Skip to main content
SpringerLink
Log in

Angiotensin Receptors and the Control of Na+ and K+ Transport in Cultured Aortic Smooth Muscle and Brain Microvessel Cells

  • Chapter
  • First Online:
Eukaryotic Cell Cultures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

Abstract

We examined the effect of angiotensin on Na+ and K+ transport by cultures of aortic smooth muscle and brain microvessel cells. Angiotensin II (AII) and angiotensin III (AIII) stimulated net Na+ uptake, which was assayed in the presence of ouabain to block Na+ efflux via the Na+ -K+ pump. The combination of saturating concentrations of AII and AIII produced no greater stimulation of net Na+ uptake than either angiotensin by itself. AII also stimulated ouabain-sensitive 86Rb+ uptake by cultured aortic smooth muscle and brain microvessel cells. AII was not as effective as monensin, a Na+ ionophore, in stimulating the Na+ -K+ pump. In the presence of monensin, AII had no effect on ouabain-sensitive 86Rb+ uptake. These data are consistent with previous observations suggesting that AII stimulates the Na+ -K+ pump by supplying it with more of its rate-limiting substrate, Na+.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Filo, R.S., Bohr, D.F., and Ruegg, J.C. 1974. Glycerinated skeletal and smooth muscle: calcium and magnesium dependence. Science 147:1581–1583.

    Article  Google Scholar 

  2. Ruegg, J.G. 1971. Smooth muscle tone. Physiol. Rev. 51:201–248.

    Article  Google Scholar 

  3. Johansson B. 1978. Processes inved in vascular smooth muscle contraction and relaxation. Circ. Res. 43I14–I20

    Google Scholar 

  4. Devynck, M.A. and Meyer, P. 1976. Angiotensin receptors in vascular tissue. Am. J. Med. 61:758–767.

    Article  Google Scholar 

  5. Schultz, G.A., Galardy, R.W., and Jamieson, J.D. 1981. Biological activity of an angiotensin II-ferritin conjugate on rabbit aortic smooth muscle. Biochem. 20:3412–3418.

    Article  Google Scholar 

  6. Glossman, H., Baukal, A.J., and Catt, K.J. 1974. Properties of angiotensin II receptors in the bovine and rat adrenal cortex. J. Biol. Chem. 249:825–834.

    Google Scholar 

  7. Sraer, J.D., Sraer, J., Ardaillous, R., and Minoune, O. 1974. Evidence for renal glomerular receptors for angiotensin II. Kidney Int. 6:241–246.

    Article  Google Scholar 

  8. Bennett, J.P., Jr. and Snyder, S.H. 1976. Angiotensin II binding to mammalian brain membranes. J. Biol. Chem. 251:7423–7430.

    Google Scholar 

  9. Gunther, S., Gimbrone, M.A., Jr. and Alexander, R.W. 1980. Identification and characterization of the high affinity vascular angiotensin II receptor in rat mesenteric artery. Circ. Res. 47:278–286.

    Article  Google Scholar 

  10. Van Breeman, C., Aaronson, P. and Loutzenhiser, R. 1979. Sodium-calcium interaction in mammalian smooth muscle. Pharmacol. Rev. 30:167–208.

    Google Scholar 

  11. Blaustein, M.P. 1977. Sodium ions, calcium ions, blood pressure regulation, and hypertension: a reassessment and a hypothesis. Am. J. Physiol. 232:C165–C173.

    Article  Google Scholar 

  12. Friedman, S.M. and Allardyce, D.B. 1962. Sodium and tension in an artery segment. Circ. Res. 11:84–89.

    Google Scholar 

  13. Guignard, J.P., and Friedman, S.M. 1971. Vascular ionic effects of angiotensin II in the rat. Proc. Soc. Exp. Biol. Med. 137:157–160.

    Article  Google Scholar 

  14. Turker, R.K., Page, I.H., and Khairallah, P.A. 1967. Angiotensin alteration of sodium fluxes in smooth muscle. Arch. Int. Pharmacodyn. Ther. 165:394–404.

    Google Scholar 

  15. Brock, T.A., Lewis, L.J., and Smith, J.B. 1982. Angiotensin increases Na+ entry and Na+-K+ pump activity in cultures of smooth muscle from rat aorta. Proc. Natl. Acad. Sci. U.S.A. 79:1438–1442.

    Article  Google Scholar 

  16. Brendel, K., Meezan, E. and Carlson, E.C. 1974. Isolated brain microvessels: a purified, metabolically active preparation from bovine cerebral cortex. Science 185:953–955.

    Article  Google Scholar 

  17. DeBault, L.E., Kahn, L.E., Frommes, S.P. and Cancilla, P.A. 1979. Cerebral microvessels and derived cells in tissue culture: isolation and preliminary characterization. In Vitro 15:473–487.

    Article  Google Scholar 

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  19. Thomas, R.C. 1972. Electrogenic sodium pump in nerve and muscle cells. Physiol. Rev. 52:563–594.

    Article  Google Scholar 

  20. Smith, J.B. and E. Rozengurt. 1978. Serum stimulates the Na+-K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc. Natl. Acad. Sci. U.S.A. 75:5560–5564.

    Article  Google Scholar 

  21. Anderson, D.K. 1976. Cell potential and the sodium-potassium pump in vascular smooth muscle. Fed. Proc. 35:1293–1297.

    Google Scholar 

  22. Fleming, W.W. 1980. The electrogenic Na+, K+-pump in smooth muscle: physiological and pharmacological significance. Ann. Rev. Pharmacol. Toxicol. 20:129.

