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Ionic Currents Activated by Voltage and by Intracellular Calcium: A Voltage Clamp Study in Solitary Vertebrate Photoreceptors

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Photoreceptors

Part of the book series: NATO Asi Series ((NSSA,volume 75))

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Abstract

Solitary rod inner segments were obtained by enzymatic dissociation of the tiger salamander retina. Individual membrane currents were studied with the single-electrode voltage-clamp technique and with pharmacological agents. Extracellular TEA blocked an outward current which was activated, by depolarization and carried predominantly by potassium ions. Extracellular caesium blocked an inward current which was activated by hyperpolarization and carried by sodium and potassium ions. An inward calcium current was activated by depolarization and could be blocked by extracellular cobalt. Intracellular accumulation of calcium activates two other currents: one is blocked by intracellular caesium and is most likely a calcium- activated potassium current; the current remaining in the presence of intracellular caesium is carried, in part, by chloride. The five currents described can all be activated in the range of voltages in which rod photoreceptors normally operate.

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References

  • Attwell, D., & Wilson, M. (1980). Behavior of the rod network in the tiger salamander retina mediated by membrane properties of individual rod, J. Physiol., 309, 287–315.

    PubMed  CAS  Google Scholar 

  • Bader, C.R., Maeish, P.R. & Schwartz, E.A. (1978). Responses to light of solitary rod photoreceptors isolated from the tiger salamander retina, Proc. natn. Acad. Sci. U.S.A., 75, 3507–3511.

    Article  CAS  Google Scholar 

  • Bader, C.R., MacLeish, P.R. & Schwartz, E.A. (1979). A voltage- clamp study of the light response in solitary rods of the tiger salamander, J. Physiol., 296, 1–26.

    PubMed  CAS  Google Scholar 

  • Baylor, D.A. & Fuortes, M.G.F. (1970). Electrical responses of single cones in the retina of the turtle, J. Physiol., 207, 77–92.

    PubMed  CAS  Google Scholar 

  • Baylor, D.A., Fuortes, M.G.F. & O’Bryan, P.M. (1971). Receptive fields of cones in the retina of the turtle, J. Physiol., 214, 265–294.

    PubMed  CAS  Google Scholar 

  • Baylor, D.A. & Hodgkin, A.L. (1973). Detection and resolution of visual stimuli by turtle photoreceptors, J. Physiol., 234, 163–198.

    PubMed  CAS  Google Scholar 

  • Baylor, D.A., Lamb, T.D. & Yau, K.-W. (1979). The membrane current of single rod outer segments, J. Physiol., 288, 589–611.

    PubMed  CAS  Google Scholar 

  • Brehm, P., Eckert, R. & Tillotson, D. (1980). Calcium-mediated inactivation of calcium current in Paramecium, J. Physiol., 306, 193–203.

    PubMed  CAS  Google Scholar 

  • Brown, J.E. & Pinto, L.H. (1974). Ionic mechanism for the photoreceptor potential of the retina of Bufo marinus, J. Physiol., 236, 575–591.

    PubMed  CAS  Google Scholar 

  • Capovilla, M., Cervetto, L. & Torre, V. (1980). Effects of changing external potassium and chloride concentrations on the photoresponses of Bufo bufo rods, J. Physiol., 307, 529–551.

    PubMed  CAS  Google Scholar 

  • Cervetto, L. & Piccolino, M. (1974). Synaptic transmission between photoreceptors and horizontal cells in the turtle retina. Science, 183, 417–418.

    Article  PubMed  CAS  Google Scholar 

  • Copenhagen, D.R. & Owen, W.G. (1976). Functional characteristics of lateral interactions between rods in the retina of the snapping turtle, J. Physiol., 259, 251–282.

    PubMed  CAS  Google Scholar 

  • Detwiler, P.B., Hodgkin, A.L. & Maaughton, P.A. (1978). A surprising property of the electrical spread in the network of rods in the turtle retina. Nature, (Lond.), 274, 562–565.

    Article  CAS  Google Scholar 

  • Dunlap, K. & Fischbach, G.D. (1978). Neurotransmitters decrease the calcium component of sensory neurone action potentials, Nature (Lond.), 276, 837–839.

    Article  CAS  Google Scholar 

  • Fain, G.L., Gold, G.H. & Dowling, J.E. (1975). Receptor coupling in the toad retina. Cold Spring Harb. Symp. quant. Biol., 40, 547–561.

    Article  Google Scholar 

  • Fain, G.L., Quandt, F.N. & Gershenfeld, H.M. (1977). Calcium-dependent regenerative responses in rods. Nature, Lond., 269, 707–710.

    Article  CAS  Google Scholar 

  • Fain, G.L., Quandt, F.N., Bastian, B.L. & Gerschenfeld, H.M. (1978). Contribution of a caesium-sensitive conductance increase to rod response. Nature, Lond., 272, 467–469.

    Article  CAS  Google Scholar 

  • Fain, G.L., Gerschenfeld, H.M. & Quandt, F.N. (1980). Calcium spikes in rods, J. Physiol., 303, 495–513.

    PubMed  CAS  Google Scholar 

  • Fain, G.L. & Quandt, F.N. (1980). The effects of tetraethylammonium and cobalt ions on responses to extrinsic current in toad rods, J. Physiol., 303, 515–533.

    PubMed  CAS  Google Scholar 

  • Fuortes, M.G.F., Schwartz, E.A. & Simon, E.J. (1973). Colour-dependence of cone responses in the turtle retina, J. Physiol., 234, 199–216.

    PubMed  CAS  Google Scholar 

  • Hagins, W.A., Penn, R.D. & Yoshikami, S. (1970). Dark current and photocurrent in rat retinal rods, Biophys. J., 10, 380–412.

    Article  PubMed  CAS  Google Scholar 

  • Hagiwara, S., Miyazaki, S. & Rosenthal, N.P. (1976). Potassium current and the effect of caesiimi on this current during anomalous rectification of the egg cell membrane of a starfish, J. Gen. Physiol., 67, 621–638.

    Article  PubMed  CAS  Google Scholar 

  • Hagiwara, S. & Nakajima, S. (1966). Effects of the intracellular Ca ion concentration upon the excitability of the muscle fiber membrane of a barnacle, J. Gen. Physiol., 49, 807–818.

    Article  PubMed  CAS  Google Scholar 

  • Horowicz, P., Gage, P.W. & Eisenberg, R.S. (1968). The role of the electrochemical gradient in determining potassium fluxes in frog striated muscle, J. Gen. Physiol., 51, 193S-203S.

    PubMed  CAS  Google Scholar 

  • Kaneko, A. & Shimazaki, H. (1976). Synaptic transmission from photoreceptors to bipolar and horizontal cells in the carp retina. Cold Spring Harb. Quant. Biol., 40, 537–546.

    Article  CAS  Google Scholar 

  • Kostyuk, P.G. & Krishtal, O.A. (1977). Effects of calcium and calcium-chelating agents on the inward and outward current in the membrane of molluscan neurones, J. Physiol., 270, 569–580.

    PubMed  CAS  Google Scholar 

  • Lasansky, A. (1981). Synaptic action mediatiiag cone responses to annular illumination in the retina of the larval tiger salamander, J. Physiol., 310, 205–214.

    PubMed  CAS  Google Scholar 

  • Meech, R.W. (1978). Calcium-dependent potassium activation in nervous tissues, Ann. Rev. Biophys. Bioeng., 7, 1–18.

    Article  CAS  Google Scholar 

  • Miyazaki, S., Takahashi, K., Tsuda, K. & Yoshii, M. (1974). Analysis of non-linearity observed in the current-voltage relation of the tunicate embryo, J. Physiol., 238, 55–77.

    PubMed  CAS  Google Scholar 

  • O’Bryan, P.M. (1973). Properties of the depolarizing synaptic potential evoked by peripheral illumination in cones of the turtle retina, J. Physiol., 235, 207–223.

    PubMed  Google Scholar 

  • Pellmar, T.C. (1981). Transmitter control of voltage-dependent currents. Life Sci., 28, 2199–2205.

    Article  CAS  Google Scholar 

  • Piccolino, M. & Gerschenfeld, H.M. (1977). Lateral interactions in the outer plexiform layer of turtle retinas after atropine block of horizontal cells. Nature, Lond., 268, 259–261.

    Article  CAS  Google Scholar 

  • Reuter, H. & Scholz, H. (1977 a). A study of the ion selectivity and the kinetic properties of the calcium-dependent slow inward current in mammalian cardiac muscle, J. Physiol., 262, 17–47.

    Google Scholar 

  • Reuter, H. & Scholz, H. (1977 b). The regulation of the calcium conductance of cardiac muscle by adrenaline, J. Physiol., 264, 49–62.

    PubMed  CAS  Google Scholar 

  • Schwartz, E.A. (1976). Electrical properties of the rod syncytium in the retina of the turtle, J. Physiol., 257, 379–406.

    PubMed  CAS  Google Scholar 

  • Sillman, A.J., Ito, H. & Tomita, T. (1969). Studies on the mass receptor potential of the isolated frog retina. II. On the basis of the ionic mechanism. Vision Res., 9, 1443–1451.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, R.C. (1972). Electrogenic sodium pump in nerve and muscle cells, Physiol. Rev., 52, 563–594.

    PubMed  CAS  Google Scholar 

  • Tillotson, D. & Horn, R. (1978). Inactivation without facilitation of calcium conductance in caesium-loaded neurones of Aplysia, Nature, Lond., 273, 312–314.

    Article  CAS  Google Scholar 

  • Tillotson, D. (1979). Inactivation of Ca conductance dependent on entry of Ca ions in moluscan neurones, Proc. natn. Acad. Sci. U.S.A., 76, 1497–1500.

    Article  CAS  Google Scholar 

  • Tomita, T. (1965). Electrophysiological study of the mechanisms subserving color coding in the fish retina. Cold Spring Harb. Symp. quant. Biol., 30, 559–566;

    Article  PubMed  CAS  Google Scholar 

  • Trifonov, Y.A. (1968). Study of synaptic transmission between photoreceptor and horizonatal cell by electrical stimulation of the retina, Biofizica, 13, 809–817.

    CAS  Google Scholar 

  • Werblin, F.S. (1979). Time- and voltage-dependent ionic components of the rod response, J. Physiol., 294, 613–626.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Départment de Physiologie, Centre Médical Universitaire, 21 rue Lombard, 1211, Géneve 4, Switzerland

    C. R. Bader & D. Bertrand

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  1. C. R. Bader
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  2. D. Bertrand
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Editors and Affiliations

  1. University of Genova, Genova, Italy

    A. Borsellino

  2. Institute of Neurophysiology of the CNR, Pisa, Italy

    L. Cervetto

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© 1984 Plenum Press, New York

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Bader, C.R., Bertrand, D. (1984). Ionic Currents Activated by Voltage and by Intracellular Calcium: A Voltage Clamp Study in Solitary Vertebrate Photoreceptors. In: Borsellino, A., Cervetto, L. (eds) Photoreceptors. NATO Asi Series, vol 75. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9382-9_12

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  • DOI: https://doi.org/10.1007/978-1-4615-9382-9_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9384-3

  • Online ISBN: 978-1-4615-9382-9

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