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In Situ Hybridization for Detection of Viral Nucleic Acid in Cell Cultures and Tissues

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In Situ Hybridization in Brain

Abstract

In situ hybridization to isolated cells and tissue sections is an important technique for the localization of mammalian and viral mRNA and DNA. This report describes advances in tissue fixation allowing superior preservation of specimen morphology, concurrent immunohistochemistry and in situ hybridization, and enhanced sensitivity of mRNA detection. In addition, strategies for isotopic and non-isotopic DNA and RNA labeling and assays for mRNA quantitation are described in measles, visna, AIDS and papovavirus infections.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Microbiology and Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases and Infectious Diseases Branch, USA

    Howard E. Gendelman, Scott Koenig, Allen Aksamit & Sundarajan Venkatesan

  2. National Institute of Neurological and Communicative Diseases and Stroke, USA

    Howard E. Gendelman, Scott Koenig, Allen Aksamit & Sundarajan Venkatesan

  3. Neurology Service, VAMC, West Los Angeles, USA

    Wallace W. Tourtellotte, Peter Schmid & Paul Shapshak

  4. Wadsworth Division Neurology Department and Pediatrics Department, UCLA Schoool of Medicine, Los Angeles, CA, 90073, USA

    Wallace W. Tourtellotte, Peter Schmid & Paul Shapshak

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  1. Howard E. Gendelman
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  2. Scott Koenig
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  3. Allen Aksamit
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  5. Wallace W. Tourtellotte
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  7. Paul Shapshak
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Editor information

Editors and Affiliations

  1. Department of Neurology and Howard Hughes Medical Institute, USA

    George R. Uhl M.D., Ph.D.

  2. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA

    George R. Uhl M.D., Ph.D.

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© 1986 Plenum Press, New York

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Gendelman, H.E. et al. (1986). In Situ Hybridization for Detection of Viral Nucleic Acid in Cell Cultures and Tissues. In: Uhl, G.R. (eds) In Situ Hybridization in Brain. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9486-4_13

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