    Article  Google Scholar 

  23. Lin, S.Y., and Goodfriend, T.S. 1970. Angiotensin receptors. Am. J. Physiol. 218:1319–1328.

    Article  Google Scholar 

  24. LeMorvan, P., and Palaic, D. 1975. Characterization of the angiotensin receptor in guinea pig aorta. J. Pharmacol. Exp. Ther. 195:167–175.

    Google Scholar 

  25. Kaplan, J. 1981. Polypeptide-binding membrane receptors: analysis and classification. Science 212:14–20.

    Article  Google Scholar 

  26. Pastan, I.H. and Willingham, M.C. 1981. Receptor mediated endocytosis of hormones in cultured cells. Ann. Rev. Physiol. 43:239–250.

    Article  Google Scholar 

  27. Alexander, R.W., Hyman, S., Atkinson, W. and Gimbrone, M.A., Jr. 1980. Regulation of angiotensin II receptors in cultured vascular smooth muscle cells. Circulation 62: (Suppl. III):90 (abs.).

    Google Scholar 

  28. Maxfield, F.R., Willingham, M.C., Davies, P.J.A. and Pastan, I. 1979. Amines inhibit the clustering of α2-macroglobulin on the fibroblast cell surface. Nature (London) 277:661–663.

    Article  Google Scholar 

  29. Bolton, T.B. 1979. Mechanisms of action of transmitters and other substances in smooth muscle. Physiol. Rev. 59:606–718.

    Article  Google Scholar 

  30. Ohasi, H., Nonomura, Y. and Ohaga, A. 1976. Effects of angiotensin, bradykinin, and ocytocin on electrical and mechanical activities in the Taenia coli of the guinea pig. Jap. J. Pharmacol. 17:247–257.

    Article  Google Scholar 

  31. Somlyo, A.P., and Somlyo, A.V. 1971. Electrophysiological correlates of the inequality of maximal vascular smooth muscle contractions elicited by drugs. In: Proc. Symp. Physiol. Pharmacol. Vascular Neuroeffector systems. eds. Bevan, J.A., Furchgott, R.F., Maxwell, R.A., and Somlyo, A.P. Karger, Basel, pp. 216–228.

    Chapter  Google Scholar 

  32. Hamon, G. and Worcel, M. 1979. Electrophysiological study of the action of angiotensin II on the rat myometrium. Circ. Res. 45:234–243.

    Article  Google Scholar 

  33. Zelcer, E. and Sperelakis, N. 1981. Angiotensin induction of active responses in cultured reaggregates of rat aortic smooth muscle cells. Blood Vessels, In press.

    Google Scholar 

  34. Baudoin, M., Meyer, P., Fesmandijian, S. and Morgat, J.L. 1972. Calcium release induced by interaction of angiotensin with its receptors in smooth muscle cell microsomes. Nature 235:336–338.

    Article  Google Scholar 

  35. Elliot, M.E., and T.L. Goodfriend. 1981. Angiotensin alters 45Ca2+ fluxes in bovine adrenal glomerulosa cells. Proc. Natl. Acad. Sci. U.S.A. 78:3044–3048.

    Article  Google Scholar 

  36. Peach, M.J. 1977. Renin-angiotensin system: biochemistry and mechanisms of action. Physiol. Rev. 57:313–370.

    Article  Google Scholar 

  37. Schelling, P., Ganten, D., Speck, G. and Fisher, H. 1979. Effects of angiotensin II and angiotensin II antagonist saralasin on cell growth and renin in 3T3 and SV3T3 cells. J. Gen. Physiol. 98:503–514.

    Google Scholar 

  38. Gill, G.N., III Simonian, M.H. 1977. Angiotensin stimulation of bovine adrenocortical cell growth. Proc. Natl. Acad. Sci. U.S.A. 74:5569–5573.

    Article  Google Scholar 

  39. Schiffrin, E.L., Lis, M., Gutkowska, J. and Genest, J. 1981. Role of Ca2+ in response of adrenal glomerulosa cells to angiotensin II, ACTH, K+, and ouabain. Am. J. Physiol. 241:E42–E46.

    Article  Google Scholar 

  40. Leffert, L.H., Editor. 1980. Growth regulation by ion fluxes. Ann. N.Y. Aca. Sci. 339:1–335.

    Google Scholar 

  41. Smith, J.B. and Rozengurt, E. 1978: Lithium transport by fibroblastic mouse cells: characterization and stimulation by serum and purified growth factors in quiescent cultures. J. Cell. Physiol. 97:441–449.

    Article  Google Scholar 

  42. Smith, J.B. 1980. The Na+ transporter in normal and transformed 3T3 cells: Kinetics and modulation by Ca ions. Dev. Biochem. 14:101–102.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cardiovascular Research and Training Center and Department of Pharmacology, Univerisity of Alabama in Birmingham, Birmingham, Alabama, 35294, USA

    Tommy A. Brock & Jeffrey B. Smith

Authors
  1. Tommy A. Brock
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey B. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    Ronald T. Acton  & J. Daniel Lynn  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Plenum Press, New York

About this chapter

Cite this chapter

Brock, T.A., Smith, J.B. (1984). Angiotensin Receptors and the Control of Na+ and K+ Transport in Cultured Aortic Smooth Muscle and Brain Microvessel Cells. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_25

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_25

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-9378-2

  • Online ISBN: 978-1-4615-9376-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation