Abstract
PHA-stimulated human peripheral lymphocytes were used as a model system for assessing the in vitro effects of calcium cyclamate. Techniques of autoradiography, cytological staining, cell counting, liquid scintillation and karyotyping were used to study the cytogenetic damage and biochemical effects of calcium cyclamate when assayed in 24 hour intervals for 96 hours. The cells were exposed to 10−2 and 10−3 molar concentrations of calcium cyclamate in TC 199 medium with fetal calf serum and antibiotics. These studies were carried out in three (3) phases. Phase I was primarily orientation studies of the effects of cyclamates and included running preliminary test checks, the establishment of parameters of dosage, assessing growth patterns and selecting key chromosomal aberrations. Sixty four (64) of the metaphase spreads showed morphologically detectable changes and aberrations. It was also noted that the addition of cyclamate increased mitotic rate of lymphocyte cells in cultures.
A portion of this paper was presented before the Second Inter national Cell Culture Congress — In Support of BioScience — University of Alabama in Birmingham, September, 1981. Supported by grants from NIH/MBS Program #2-S06-RR-08090.
Professor at Virginia State University to whom requests for reprints should be sent.
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© 1984 Plenum Press, New York
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Jemison, E.W., Brown, K., Rivers, B., Knight, R. (1984). Cytogenetic Effects of Cyclamates. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_6
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