Abstract
3-Hydroxy-3-methylglutaryl CoA reductase (HMG-CoA R), the microsomal enzyme catalyzing the synthesis of mevalonate from 3-hydroxy-3-methylglutaryl CoA, is known as one of 1the key enzymes in the regulation of cholesterol biosynthesis. Many authors have demonstrated that the enzyme activity is modulated by a phosphorylation-dephosphorylation reaction.1 The equilibrium between the two forms of the enzyme has been proposed as a rapid mechanism for the regulation of cholesterol synthesis. Studies in vitro for testing the inhibitory effects of oxygenated sterols and drugs have been mainly carried out using hepatocyte cultures2,3 However in these cell cultures, the HMG-CoA R was reported to be only in its dephosphorylated active form, whereas in the 4rat liver, the enzyme is present mainly as the inactive form (80%)4.
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© 1985 Plenum Press, New York
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Cighetti, G., Galli, G., Paroni, R., Kienle, M.G. (1985). Effects of Inhibitors of Cholesterol Biosynthesis in Isolated Rat Hepatocytes. In: Galli, G., Bosisio, E. (eds) Liver, Nutrition, and Bile Acids. NATO ASI Series, vol 90. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9427-7_17
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DOI: https://doi.org/10.1007/978-1-4615-9427-7_17
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