnacott, S. (1979) Relation of choline uptake to acetylcholine synthesis and release. In: The Cholinergic Synapse. Progress in Brain Research, 49, (Tuček, S., ed). pp 77–88, Elsevier, Amsterdam.
Marchbanks, R.M. (1980) Interaction of choline transport with acetylcholine release and synthesis, In: Int. Symposium on Cholinergic Mechanisms (Pepeu, G. , Ladinsky, H., eds) Plenum, Press, New York.
Massarelli, R., Wong, T.Y., Froissart, C. & Robert, J. (1979) Choline uptake and metabolism by nerve cell cultures, In: The Cholinergic Synapse.Progress in Brain Research, 49, (Tuček, S., ed), pp 89–96, Elsevier, Amsterdam.
Massarelli, R., & Wong, T.Y. (1980) Choline uptake in nerve cultures and in synaptosomal preparations is regulated by the endogenous pool of choline, In: Int. Symposium on Cholinergic Mechanisms (Pepeu, G. & Ladinsky, H., eds). Florence, 1980 (in press) Plenum Press, New York.
Reynolds, R. & Prince, A.K. (1980) The uptake and acetylation of 3H-choline in brain of inbred strains of mice, In: Int. Symposium on Cholinergic Mechanisms (Pepeu, G. & Ladinsky,H., eds) Florence, 1980, (in press) Plenum Press, New York.
Shaikh, N.A. & Palmer, F.B.St.C. (1976) Deposition of lipids in the developing central and peripheral nervous systems of the chicken. J. Neurochem. 26, 597–603.
Wong, P.T-H. & Prince, A.K (1979) The membrane binding of choline acetyltransferase: uptake and acetylation of [3h] choline in rabbit cortical slices. Neuropharmacol. 18, 511–513.
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© 1981 C.J. Pycock and P.V. Taberner
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Atterwill, C.K., Prince, A.K., Reynolds, R., Wong, P.TH. (1981). Species Differences in the Acetylation of [3H] Choline in Cortical Slices. In: Pycock, C.J., Taberner, P.V. (eds) Central Neurotransmitter Turnover. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9778-0_11
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