Expression of Beta 2 Microglobulin on the Surface of Mononuclear Cells in Patients with Acquired Immune Deficiency Syndrome (AIDS) and Aids-Related Complex (ARC)
Beta 2 microglobulin (B2-m) is a low molecular weight protein of 11,800 daltons and contains 100 amino acids. It was originally isolated from human urine and has been found in all body fluids (1–3). B2-m shows a homology with CH3 domain of immunoglobulin molecules and is associated with class I major histocompatibility complex molecules. B2-m is present on the surface of and synthesized by virtually all somatic cells. In vitro, both normal and malignant human cell lines and activated normal lymphocytes synthesize B2-m (4, 5). B2-m has been shown to play a role in T cell response to mitogens, soluble antigens and alloantigens (6–12). Acquired immune deficiency syndrome (AIDS) is characterized by several defects of T cell functions and clinically manifested by opportunistic infections and/or Kaposi’s sarcoma (KS) (13–16). Recently, increased plasma levels of B2-m in patients with AIDS and hemophilia have been described (17–19). In this study we have examined the expression of surface B2-m on the mononuclear cells (MNC) from patients with AIDS and AIDS-related complex (ARC), using a monoclonal antibody and fluorescence- activated cell sorter analyzer (20).
KeywordsAcquire Immune Deficiency Syndrome Acquire Immunodeficiency Syndrome Major Histocompatibility Complex Molecule Autologous Mixed Lymphocyte Reaction Human Monocyte Cell Line
Unable to display preview. Download preview PDF.
- 1.B. Berggard, L. Bjorck, R. Gigen, and L. Logdberg, Microglobulin, Scand J Clin Lab Invest 40 (Supp. 154): 13 (1980).Google Scholar
- 2.F.A. Karlsson, L. Wibell, and P.E. Erin, 32 microglobulin in clinical medicine, Scand J Clin Lab Invest 40 (Supp. 140): 27 (1980).Google Scholar
- 3.M.D. Paulik and R.A. Reisfeld, $2-micr0gl0bulin, Contemp Topics Mol Immunol 4; 157 (1975).Google Scholar
- 4.K. Nilsson, P.E. Evrin, I. Berggard, and J. Ponten, Involvement of lymphoid and non-lymphoid cells in the production of 32 microglobulin—a homologue of the constant domain of IgG, Nature (New Biol) 244: 44 (1973).Google Scholar
- 6.M.L. Bach, S.W. Huang, R. Hong, and M.D. Paulik, ß2~niicroglobulin association with lymphocyte receptors, Science 182: 1350 (1973).Google Scholar
- 9.B.G. Solheim, Association between 32Tn_HLA molecule and membrane structures responsible for lymphocyte activation, Transplant Rev 21: 35 (1974).Google Scholar
- 11.R.T. McCalmon, R.T. Kubo, and H.M. Grey, Effect of anti-ß2microglobulin on antigen and allogeneic lymphocyte-induced proliferation of human lymphocytes. J Immunol 114: 1766 (1975).Google Scholar
- 16.S. Gupta, S. Gillis, M. Thornton, and M. Goldberg, Autologous mixed lymphocyte reaction in man. XIV. Deficiency of the autologous mixed lymphocyte reaction in acquired immune deficiency syndrome (AIDS) and AIDS-related complex (ARC): In vitro effect of purified interleukin-1 and interleukin-2, Clin Exp Immunol 58: 395 (1984).PubMedGoogle Scholar
- 18.J.K. Kreiss, D.N. Lawrence, C.K. Kasper, A.L. Goldstein, P.H. Naylor, M.F. McLane, T-H. Lee, and M. Essex, Antibody to human T-cell leukemia virus membrane antigens, beta2-microglobulin levels, and thymosin alpha 1 levels in hemophiliacs and their spouses, Ann Int Med 100: 178 (1984).PubMedGoogle Scholar
- 20.S. Gupta, Monoclonal antibody-defined (-microglobulin positive mononuclear cells in AIDS, Scand J Immunol, in press.Google Scholar